Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NHM04_RS12395 Genome accession   NZ_CP100350
Coordinates   2822135..2823280 (+) Length   381 a.a.
NCBI ID   WP_254264101.1    Uniprot ID   A0A9J7A470
Organism   Gilvimarinus sp. DA14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2817135..2828280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHM04_RS12375 (NHM04_12375) - 2818774..2819364 (-) 591 WP_254264099.1 YggT family protein -
  NHM04_RS12380 (NHM04_12380) proC 2819385..2820203 (-) 819 WP_256526535.1 pyrroline-5-carboxylate reductase -
  NHM04_RS12385 (NHM04_12385) - 2820200..2820922 (-) 723 WP_254264100.1 YggS family pyridoxal phosphate-dependent enzyme -
  NHM04_RS12390 (NHM04_12390) pilT 2821012..2822046 (+) 1035 WP_020210690.1 type IV pilus twitching motility protein PilT Machinery gene
  NHM04_RS12395 (NHM04_12395) pilU 2822135..2823280 (+) 1146 WP_254264101.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NHM04_RS12400 (NHM04_12400) - 2823573..2825279 (+) 1707 WP_254264102.1 ShlB/FhaC/HecB family hemolysin secretion/activation protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42398.79 Da        Isoelectric Point: 6.5054

>NTDB_id=704702 NHM04_RS12395 WP_254264101.1 2822135..2823280(+) (pilU) [Gilvimarinus sp. DA14]
MDFDRLLQLMVDKGASDLFITAGVPPSIKLHGKVVPATATPLAPEKARELVLSVMNEKQRREFLEAKELNFAISARGIGR
FRASAFYQRNLAGMVLRRIETKIPKIDDLHLPEVIKELAMTKRGLVMFVGATGTGKSTSLASMIGHRNRNSKGHIISIED
PIEFIHQHEGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEVRSRETMEHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHRQLWMDLSLNLRGIVAQQLIPTPDGKGRRACLEVLLNTPLMSDLIRKGEVGELKELMKKSTELGMQTFDQA
LYNLYDSGEITYEDALAHADSPNDLRLMIKLGSETDATYLSNAADSLSIEEEDTGRGGRMF

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=704702 NHM04_RS12395 WP_254264101.1 2822135..2823280(+) (pilU) [Gilvimarinus sp. DA14]
ATGGATTTTGATCGTCTACTGCAGTTGATGGTGGATAAGGGGGCATCGGACTTATTTATTACCGCCGGTGTGCCACCGTC
AATCAAATTACACGGTAAAGTAGTTCCTGCCACCGCCACACCACTGGCGCCGGAAAAAGCGCGCGAGTTGGTGTTGTCGG
TTATGAATGAAAAGCAGCGCCGGGAATTTCTGGAGGCAAAAGAACTCAACTTTGCCATCAGTGCGCGGGGCATTGGTCGT
TTTCGTGCCAGTGCTTTTTATCAGCGCAACTTGGCGGGCATGGTGCTGCGCCGGATCGAAACCAAAATTCCCAAAATTGA
TGACCTGCATTTGCCTGAGGTGATTAAAGAGCTGGCCATGACCAAGCGCGGCCTGGTGATGTTTGTGGGCGCAACGGGTA
CCGGTAAATCCACCTCGCTGGCGTCGATGATTGGCCATCGCAATCGCAATTCAAAAGGGCATATTATTTCTATCGAAGAC
CCAATTGAATTTATCCACCAGCATGAAGGCTGTATTATTACCCAGCGCGAGGTGGGCATTGATACCGACTCCTTTGAAGT
CGCGCTAAAAAATACCTTGCGCCAGGCGCCGGATGTTATTTTGATTGGCGAGGTGCGCTCGCGCGAAACCATGGAGCACG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCTTATGCACCTTGCACGCTAACAACGCCAACCAGGCGCTGGACCGGATT
ATTCACTTTTTCCCTGCCGACCGTCACCGCCAGTTGTGGATGGACTTATCCCTTAACCTCAGAGGCATTGTGGCGCAGCA
ACTAATACCCACGCCAGATGGCAAAGGCCGCCGCGCTTGTTTGGAAGTGCTGCTCAATACGCCGTTAATGTCTGACTTAA
TTCGCAAAGGTGAAGTGGGTGAGCTAAAAGAGCTAATGAAAAAATCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTGTACGACAGCGGCGAAATCACCTACGAAGACGCACTCGCCCACGCCGACTCGCCCAATGACTTGCGCCT
GATGATTAAGCTCGGCTCCGAAACCGACGCAACCTATCTGTCCAACGCTGCCGACAGCCTCTCCATTGAAGAGGAAGATA
CAGGCCGCGGCGGACGGATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(40-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

77.69

100

0.777
  pilU Acinetobacter baylyi ADP1

68.067

93.701

0.638
  pilU Vibrio cholerae strain A1552

54.301

97.638

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.559

100

0.428
  pilT Pseudomonas aeruginosa PAK

40

90.551

0.362