Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL335_RS11600 Genome accession   NZ_AP018718
Coordinates   2313297..2314340 (-) Length   347 a.a.
NCBI ID   WP_126447071.1    Uniprot ID   -
Organism   Sulfuricystis multivorans strain J5B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2308297..2319340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL335_RS11575 - 2309118..2310002 (+) 885 WP_126447061.1 NAD-dependent epimerase/dehydratase family protein -
  EL335_RS11580 - 2309990..2310406 (+) 417 WP_126447063.1 CopD family protein -
  EL335_RS11585 - 2310418..2311641 (+) 1224 WP_126447065.1 THUMP domain-containing protein -
  EL335_RS11590 - 2311645..2312133 (-) 489 WP_126447067.1 hypothetical protein -
  EL335_RS11595 pilU 2312155..2313291 (-) 1137 WP_126447069.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL335_RS11600 pilT 2313297..2314340 (-) 1044 WP_126447071.1 type IV pilus twitching motility protein PilT Machinery gene
  EL335_RS11605 - 2314408..2315100 (+) 693 WP_126447073.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL335_RS11610 proC 2315097..2315909 (+) 813 WP_126447075.1 pyrroline-5-carboxylate reductase -
  EL335_RS11615 - 2315909..2316454 (+) 546 WP_126447077.1 YggT family protein -
  EL335_RS11620 - 2316454..2316747 (+) 294 WP_126447079.1 DUF167 family protein -
  EL335_RS11625 - 2316750..2317586 (+) 837 WP_126447081.1 symmetrical bis(5'-nucleosyl)-tetraphosphatase -
  EL335_RS11630 ychF 2317588..2318682 (-) 1095 WP_126447083.1 redox-regulated ATPase YchF -
  EL335_RS11635 pth 2318679..2319296 (-) 618 WP_284155356.1 aminoacyl-tRNA hydrolase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38168.98 Da        Isoelectric Point: 7.1068

>NTDB_id=70459 EL335_RS11600 WP_126447071.1 2313297..2314340(-) (pilT) [Sulfuricystis multivorans strain J5B]
MDITELLAFAVKNKASDLHLSSGLPPMIRVHGDIRRINLPPMEHKDVHAMVYDIMNDAQRKHYEETLECDFSFAVPNLAR
FRVNAYVQNRGAAAAFRTIPSKVLTLEQLNAPKIFAEIADQPRGIVLVTGPTGSGKSTTLAAMIDHINEKEYAHILTVED
PIEFVHESKKCLINQREVGPHTLSFANALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVIAQTLLKTKDGNGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQAFGMQTLDQC
LADLVKRNIVSAAEARAKAANKDSFLG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=70459 EL335_RS11600 WP_126447071.1 2313297..2314340(-) (pilT) [Sulfuricystis multivorans strain J5B]
ATGGACATTACCGAATTGCTGGCGTTTGCCGTCAAGAACAAGGCCTCGGACCTGCACTTGTCTTCGGGACTGCCGCCAAT
GATCCGCGTGCATGGCGACATCCGGCGCATCAACCTGCCGCCGATGGAGCACAAGGATGTGCATGCGATGGTCTATGACA
TCATGAACGATGCCCAGCGTAAGCACTACGAAGAGACGCTGGAATGCGATTTCTCGTTCGCGGTGCCGAATCTGGCGCGC
TTCCGCGTCAATGCCTATGTGCAGAACCGCGGTGCAGCGGCGGCGTTCCGTACCATTCCATCGAAGGTGCTCACCCTCGA
ACAGCTCAACGCGCCGAAGATTTTCGCCGAGATTGCCGACCAGCCGCGCGGCATCGTGCTGGTTACTGGGCCCACCGGCT
CCGGCAAATCGACGACGCTGGCGGCAATGATCGATCACATCAACGAAAAGGAATATGCTCACATCCTCACCGTCGAAGAT
CCGATCGAATTCGTCCATGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTTGGCCCGCACACGTTGTCATTCGCCAA
TGCCTTGCGTTCGGCCTTGCGTGAGGATCCGGACGTGATCCTCGTCGGCGAGATGCGCGATCTGGAAACGATCCGTCTGG
CGCTCACTGCCGCCGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGATC
GTCGACGTCTTCCCCGCGGCGGAAAAGGAGATGGTGCGTTCGATGTTGTCCGAATCCTTGCGCGCGGTGATCGCGCAGAC
GCTGTTGAAGACCAAGGACGGCAATGGTCGGGTCGCCGCACACGAGATCATGATCGGCACACCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCCAGGCGTTTGGCATGCAGACCCTCGACCAGTGT
CTGGCGGATCTGGTCAAGCGCAACATCGTCTCGGCGGCCGAGGCGCGCGCCAAAGCCGCCAACAAGGATAGTTTCCTCGG
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.457

99.712

0.772
  pilT Acinetobacter baumannii D1279779

77.457

99.712

0.772
  pilT Acinetobacter baumannii strain A118

77.457

99.712

0.772
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Legionella pneumophila strain Lp02

73.333

99.424

0.729
  pilT Legionella pneumophila strain ERS1305867

73.333

99.424

0.729
  pilT Neisseria meningitidis 8013

72.754

99.424

0.723
  pilT Neisseria gonorrhoeae MS11

72.464

99.424

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.935

97.406

0.671
  pilT Vibrio cholerae strain A1552

68.935

97.406

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Acinetobacter baylyi ADP1

41.124

97.406

0.401
  pilU Vibrio cholerae strain A1552

41.194

96.542

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392

Multiple sequence alignment