Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL335_RS11595 Genome accession   NZ_AP018718
Coordinates   2312155..2313291 (-) Length   378 a.a.
NCBI ID   WP_126447069.1    Uniprot ID   -
Organism   Sulfuricystis multivorans strain J5B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2307155..2318291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL335_RS11570 - 2308099..2309103 (-) 1005 WP_126447059.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  EL335_RS11575 - 2309118..2310002 (+) 885 WP_126447061.1 NAD-dependent epimerase/dehydratase family protein -
  EL335_RS11580 - 2309990..2310406 (+) 417 WP_126447063.1 CopD family protein -
  EL335_RS11585 - 2310418..2311641 (+) 1224 WP_126447065.1 THUMP domain-containing protein -
  EL335_RS11590 - 2311645..2312133 (-) 489 WP_126447067.1 hypothetical protein -
  EL335_RS11595 pilU 2312155..2313291 (-) 1137 WP_126447069.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL335_RS11600 pilT 2313297..2314340 (-) 1044 WP_126447071.1 type IV pilus twitching motility protein PilT Machinery gene
  EL335_RS11605 - 2314408..2315100 (+) 693 WP_126447073.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL335_RS11610 proC 2315097..2315909 (+) 813 WP_126447075.1 pyrroline-5-carboxylate reductase -
  EL335_RS11615 - 2315909..2316454 (+) 546 WP_126447077.1 YggT family protein -
  EL335_RS11620 - 2316454..2316747 (+) 294 WP_126447079.1 DUF167 family protein -
  EL335_RS11625 - 2316750..2317586 (+) 837 WP_126447081.1 symmetrical bis(5'-nucleosyl)-tetraphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42045.56 Da        Isoelectric Point: 6.4349

>NTDB_id=70458 EL335_RS11595 WP_126447069.1 2312155..2313291(-) (pilU) [Sulfuricystis multivorans strain J5B]
MERDESLKFLYQLLAMLVQKKGSDLFITAGFPPAIKVDGKVTPVTAQPLMPQHTIELAHAVMNDKQAATFEETKECNFAI
SPAGIGRFRVNAFIQQGRVGLVFRTINTAIPKFEDLGLPPVLKDIGMTKRGLVLFVGGTGSGKSTSLAAMIGYRNEHSYG
HIITIEDPIEYVHEHKNCIITQREVGVDTDSWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANNA
NQAMDRIINFFPEDRRPQLLMDLSLNLKAVVSQRLLPAREGKGRVAAVEILINSPLISDLIGKGEVHEIKEVMKRSRELG
MQTFDQALFDLYEAGKISYEDALRFADSMNEVRLQIKLHGKEMAERDLASGIKNLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=70458 EL335_RS11595 WP_126447069.1 2312155..2313291(-) (pilU) [Sulfuricystis multivorans strain J5B]
ATGGAACGCGACGAATCGCTCAAATTCCTATACCAGCTCTTGGCGATGCTGGTGCAGAAGAAGGGTTCGGACCTGTTCAT
CACCGCCGGCTTTCCGCCGGCGATCAAGGTCGACGGCAAGGTGACGCCGGTCACCGCGCAGCCGCTGATGCCCCAGCACA
CGATCGAGCTGGCGCACGCGGTGATGAACGACAAGCAGGCGGCGACTTTCGAAGAAACCAAGGAGTGCAATTTCGCGATC
TCGCCTGCCGGCATCGGCCGCTTCCGCGTCAATGCCTTCATCCAGCAGGGGCGCGTCGGCCTGGTGTTCCGCACCATCAA
CACGGCGATCCCGAAATTCGAGGACCTCGGCTTGCCGCCCGTCCTCAAGGACATCGGCATGACCAAGCGCGGTCTGGTGC
TGTTCGTCGGTGGCACCGGCTCGGGCAAATCGACTTCGCTCGCGGCGATGATCGGCTACCGTAACGAACACAGCTACGGC
CACATCATCACGATCGAAGACCCGATCGAATACGTGCATGAGCACAAGAACTGCATCATTACCCAGCGCGAGGTCGGTGT
CGATACCGATTCCTGGGAAGCGGCGCTGAAGAATACGTTGCGTCAGGCGCCCGACGTGATCCTGATCGGCGAGATCCGCG
ATCGGGAGACGATGGAGCACGCGATCGCCTTCGCCGAGACCGGCCATCTGTGCATGGGGACACTGCACGCGAACAATGCC
AACCAGGCGATGGATCGCATCATCAACTTCTTTCCCGAGGATCGGCGGCCACAGCTGTTGATGGATCTGTCATTGAATCT
GAAGGCCGTCGTCTCGCAGCGGCTGCTACCGGCACGCGAAGGCAAGGGGCGCGTCGCGGCGGTCGAGATCCTGATCAACT
CGCCGCTGATCTCCGATCTGATCGGCAAGGGCGAGGTGCACGAGATCAAGGAGGTGATGAAGCGTTCGCGGGAACTCGGC
ATGCAGACATTCGACCAGGCGCTGTTCGATCTCTATGAAGCCGGCAAGATCAGCTACGAGGATGCACTGCGCTTCGCCGA
CTCGATGAACGAAGTGCGCCTGCAGATCAAGCTGCACGGCAAGGAAATGGCCGAGCGCGATCTCGCCAGCGGCATCAAGA
ATCTCGACATCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.131

94.974

0.638
  pilU Acinetobacter baylyi ADP1

61.96

91.799

0.569
  pilU Vibrio cholerae strain A1552

58.046

92.063

0.534
  pilT Acinetobacter baylyi ADP1

46.018

89.683

0.413
  pilT Legionella pneumophila strain Lp02

46.526

87.566

0.407
  pilT Legionella pneumophila strain ERS1305867

46.526

87.566

0.407
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Acinetobacter nosocomialis M2

45.946

88.095

0.405
  pilT Acinetobacter baumannii D1279779

45.946

88.095

0.405
  pilT Acinetobacter baumannii strain A118

45.946

88.095

0.405
  pilT Pseudomonas aeruginosa PAK

45.646

88.095

0.402
  pilT Pseudomonas stutzeri DSM 10701

45.345

88.095

0.399
  pilT Neisseria meningitidis 8013

42.353

89.947

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.072

87.831

0.378
  pilT Vibrio cholerae strain A1552

43.072

87.831

0.378
  pilT Neisseria gonorrhoeae MS11

41.888

89.683

0.376

Multiple sequence alignment