Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VCA1004_RS08875 Genome accession   NZ_AP018689
Coordinates   1948242..1949270 (-) Length   342 a.a.
NCBI ID   WP_086981437.1    Uniprot ID   -
Organism   Vibrio aphrogenes strain CA-1004     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1943242..1954270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCA1004_RS08845 rsmE 1943456..1944187 (+) 732 WP_086981431.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VCA1004_RS08850 gshB 1944245..1945195 (+) 951 WP_086981432.1 glutathione synthase -
  VCA1004_RS08855 - 1945335..1945898 (+) 564 WP_086981433.1 YqgE/AlgH family protein -
  VCA1004_RS08860 ruvX 1945940..1946365 (+) 426 WP_086981434.1 Holliday junction resolvase RuvX -
  VCA1004_RS08865 - 1946634..1947062 (-) 429 WP_086981435.1 DUF3293 domain-containing protein -
  VCA1004_RS08870 pilU 1947103..1948209 (-) 1107 WP_086981436.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VCA1004_RS08875 pilT 1948242..1949270 (-) 1029 WP_086981437.1 type IV pilus twitching motility protein PilT Machinery gene
  VCA1004_RS08880 - 1949296..1950015 (+) 720 WP_086981438.1 YggS family pyridoxal phosphate-dependent enzyme -
  VCA1004_RS08885 proC 1950141..1950959 (+) 819 WP_086981439.1 pyrroline-5-carboxylate reductase -
  VCA1004_RS08890 - 1950989..1951543 (+) 555 WP_086981440.1 YggT family protein -
  VCA1004_RS08895 yggU 1951565..1951861 (+) 297 WP_086981441.1 DUF167 family protein YggU -
  VCA1004_RS08900 - 1951873..1952322 (+) 450 WP_086981442.1 DUF4426 domain-containing protein -
  VCA1004_RS08905 - 1952410..1953018 (+) 609 WP_086981443.1 XTP/dITP diphosphatase -
  VCA1004_RS08910 hemW 1953028..1954197 (+) 1170 WP_086981444.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 38031.54 Da        Isoelectric Point: 5.5932

>NTDB_id=70404 VCA1004_RS08875 WP_086981437.1 1948242..1949270(-) (pilT) [Vibrio aphrogenes strain CA-1004]
MDIAELLDFSVKHNASDLHLSAGVPPMLRIDGDVRKLEMDAFTHAEVHALVFDIMNDKQRSEFEEKLEIDFSFELPEIGR
FRVNAFNQSRGAAAVFRTIPNTIPTFEDIDAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINRNHKKHILTIED
PIEFVHSSNKCLVNQREVHRDTHSFEASLRSALREDPDVILVGELRDQETIALALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGADKDMVRSMLSESLRAVIAQKLFKRNDKGRIACHEVMIATPAIRNLIREDKVAQMLSMIQTGSSLGMQTMEQAA
RQLLLQGLVTKEEVEAKIGSIA

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=70404 VCA1004_RS08875 WP_086981437.1 1948242..1949270(-) (pilT) [Vibrio aphrogenes strain CA-1004]
ATGGATATTGCTGAGTTATTGGATTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCAGGCGTTCCGCCGAT
GTTGCGTATTGATGGTGATGTGAGAAAGTTAGAAATGGATGCTTTCACTCATGCTGAGGTACATGCGTTAGTGTTTGATA
TCATGAATGATAAGCAGCGCAGTGAATTTGAAGAAAAATTAGAAATTGATTTCTCATTTGAATTACCTGAGATCGGGCGC
TTTCGTGTTAATGCCTTTAACCAATCTCGCGGGGCGGCGGCAGTTTTTCGCACTATTCCCAATACTATCCCAACTTTTGA
AGATATCGATGCTCCGGAAATCTTTGAAAAAATAGCCAATTATGAAAAAGGTTTAGTATTAGTCACCGGGCCGACTGGCT
CAGGTAAATCGACTACCTTAGCGGCAATGGTCGATTATATTAATCGTAATCATAAAAAGCACATTTTAACGATTGAAGAC
CCCATCGAATTTGTTCATTCGAGTAATAAATGCTTAGTCAATCAGCGTGAAGTGCACCGCGATACCCATAGTTTTGAAGC
GTCGTTACGTTCGGCTTTACGTGAAGATCCCGATGTGATTTTAGTTGGGGAATTGCGCGACCAAGAAACCATTGCATTAG
CTTTAACGGCGGCAGAAACCGGACATTTGGTCTTTGGCACTCTGCATACCAGTTCTGCCGCGAAAACCATTGACCGTATT
ATTGATGTTTTCCCCGGTGCAGATAAAGACATGGTGCGCTCGATGTTATCGGAATCTTTGCGTGCGGTGATTGCACAAAA
ACTATTTAAACGTAACGATAAAGGCCGTATTGCCTGTCATGAAGTAATGATTGCTACGCCTGCGATTCGTAACTTGATCC
GTGAAGATAAAGTGGCACAAATGCTCTCTATGATTCAAACCGGATCGTCGCTTGGCATGCAAACCATGGAGCAAGCTGCA
CGTCAATTATTGTTGCAAGGCTTGGTCACAAAAGAAGAAGTGGAAGCCAAAATCGGAAGTATTGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

81.953

98.83

0.81
  pilT Vibrio cholerae strain A1552

81.953

98.83

0.81
  pilT Pseudomonas stutzeri DSM 10701

69.436

98.538

0.684
  pilT Acinetobacter baumannii strain A118

69.277

97.076

0.673
  pilT Acinetobacter baumannii D1279779

69.277

97.076

0.673
  pilT Acinetobacter nosocomialis M2

69.277

97.076

0.673
  pilT Pseudomonas aeruginosa PAK

68.249

98.538

0.673
  pilT Acinetobacter baylyi ADP1

68.997

96.199

0.664
  pilT Legionella pneumophila strain Lp02

67.372

96.784

0.652
  pilT Legionella pneumophila strain ERS1305867

67.372

96.784

0.652
  pilT Neisseria meningitidis 8013

63.063

97.368

0.614
  pilT Neisseria gonorrhoeae MS11

62.763

97.368

0.611
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.401

97.661

0.482
  pilU Pseudomonas stutzeri DSM 10701

44.012

97.661

0.43
  pilU Vibrio cholerae strain A1552

41.916

97.661

0.409
  pilU Acinetobacter baylyi ADP1

41.745

93.86

0.392

Multiple sequence alignment