Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VCA1004_RS08870 Genome accession   NZ_AP018689
Coordinates   1947103..1948209 (-) Length   368 a.a.
NCBI ID   WP_086981436.1    Uniprot ID   -
Organism   Vibrio aphrogenes strain CA-1004     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1942103..1953209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCA1004_RS08840 - 1942892..1943377 (+) 486 WP_197715832.1 SprT family zinc-dependent metalloprotease -
  VCA1004_RS08845 rsmE 1943456..1944187 (+) 732 WP_086981431.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VCA1004_RS08850 gshB 1944245..1945195 (+) 951 WP_086981432.1 glutathione synthase -
  VCA1004_RS08855 - 1945335..1945898 (+) 564 WP_086981433.1 YqgE/AlgH family protein -
  VCA1004_RS08860 ruvX 1945940..1946365 (+) 426 WP_086981434.1 Holliday junction resolvase RuvX -
  VCA1004_RS08865 - 1946634..1947062 (-) 429 WP_086981435.1 DUF3293 domain-containing protein -
  VCA1004_RS08870 pilU 1947103..1948209 (-) 1107 WP_086981436.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VCA1004_RS08875 pilT 1948242..1949270 (-) 1029 WP_086981437.1 type IV pilus twitching motility protein PilT Machinery gene
  VCA1004_RS08880 - 1949296..1950015 (+) 720 WP_086981438.1 YggS family pyridoxal phosphate-dependent enzyme -
  VCA1004_RS08885 proC 1950141..1950959 (+) 819 WP_086981439.1 pyrroline-5-carboxylate reductase -
  VCA1004_RS08890 - 1950989..1951543 (+) 555 WP_086981440.1 YggT family protein -
  VCA1004_RS08895 yggU 1951565..1951861 (+) 297 WP_086981441.1 DUF167 family protein YggU -
  VCA1004_RS08900 - 1951873..1952322 (+) 450 WP_086981442.1 DUF4426 domain-containing protein -
  VCA1004_RS08905 - 1952410..1953018 (+) 609 WP_086981443.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41248.26 Da        Isoelectric Point: 5.7970

>NTDB_id=70403 VCA1004_RS08870 WP_086981436.1 1947103..1948209(-) (pilU) [Vibrio aphrogenes strain CA-1004]
MQLSQILDIMVSSKASDIYLTVGAPCLFRVNGELKPQGEPLDRHAVEALLFEMMDAERQADYQATHEANFAIVSGSGRFR
VSAFYQRELPGAVLRRIETQIPSMEALNLPEILKSVAVAKRGLVLVVGATGSGKSTSMAAMTGYRNQNQSGHILTVEDPI
EFVHEHQQCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRNRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSLNLKAVIAQQLIRDKEEDKRHGVFEVLLNTPRVSDLIRRGELHELKTTMMRSKESGMQTFDQALF
DLLQQDKISLEDAMHCADSANDLRMMLKSANSGSSFTSNKLKDIKIDL

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=70403 VCA1004_RS08870 WP_086981436.1 1947103..1948209(-) (pilU) [Vibrio aphrogenes strain CA-1004]
ATGCAACTCTCTCAAATATTAGACATTATGGTGTCATCAAAAGCCTCGGATATTTATTTAACCGTTGGAGCGCCTTGTTT
ATTTCGGGTGAATGGTGAGCTGAAACCGCAGGGTGAGCCTTTAGATCGTCATGCCGTTGAAGCGCTATTATTTGAGATGA
TGGACGCTGAGCGCCAAGCTGATTATCAAGCTACTCACGAAGCTAATTTTGCTATTGTCAGTGGCAGTGGGCGTTTTCGT
GTGAGTGCGTTTTATCAGCGAGAATTGCCAGGAGCGGTACTACGACGTATTGAAACTCAGATTCCGAGTATGGAAGCGTT
GAATTTACCTGAAATATTGAAAAGCGTTGCTGTGGCTAAGCGTGGTTTAGTGCTGGTGGTTGGGGCGACGGGCTCTGGAA
AATCTACTTCGATGGCCGCCATGACGGGCTATCGTAACCAGAATCAAAGTGGGCATATTTTAACAGTAGAAGACCCGATT
GAGTTTGTGCATGAACATCAGCAATGTATCGTGACTCAACGTGAAGTAGGTTTAGATACAGAAAGTTATGAAATCGCCTT
AAAAAACTCATTGCGCCAAGCACCGGATATGATCTTAATCGGTGAGATTCGTAATCGTGAAACCATGGAATATGCCATGA
CGTTTGCAGAGACTGGGCATTTATGTATGGCAACATTGCATGCAAATAATGCCAACCAAGCGTTAGAGCGTATTTTACAT
TTAGTTCCTAAAGAGCAAAAAGAACAATTTTTGTTTGATTTGTCTCTAAACTTAAAAGCGGTGATTGCTCAGCAATTAAT
ACGTGATAAAGAAGAAGATAAACGTCATGGGGTGTTTGAGGTGCTATTGAATACGCCAAGGGTTTCGGATTTGATTCGTC
GAGGCGAGTTGCATGAGTTAAAAACCACTATGATGCGTTCCAAAGAATCAGGTATGCAAACTTTCGACCAAGCGCTGTTT
GATTTATTACAGCAAGATAAAATCAGTTTAGAGGATGCCATGCATTGTGCTGATTCTGCCAATGATTTGCGTATGATGTT
GAAATCCGCCAATTCTGGATCAAGCTTTACGAGCAATAAGCTTAAAGATATTAAAATTGATTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

75.272

100

0.753
  pilU Pseudomonas stutzeri DSM 10701

57.182

98.37

0.562
  pilU Acinetobacter baylyi ADP1

53.444

98.641

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

96.467

0.386
  pilT Legionella pneumophila strain Lp02

41.298

92.12

0.38
  pilT Legionella pneumophila strain ERS1305867

41.298

92.12

0.38
  pilT Acinetobacter baylyi ADP1

40.762

92.663

0.378
  pilT Pseudomonas aeruginosa PAK

40.597

91.033

0.37
  pilT Acinetobacter nosocomialis M2

39.823

92.12

0.367
  pilT Acinetobacter baumannii D1279779

39.823

92.12

0.367
  pilT Acinetobacter baumannii strain A118

39.823

92.12

0.367
  pilT Pseudomonas stutzeri DSM 10701

40

91.033

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.693

86.685

0.361
  pilT Vibrio cholerae strain A1552

41.693

86.685

0.361

Multiple sequence alignment