Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VRUMOI_RS03110 Genome accession   NZ_AP018685
Coordinates   682885..683934 (+) Length   349 a.a.
NCBI ID   WP_089137901.1    Uniprot ID   -
Organism   Vibrio rumoiensis strain FERM P-14531     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 677885..688934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VRUMOI_RS03075 hemW 678103..679278 (-) 1176 WP_089137908.1 radical SAM family heme chaperone HemW -
  VRUMOI_RS03080 - 679282..679890 (-) 609 WP_089137907.1 XTP/dITP diphosphatase -
  VRUMOI_RS03085 - 679908..680339 (-) 432 WP_089137906.1 DUF4426 domain-containing protein -
  VRUMOI_RS03090 yggU 680353..680643 (-) 291 WP_089137905.1 DUF167 family protein YggU -
  VRUMOI_RS03095 - 680647..681201 (-) 555 WP_089137904.1 YggT family protein -
  VRUMOI_RS03100 proC 681237..682055 (-) 819 WP_089137903.1 pyrroline-5-carboxylate reductase -
  VRUMOI_RS03105 - 682152..682856 (-) 705 WP_231897500.1 YggS family pyridoxal phosphate-dependent enzyme -
  VRUMOI_RS03110 pilT 682885..683934 (+) 1050 WP_089137901.1 type IV pilus twitching motility protein PilT Machinery gene
  VRUMOI_RS03115 pilU 683950..685056 (+) 1107 WP_089137900.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VRUMOI_RS03120 - 685135..686115 (-) 981 WP_089137899.1 hypothetical protein -
  VRUMOI_RS03125 ruvX 686257..686682 (-) 426 WP_089137898.1 Holliday junction resolvase RuvX -
  VRUMOI_RS03130 - 686716..687279 (-) 564 WP_089137897.1 YqgE/AlgH family protein -
  VRUMOI_RS03135 gshB 687356..688309 (-) 954 WP_089137896.1 glutathione synthase -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38632.23 Da        Isoelectric Point: 5.5880

>NTDB_id=70357 VRUMOI_RS03110 WP_089137901.1 682885..683934(+) (pilT) [Vibrio rumoiensis strain FERM P-14531]
MDIAELLEFSVKHNASDLHLSAGVPPILRIDGDIRQLEMAAFTHSDVHALVFDIMNDAQRSEFEEKLEIDFSFELPNIGR
FRVNVFNQSRGCAAVFRTIPVEIPSFEDISAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINRNHKKHILTIED
PIEFIHSNKKCLVNQREVHRDTHSFEASLRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGTDKNMVRSMLSESLRAVIAQKLLKRGGDAGGRIACHEVMIATPAIRNLVREDKVAQMLSVIQTGSAMGMQTMEQ
AARQLLMQGLVSQEEVESKIETLAPTSPY

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=70357 VRUMOI_RS03110 WP_089137901.1 682885..683934(+) (pilT) [Vibrio rumoiensis strain FERM P-14531]
ATGGATATTGCTGAGTTATTGGAATTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCTGGCGTTCCTCCAAT
CTTACGTATTGATGGCGACATCAGACAGTTGGAAATGGCAGCTTTTACTCATTCAGATGTTCATGCATTAGTCTTTGATA
TTATGAACGATGCCCAGCGTAGCGAATTTGAAGAAAAACTAGAAATCGATTTTTCGTTTGAACTGCCAAATATTGGTCGC
TTTCGTGTCAATGTTTTCAACCAATCTCGCGGCTGTGCGGCTGTATTTCGTACCATTCCGGTAGAAATTCCTTCATTTGA
AGATATTTCAGCACCCGAGATTTTCGAAAAAATTGCTAATTATGAAAAAGGCTTAGTGTTAGTGACTGGCCCAACCGGTT
CAGGTAAATCAACAACCTTAGCGGCGATGGTGGATTACATTAATCGTAATCACAAAAAGCATATTCTGACGATTGAAGAT
CCTATCGAATTTATTCACTCGAATAAAAAATGTTTGGTTAACCAACGCGAAGTTCACCGTGATACTCATAGCTTTGAAGC
GTCTCTACGTTCGGCGTTACGTGAAGACCCTGATGTAATTTTGGTGGGGGAACTACGTGATAAAGAAACCATCAGTTTGG
CATTAACGGCTGCTGAAACTGGTCACTTAGTGTTTGGTACTTTGCATACCAGTTCTGCCGCTAAAACCATTGACCGTATT
ATTGATGTATTCCCAGGCACAGATAAAAACATGGTTCGTTCTATGTTATCGGAGTCTTTGCGTGCGGTGATTGCGCAGAA
GTTATTAAAACGCGGTGGGGATGCCGGTGGGCGTATCGCTTGTCATGAAGTGATGATTGCCACTCCTGCTATTCGTAACC
TGGTTCGTGAAGATAAAGTCGCTCAAATGTTATCGGTTATTCAAACTGGTTCAGCGATGGGGATGCAAACCATGGAGCAA
GCGGCTCGACAGCTACTGATGCAAGGTTTAGTGAGTCAAGAAGAAGTTGAAAGCAAAATTGAAACCCTAGCGCCAACGTC
GCCCTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

82.111

97.708

0.802
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

82.111

97.708

0.802
  pilT Pseudomonas stutzeri DSM 10701

67.257

97.135

0.653
  pilT Acinetobacter baumannii strain A118

67.665

95.702

0.648
  pilT Acinetobacter nosocomialis M2

67.665

95.702

0.648
  pilT Acinetobacter baumannii D1279779

67.665

95.702

0.648
  pilT Acinetobacter baylyi ADP1

67.771

95.129

0.645
  pilT Pseudomonas aeruginosa PAK

65.782

97.135

0.639
  pilT Legionella pneumophila strain Lp02

66.967

95.415

0.639
  pilT Legionella pneumophila strain ERS1305867

66.967

95.415

0.639
  pilT Neisseria meningitidis 8013

62.575

95.702

0.599
  pilT Neisseria gonorrhoeae MS11

62.275

95.702

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.552

95.989

0.476
  pilU Pseudomonas stutzeri DSM 10701

42.687

95.989

0.41
  pilU Vibrio cholerae strain A1552

41.194

95.989

0.395
  pilU Acinetobacter baylyi ADP1

40.994

92.264

0.378

Multiple sequence alignment