Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VRUMOI_RS03115 Genome accession   NZ_AP018685
Coordinates   683950..685056 (+) Length   368 a.a.
NCBI ID   WP_089137900.1    Uniprot ID   -
Organism   Vibrio rumoiensis strain FERM P-14531     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 678950..690056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VRUMOI_RS03080 - 679282..679890 (-) 609 WP_089137907.1 XTP/dITP diphosphatase -
  VRUMOI_RS03085 - 679908..680339 (-) 432 WP_089137906.1 DUF4426 domain-containing protein -
  VRUMOI_RS03090 yggU 680353..680643 (-) 291 WP_089137905.1 DUF167 family protein YggU -
  VRUMOI_RS03095 - 680647..681201 (-) 555 WP_089137904.1 YggT family protein -
  VRUMOI_RS03100 proC 681237..682055 (-) 819 WP_089137903.1 pyrroline-5-carboxylate reductase -
  VRUMOI_RS03105 - 682152..682856 (-) 705 WP_231897500.1 YggS family pyridoxal phosphate-dependent enzyme -
  VRUMOI_RS03110 pilT 682885..683934 (+) 1050 WP_089137901.1 type IV pilus twitching motility protein PilT Machinery gene
  VRUMOI_RS03115 pilU 683950..685056 (+) 1107 WP_089137900.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VRUMOI_RS03120 - 685135..686115 (-) 981 WP_089137899.1 hypothetical protein -
  VRUMOI_RS03125 ruvX 686257..686682 (-) 426 WP_089137898.1 Holliday junction resolvase RuvX -
  VRUMOI_RS03130 - 686716..687279 (-) 564 WP_089137897.1 YqgE/AlgH family protein -
  VRUMOI_RS03135 gshB 687356..688309 (-) 954 WP_089137896.1 glutathione synthase -
  VRUMOI_RS03140 rsmE 688367..689098 (-) 732 WP_089137895.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41002.81 Da        Isoelectric Point: 6.1521

>NTDB_id=70358 VRUMOI_RS03115 WP_089137900.1 683950..685056(+) (pilU) [Vibrio rumoiensis strain FERM P-14531]
MQLSQVLEIMVSSKASDIYLTVGASCLFRIDGELKAHGDPLDKGGVEALLFEMMDNERRQEYQSTHEANFAITHGEGRFR
VSAFFQRELPGAVIRRIETRIPSMEELHLPEVLKELSTAKRGLVLVVGATGSGKSTSMAAMTGYRNLNKTGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYDVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSLNLKGVVAQQLVRDKYGNGRHGVFEVLLNTPRVSDLIRRGELHGLKSTMLKSGESGMQTFDQALF
KLIIEDKITEEDAFHSADSANDLRLMLKSHSSDTSGSSALSGIRIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=70358 VRUMOI_RS03115 WP_089137900.1 683950..685056(+) (pilU) [Vibrio rumoiensis strain FERM P-14531]
ATGCAACTTTCTCAAGTACTCGAAATCATGGTGTCATCCAAAGCGTCGGACATTTATTTGACGGTTGGTGCGTCATGTTT
GTTTCGCATTGATGGTGAGCTTAAAGCACATGGTGATCCATTAGATAAAGGTGGAGTAGAAGCCTTATTATTTGAAATGA
TGGATAACGAAAGACGCCAAGAGTACCAATCTACTCATGAAGCAAATTTTGCCATTACGCATGGAGAAGGGCGTTTTCGT
GTCAGTGCTTTTTTCCAGAGAGAGTTACCAGGGGCGGTTATTCGTCGAATTGAAACACGTATCCCGAGTATGGAAGAGCT
ACATTTACCTGAAGTGCTAAAAGAACTGTCGACAGCAAAGCGTGGTTTAGTGCTGGTGGTCGGTGCTACGGGGTCAGGTA
AATCGACGTCGATGGCGGCGATGACGGGTTATCGTAACTTAAACAAAACTGGTCATATATTGACAGTGGAAGATCCGATA
GAGTTTGTTCATGAACACAAAAAATGTATTGTTACTCAGCGTGAAGTTGGCCTAGATACAGAAAGTTATGATGTGGCACT
GAAGAACTCTTTACGCCAAGCACCAGACATGATTTTAATTGGTGAGATCCGTAGCCGAGAAACTATGGAATATGCTATGA
CGTTTGCTGAAACTGGTCACTTGTGTATGGCAACCTTGCATGCCAATAATGCTAACCAAGCGTTAGAGCGCATTTTGCAC
TTAGTGCCGAAAGAGCAAAAAGAACAATTCTTGTTTGATTTATCTTTGAACCTGAAAGGGGTGGTTGCCCAGCAACTTGT
ACGAGATAAGTATGGCAATGGTCGCCATGGTGTTTTTGAAGTGCTACTTAATACGCCACGCGTGTCAGATTTAATCCGTC
GTGGCGAATTGCACGGGCTAAAATCGACGATGCTAAAATCGGGTGAAAGTGGCATGCAAACCTTTGACCAAGCACTATTT
AAGTTGATCATCGAAGACAAGATCACCGAAGAGGACGCGTTCCACAGTGCCGACTCGGCCAACGATCTACGCTTGATGCT
GAAGTCGCACAGCAGCGACACCAGTGGCAGCAGTGCACTCAGCGGTATACGTATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

76.359

100

0.764
  pilU Pseudomonas stutzeri DSM 10701

55.645

100

0.562
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.274

98.098

0.405
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Vibrio cholerae strain A1552

42.462

88.315

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.462

88.315

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria meningitidis 8013

38.841

93.75

0.364
  pilT Neisseria gonorrhoeae MS11

38.841

93.75

0.364
  pilT Acinetobacter nosocomialis M2

39.528

92.12

0.364
  pilT Acinetobacter baumannii D1279779

39.528

92.12

0.364
  pilT Acinetobacter baumannii strain A118

39.528

92.12

0.364

Multiple sequence alignment