Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PCS83_RS20585 Genome accession   NZ_CP115320
Coordinates   4191371..4192804 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain CUVET18-565     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4186371..4197804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS83_RS20555 (PCS83_20495) yrbG 4186929..4187906 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  PCS83_RS20560 (PCS83_20500) kdsD 4187920..4188906 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PCS83_RS20565 (PCS83_20505) kdsC 4188927..4189493 (+) 567 WP_000030015.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PCS83_RS20570 (PCS83_20510) lptC 4189490..4190065 (+) 576 WP_001539833.1 LPS export ABC transporter periplasmic protein LptC -
  PCS83_RS20575 (PCS83_20515) lptA 4190034..4190591 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PCS83_RS20580 (PCS83_20520) lptB 4190598..4191323 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PCS83_RS20585 (PCS83_20525) rpoN/rpoN1 4191371..4192804 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  PCS83_RS20590 (PCS83_20530) hpf 4192827..4193114 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PCS83_RS20595 (PCS83_20535) ptsN 4193232..4193723 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PCS83_RS20600 (PCS83_20540) rapZ 4193769..4194623 (+) 855 WP_000243741.1 RNase adapter RapZ -
  PCS83_RS20605 (PCS83_20545) npr 4194620..4194892 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PCS83_RS20610 (PCS83_20550) yrbL 4195106..4195738 (+) 633 WP_001539838.1 PhoP regulatory network protein YrbL -
  PCS83_RS20615 (PCS83_20555) mtgA 4195735..4196463 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PCS83_RS20620 (PCS83_20560) elbB 4196460..4197113 (-) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=703217 PCS83_RS20585 WP_000809051.1 4191371..4192804(+) (rpoN/rpoN1) [Escherichia coli strain CUVET18-565]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=703217 PCS83_RS20585 WP_000809051.1 4191371..4192804(+) (rpoN/rpoN1) [Escherichia coli strain CUVET18-565]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGATACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAAAGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465