Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NIH92_RS02680 Genome accession   NZ_CP099989
Coordinates   534872..535990 (+) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain KBN10P04593     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 506509..551245 534872..535990 within 0


Gene organization within MGE regions


Location: 506509..551245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIH92_RS02505 (NIH92_02505) - 506775..507230 (-) 456 WP_000575725.1 hypothetical protein -
  NIH92_RS02510 (NIH92_02510) - 507227..507412 (-) 186 WP_000200037.1 hypothetical protein -
  NIH92_RS02515 (NIH92_02515) - 507409..507609 (-) 201 WP_000290738.1 hypothetical protein -
  NIH92_RS02520 (NIH92_02520) - 507643..508017 (-) 375 WP_000818521.1 hypothetical protein -
  NIH92_RS02525 (NIH92_02525) - 508050..508283 (-) 234 WP_000335919.1 helix-turn-helix transcriptional regulator -
  NIH92_RS02530 (NIH92_02530) - 508410..509099 (+) 690 WP_000550612.1 S24 family peptidase -
  NIH92_RS02535 (NIH92_02535) - 509111..509386 (+) 276 WP_000922095.1 hypothetical protein -
  NIH92_RS02540 (NIH92_02540) - 509602..509934 (+) 333 WP_000105905.1 hypothetical protein -
  NIH92_RS02545 (NIH92_02545) - 509974..510777 (+) 804 WP_001290757.1 DUF2303 family protein -
  NIH92_RS02550 (NIH92_02550) - 510863..511123 (+) 261 WP_000369787.1 hypothetical protein -
  NIH92_RS02555 (NIH92_02555) - 511182..511511 (+) 330 WP_001072363.1 hypothetical protein -
  NIH92_RS02560 (NIH92_02560) - 511508..511879 (+) 372 WP_001260061.1 hypothetical protein -
  NIH92_RS02565 (NIH92_02565) - 512050..512730 (+) 681 WP_070419661.1 3'-5' exonuclease -
  NIH92_RS02570 (NIH92_02570) - 512743..513042 (+) 300 Protein_506 ead/Ea22-like family protein -
  NIH92_RS02575 (NIH92_02575) - 513042..513221 (+) 180 Protein_507 hypothetical protein -
  NIH92_RS02580 (NIH92_02580) - 513232..513402 (+) 171 WP_000119263.1 hypothetical protein -
  NIH92_RS02585 (NIH92_02585) - 513413..514081 (-) 669 WP_020752531.1 hypothetical protein -
  NIH92_RS02590 (NIH92_02590) - 514081..514605 (-) 525 Protein_510 site-specific integrase -
  NIH92_RS02595 (NIH92_02595) - 514660..515592 (-) 933 WP_002048759.1 IS5 family transposase -
  NIH92_RS02600 (NIH92_02600) - 515648..516280 (-) 633 Protein_512 Arm DNA-binding domain-containing protein -
  NIH92_RS02610 (NIH92_02610) ggt 516978..518963 (-) 1986 WP_001042923.1 gamma-glutamyltransferase -
  NIH92_RS02615 (NIH92_02615) - 519266..520789 (-) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  NIH92_RS02620 (NIH92_02620) - 520796..521947 (-) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  NIH92_RS02625 (NIH92_02625) - 522217..522714 (-) 498 WP_002019577.1 thioesterase family protein -
  NIH92_RS02630 (NIH92_02630) hemB 522857..523870 (+) 1014 WP_000222569.1 porphobilinogen synthase -
  NIH92_RS02635 (NIH92_02635) - 523896..525011 (+) 1116 WP_000546701.1 FAD-dependent oxidoreductase -
  NIH92_RS02640 (NIH92_02640) - 525003..525908 (-) 906 WP_001039356.1 TIGR01777 family oxidoreductase -
  NIH92_RS02645 (NIH92_02645) - 525998..526450 (-) 453 WP_000164153.1 ABZJ_00895 family protein -
  NIH92_RS02650 (NIH92_02650) - 526830..527207 (+) 378 WP_001059438.1 VOC family protein -
  NIH92_RS02655 (NIH92_02655) - 527275..527748 (-) 474 WP_000974358.1 OsmC family protein -
  NIH92_RS02660 (NIH92_02660) - 527912..529180 (+) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  NIH92_RS02665 (NIH92_02665) - 529190..532786 (+) 3597 WP_000698785.1 SbcC/MukB-like Walker B domain-containing protein -
  NIH92_RS02670 (NIH92_02670) - 532988..533680 (-) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  NIH92_RS02675 (NIH92_02675) pilT 533807..534844 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  NIH92_RS02680 (NIH92_02680) pilU 534872..535990 (+) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NIH92_RS02685 (NIH92_02685) fur 536060..536497 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  NIH92_RS02690 (NIH92_02690) bamE 536609..537007 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  NIH92_RS02695 (NIH92_02695) - 537062..537385 (-) 324 WP_000442587.1 RnfH family protein -
  NIH92_RS02700 (NIH92_02700) - 537385..538452 (-) 1068 WP_002019572.1 hypothetical protein -
  NIH92_RS02705 (NIH92_02705) - 538605..539060 (-) 456 WP_000782976.1 bacteriohemerythrin -
  NIH92_RS02710 (NIH92_02710) - 539313..540161 (-) 849 WP_000336548.1 class II glutamine amidotransferase -
  NIH92_RS02715 (NIH92_02715) - 540270..541052 (-) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  NIH92_RS02720 (NIH92_02720) argB 541173..542081 (-) 909 WP_001135419.1 acetylglutamate kinase -
  NIH92_RS02725 (NIH92_02725) - 542096..543514 (-) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  NIH92_RS02730 (NIH92_02730) dut 543714..544166 (-) 453 WP_000868152.1 dUTP diphosphatase -
  NIH92_RS02735 (NIH92_02735) rep 544191..546230 (-) 2040 WP_000093035.1 DNA helicase Rep -
  NIH92_RS02740 (NIH92_02740) - 546424..547077 (-) 654 WP_001202415.1 OmpA family protein -
  NIH92_RS02745 (NIH92_02745) - 547322..548248 (+) 927 WP_000100965.1 acyltransferase -
  NIH92_RS02750 (NIH92_02750) - 548265..549311 (+) 1047 WP_001181639.1 hypothetical protein -
  NIH92_RS02755 (NIH92_02755) minC 549433..550155 (+) 723 WP_000763677.1 septum site-determining protein MinC -
  NIH92_RS02760 (NIH92_02760) minD 550226..551038 (+) 813 WP_001074362.1 septum site-determining protein MinD -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=703218 NIH92_RS02680 WP_000347039.1 534872..535990(+) (pilU) [Acinetobacter baumannii strain KBN10P04593]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=703218 NIH92_RS02680 WP_000347039.1 534872..535990(+) (pilU) [Acinetobacter baumannii strain KBN10P04593]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395