Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NI376_RS03280 Genome accession   NZ_CP099943
Coordinates   703148..704194 (-) Length   348 a.a.
NCBI ID   WP_130126814.1    Uniprot ID   -
Organism   Pseudoalteromonas piscicida strain BM7A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 698148..709194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI376_RS03255 (NI376_03250) gshB 698803..699763 (+) 961 Protein_630 glutathione synthase -
  NI376_RS03260 (NI376_03255) - 699783..700340 (+) 558 WP_010379034.1 YqgE/AlgH family protein -
  NI376_RS03265 (NI376_03260) ruvX 700383..700832 (+) 450 WP_017217006.1 Holliday junction resolvase RuvX -
  NI376_RS03270 (NI376_03265) - 701147..702034 (-) 888 WP_308374155.1 hypothetical protein -
  NI376_RS03275 (NI376_03270) pilU 702012..703133 (-) 1122 WP_010607713.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NI376_RS03280 (NI376_03275) pilT 703148..704194 (-) 1047 WP_130126814.1 type IV pilus twitching motility protein PilT Machinery gene
  NI376_RS03285 (NI376_03280) - 704225..704908 (+) 684 WP_308374157.1 YggS family pyridoxal phosphate-dependent enzyme -
  NI376_RS03290 (NI376_03285) proC 704922..705743 (+) 822 WP_308374158.1 pyrroline-5-carboxylate reductase -
  NI376_RS03295 (NI376_03290) - 705753..706286 (+) 534 WP_010379021.1 YggT family protein -
  NI376_RS03300 (NI376_03295) - 706297..706731 (+) 435 WP_308374160.1 DUF4426 domain-containing protein -
  NI376_RS03305 (NI376_03300) - 706740..707333 (+) 594 WP_308374161.1 XTP/dITP diphosphatase -
  NI376_RS03310 (NI376_03305) hemW 707330..708466 (+) 1137 WP_308374162.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38649.92 Da        Isoelectric Point: 6.3154

>NTDB_id=702757 NI376_RS03280 WP_130126814.1 703148..704194(-) (pilT) [Pseudoalteromonas piscicida strain BM7A]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRVNIPPLEVKDVSSLIYDIMNDNQRRDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPNQVLSLDDLGAPDIFTKISDNPRGLVLVTGPTGSGKSTTLAAMVDYINNNKHHHILTIED
PIEFVHDNKQSLINQREVHRDTHSFNAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGEEKDMVRSMLSESLQAVISQTLVKKVGGGRIAAHEIMLGIPAIRNLIREDKIAQMYSAIQTGAMHGMQTMDQCL
TNLLNRGLITQQDARAKAHDKNQFNSGY

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=702757 NI376_RS03280 WP_130126814.1 703148..704194(-) (pilT) [Pseudoalteromonas piscicida strain BM7A]
ATGGATATTACAGAATTATTGGCTTTTAGTGTTCAACACAAAGCATCTGACTTACACCTTTCTTCAGGTGTTTCCCCTAT
GATCCGTGTTGACGGGGACGTACGCCGGGTGAATATTCCGCCGTTAGAAGTAAAAGACGTCAGCAGCCTCATCTACGATA
TTATGAATGATAATCAACGTCGTGACTATGAGCAAAACCTAGAGGTAGATTTCTCGTTCGAAGTACCAAATCTGGCGCGT
TTTCGTGTTAACGCCTTTAATTCTAATCGTGGTCCTGCTGCGGTGTTCCGTACCATTCCAAATCAAGTGCTGAGCTTAGA
TGATTTGGGGGCACCAGACATTTTTACCAAAATTTCCGACAACCCACGTGGCTTAGTATTGGTAACCGGCCCGACAGGCT
CTGGTAAATCAACCACGCTGGCTGCGATGGTGGATTATATTAATAACAACAAACATCACCATATTCTAACCATCGAAGAC
CCGATTGAATTTGTTCACGACAACAAGCAAAGCCTTATTAACCAACGTGAAGTACACAGAGACACGCACAGCTTTAACGC
GGCGCTGAGAAGTGCACTCCGTGAAGACCCAGACGTTATTTTGGTCGGTGAATTACGTGATCTTGAGACCATTCGTTTAG
CGCTAACTGCCGCGGAAACAGGTCACTTAGTATTTGGTACTTTGCATACTACCTCGGCACCTAAAACCATCGACCGTATT
GTGGACGTGTTCCCAGGTGAAGAAAAAGACATGGTGCGTTCTATGCTGTCAGAGTCGCTACAAGCGGTGATTTCACAAAC
GTTGGTGAAAAAAGTTGGCGGCGGGCGCATAGCAGCGCATGAAATCATGCTCGGGATCCCAGCTATTCGTAACCTTATTC
GTGAAGACAAGATTGCACAGATGTATTCGGCAATTCAAACCGGTGCAATGCACGGTATGCAAACTATGGATCAATGCCTT
ACAAATCTATTAAACCGTGGTTTGATCACACAGCAGGATGCGCGTGCAAAAGCGCATGACAAAAATCAGTTTAATAGCGG
CTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.035

98.851

0.761
  pilT Acinetobacter baumannii D1279779

77.035

98.851

0.761
  pilT Acinetobacter baumannii strain A118

77.035

98.851

0.761
  pilT Acinetobacter baylyi ADP1

76.744

98.851

0.759
  pilT Pseudomonas stutzeri DSM 10701

75.872

98.851

0.75
  pilT Pseudomonas aeruginosa PAK

75.581

98.851

0.747
  pilT Legionella pneumophila strain Lp02

71.221

98.851

0.704
  pilT Legionella pneumophila strain ERS1305867

71.221

98.851

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

96.839

0.69
  pilT Vibrio cholerae strain A1552

71.217

96.839

0.69
  pilT Neisseria meningitidis 8013

69.208

97.989

0.678
  pilT Neisseria gonorrhoeae MS11

68.915

97.989

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

99.138

0.523
  pilU Vibrio cholerae strain A1552

40.525

98.563

0.399
  pilU Pseudomonas stutzeri DSM 10701

39.942

98.563

0.394
  pilU Acinetobacter baylyi ADP1

38.953

98.851

0.385