Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NI376_RS03275 Genome accession   NZ_CP099943
Coordinates   702012..703133 (-) Length   373 a.a.
NCBI ID   WP_010607713.1    Uniprot ID   A0A2K9JUM3
Organism   Pseudoalteromonas piscicida strain BM7A     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697012..708133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI376_RS03245 (NI376_03240) - 697451..697948 (+) 498 WP_039494456.1 hypothetical protein -
  NI376_RS03250 (NI376_03245) rsmE 698011..698745 (+) 735 WP_010379040.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NI376_RS03255 (NI376_03250) gshB 698803..699763 (+) 961 Protein_630 glutathione synthase -
  NI376_RS03260 (NI376_03255) - 699783..700340 (+) 558 WP_010379034.1 YqgE/AlgH family protein -
  NI376_RS03265 (NI376_03260) ruvX 700383..700832 (+) 450 WP_017217006.1 Holliday junction resolvase RuvX -
  NI376_RS03270 (NI376_03265) - 701147..702034 (-) 888 WP_308374155.1 hypothetical protein -
  NI376_RS03275 (NI376_03270) pilU 702012..703133 (-) 1122 WP_010607713.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NI376_RS03280 (NI376_03275) pilT 703148..704194 (-) 1047 WP_130126814.1 type IV pilus twitching motility protein PilT Machinery gene
  NI376_RS03285 (NI376_03280) - 704225..704908 (+) 684 WP_308374157.1 YggS family pyridoxal phosphate-dependent enzyme -
  NI376_RS03290 (NI376_03285) proC 704922..705743 (+) 822 WP_308374158.1 pyrroline-5-carboxylate reductase -
  NI376_RS03295 (NI376_03290) - 705753..706286 (+) 534 WP_010379021.1 YggT family protein -
  NI376_RS03300 (NI376_03295) - 706297..706731 (+) 435 WP_308374160.1 DUF4426 domain-containing protein -
  NI376_RS03305 (NI376_03300) - 706740..707333 (+) 594 WP_308374161.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41134.98 Da        Isoelectric Point: 6.0556

>NTDB_id=702756 NI376_RS03275 WP_010607713.1 702012..703133(-) (pilU) [Pseudoalteromonas piscicida strain BM7A]
MNIEPFLQTMANQQASDLFVSAGLAVSAKIDGELRALSEESLDAEQSLNLVTSIMSDKQKQEFFNTKECNFAIANDIGRF
RVSAFWQRDCAGMVIRRIVTAIPDVNELGLPSVLTDVIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIEDP
IEFVHQHQKSIITQREVGIDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDRIM
HLVPKEKHDKLKYDLALNLRAIVAQQLVPSSKGEGREAAIEVLLNSPLVAELIKKGDIGSIKEAMAKSKDMGMQTFDQAL
FELYKQQRINYADALHHADSPNDLRLMIKLQNNEQKGAGFLQGVTVDGLDSKN

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=702756 NI376_RS03275 WP_010607713.1 702012..703133(-) (pilU) [Pseudoalteromonas piscicida strain BM7A]
ATGAATATAGAACCATTTTTGCAAACGATGGCAAATCAGCAAGCATCCGACTTATTCGTCTCGGCGGGGCTTGCGGTGAG
CGCCAAAATCGATGGCGAACTTAGAGCGTTGAGTGAAGAAAGCTTAGATGCCGAGCAGTCACTGAATCTTGTTACCTCGA
TTATGAGCGACAAGCAAAAGCAAGAATTCTTTAACACTAAAGAGTGTAACTTTGCGATTGCCAATGATATCGGGCGTTTT
CGTGTTTCAGCATTTTGGCAACGAGACTGTGCGGGCATGGTTATCCGCCGTATCGTTACTGCTATTCCCGATGTCAATGA
GCTAGGTTTGCCGTCGGTACTGACCGATGTGATCATGTCCAAACGTGGCTTGGTGTTATTTGTAGGTGGTACGGGCACGG
GTAAGTCAACCTCGCTTGCTGCACTGCTCGGTTATCGTAATCGTAACCAGCGTGGCCATATTTTGACCATTGAAGATCCT
ATTGAGTTTGTGCATCAACACCAAAAAAGCATCATTACTCAGCGTGAAGTGGGCATTGATACTGACAGTTTCGAATCTGC
CTTAAAAAGTTCATTACGCCAAGCGCCAGATGTTATTTTAATTGGTGAAATTCGCTCGCAAGAAACCATGGAGTATGCAC
TGAGCTTTGCTGAAACGGGGCATTTATGCGTTGCAACCTTACACGCTAATAATGCTAACCAAGCCATCGACCGTATCATG
CACTTAGTCCCTAAAGAAAAGCACGATAAACTGAAGTACGATTTGGCGCTGAACTTGCGCGCTATTGTGGCGCAACAACT
TGTGCCTTCTTCAAAAGGTGAAGGTCGAGAAGCAGCCATTGAAGTACTACTGAATTCCCCACTCGTTGCCGAGCTTATTA
AAAAAGGCGATATTGGTTCGATTAAAGAAGCAATGGCAAAATCGAAAGACATGGGCATGCAAACCTTTGACCAAGCGTTG
TTTGAGCTTTATAAGCAGCAGCGGATCAACTATGCCGACGCACTACATCATGCTGACTCGCCAAATGATCTGCGCCTGAT
GATCAAGCTGCAAAATAATGAACAAAAAGGTGCGGGCTTCCTACAAGGGGTTACGGTAGATGGGTTGGATTCGAAGAACT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9JUM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

56.764

100

0.574
  pilU Pseudomonas stutzeri DSM 10701

60.399

94.102

0.568
  pilU Vibrio cholerae strain A1552

56.131

98.391

0.552
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.5

96.515

0.41
  pilT Acinetobacter baylyi ADP1

40.936

91.689

0.375
  pilT Legionella pneumophila strain ERS1305867

41.742

89.276

0.373
  pilT Legionella pneumophila strain Lp02

41.742

89.276

0.373
  pilT Pseudomonas aeruginosa PAK

40.29

92.493

0.373
  pilT Acinetobacter nosocomialis M2

40.351

91.689

0.37
  pilT Acinetobacter baumannii D1279779

40.351

91.689

0.37
  pilT Acinetobacter baumannii strain A118

40.351

91.689

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.42

92.493

0.365