Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NI389_RS06585 Genome accession   NZ_CP099917
Coordinates   1428529..1429575 (-) Length   348 a.a.
NCBI ID   WP_208844059.1    Uniprot ID   -
Organism   Pseudoalteromonas xiamenensis strain PSDB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1423529..1434575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI389_RS06555 (NI389_06540) rsmE 1423582..1424316 (+) 735 WP_308362102.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NI389_RS06560 (NI389_06545) gshB 1424372..1425328 (+) 957 WP_308362103.1 glutathione synthase -
  NI389_RS06565 (NI389_06550) - 1425349..1425906 (+) 558 WP_308362104.1 YqgE/AlgH family protein -
  NI389_RS06570 (NI389_06555) ruvX 1426063..1426515 (+) 453 WP_208844057.1 Holliday junction resolvase RuvX -
  NI389_RS06575 (NI389_06560) - 1426516..1427412 (-) 897 WP_244369878.1 hypothetical protein -
  NI389_RS06580 (NI389_06565) pilU 1427393..1428517 (-) 1125 WP_208844058.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NI389_RS06585 (NI389_06570) pilT 1428529..1429575 (-) 1047 WP_208844059.1 type IV pilus twitching motility protein PilT Machinery gene
  NI389_RS06590 (NI389_06575) - 1429605..1430288 (+) 684 WP_308362105.1 YggS family pyridoxal phosphate-dependent enzyme -
  NI389_RS06595 (NI389_06580) proC 1430303..1431124 (+) 822 WP_308362106.1 pyrroline-5-carboxylate reductase -
  NI389_RS06600 (NI389_06585) - 1431134..1431667 (+) 534 WP_308362107.1 YggT family protein -
  NI389_RS06605 (NI389_06590) - 1431780..1432194 (+) 415 Protein_1286 DUF4426 domain-containing protein -
  NI389_RS06610 (NI389_06595) - 1432197..1432790 (+) 594 WP_308362108.1 XTP/dITP diphosphatase -
  NI389_RS06615 (NI389_06600) hemW 1432787..1433923 (+) 1137 WP_308362109.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38674.06 Da        Isoelectric Point: 6.4423

>NTDB_id=702521 NI389_RS06585 WP_208844059.1 1428529..1429575(-) (pilT) [Pseudoalteromonas xiamenensis strain PSDB]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPVLEAKDVNSLVYDIMNDNQRRDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLDDLGAPDIFRKISENPRGLVLVTGPTGSGKSTTLAAMVDYINQNKHHHILTIED
PIEFVHQNKLSLINQREVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTVDRI
IDVFPGEEKDMVRSMLSESLQAVISQTLVKKSGGGRVAAHEIMLGIPAIRNLIREDKIAQMYSAIQTGASHGMQTMDQCL
MNLVNRGIISQQEARTKAHDKNQFGYGM

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=702521 NI389_RS06585 WP_208844059.1 1428529..1429575(-) (pilT) [Pseudoalteromonas xiamenensis strain PSDB]
ATGGATATTACCGAATTATTAGCCTTTAGTGTGCAACACAAAGCGTCTGATTTACACCTTTCTTCAGGTGTGTCGCCGAT
GATCCGAGTTGATGGCGACGTCAGGCGCATTAATATCCCTGTGCTTGAAGCTAAAGACGTCAATAGTCTCGTGTATGACA
TCATGAATGATAACCAGCGCAGAGACTATGAGCAAAATCTTGAGGTGGATTTTTCATTTGAAGTACCTAACCTTGCTCGT
TTTCGTGTGAATGCGTTCAATTCAAACCGTGGTCCTGCTGCGGTATTTCGTACGATTCCAAGCGAAGTGTTGACGTTGGA
TGATTTAGGTGCGCCCGATATTTTCAGAAAAATCTCTGAAAATCCAAGAGGTTTGGTGCTTGTAACCGGACCGACAGGTT
CCGGTAAATCAACAACGCTGGCCGCAATGGTGGACTATATAAACCAAAATAAACACCACCATATTTTAACCATCGAAGAT
CCAATTGAATTTGTTCACCAAAACAAGCTCAGTCTTATTAACCAGCGTGAAGTGCATCGCGACACACATTCGTTTTCGGC
GGCTCTGCGCAGCGCACTACGTGAAGATCCTGATGTCATTCTGGTGGGTGAATTACGGGATCTGGAAACCATACGCCTTG
CGATGACCGCAGCGGAAACTGGCCACTTGGTGTTTGGTACGCTTCATACAACGTCTGCACCAAAAACGGTTGACCGTATT
ATCGACGTATTCCCAGGTGAAGAAAAAGACATGGTTCGTTCTATGTTATCCGAATCATTGCAAGCCGTTATCTCACAAAC
CTTGGTGAAAAAATCCGGCGGTGGTCGTGTTGCTGCGCATGAAATCATGTTAGGTATTCCTGCCATCCGAAACTTAATCC
GTGAAGATAAAATCGCACAAATGTATTCGGCTATCCAAACCGGTGCTTCGCATGGCATGCAGACGATGGATCAGTGCCTC
ATGAATTTAGTGAATCGTGGCATTATTTCACAGCAAGAAGCTCGAACTAAAGCGCACGATAAAAATCAGTTTGGTTACGG
GATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.779

98.851

0.779
  pilT Acinetobacter baumannii strain A118

78.779

98.851

0.779
  pilT Acinetobacter nosocomialis M2

78.779

98.851

0.779
  pilT Acinetobacter baylyi ADP1

76.163

98.851

0.753
  pilT Pseudomonas stutzeri DSM 10701

75.581

98.851

0.747
  pilT Pseudomonas aeruginosa PAK

75.581

98.851

0.747
  pilT Legionella pneumophila strain Lp02

72.384

98.851

0.716
  pilT Legionella pneumophila strain ERS1305867

72.384

98.851

0.716
  pilT Neisseria meningitidis 8013

69.452

99.713

0.693
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

96.839

0.69
  pilT Vibrio cholerae strain A1552

71.217

96.839

0.69
  pilT Neisseria gonorrhoeae MS11

69.164

99.713

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.138

0.517
  pilU Vibrio cholerae strain A1552

41.399

98.563

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.942

98.563

0.394
  pilU Acinetobacter baylyi ADP1

38.372

98.851

0.379