Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NI389_RS06580 Genome accession   NZ_CP099917
Coordinates   1427393..1428517 (-) Length   374 a.a.
NCBI ID   WP_208844058.1    Uniprot ID   A0A975DIV1
Organism   Pseudoalteromonas xiamenensis strain PSDB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1422393..1433517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI389_RS06550 (NI389_06535) - 1423002..1423511 (+) 510 WP_244369876.1 hypothetical protein -
  NI389_RS06555 (NI389_06540) rsmE 1423582..1424316 (+) 735 WP_308362102.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NI389_RS06560 (NI389_06545) gshB 1424372..1425328 (+) 957 WP_308362103.1 glutathione synthase -
  NI389_RS06565 (NI389_06550) - 1425349..1425906 (+) 558 WP_308362104.1 YqgE/AlgH family protein -
  NI389_RS06570 (NI389_06555) ruvX 1426063..1426515 (+) 453 WP_208844057.1 Holliday junction resolvase RuvX -
  NI389_RS06575 (NI389_06560) - 1426516..1427412 (-) 897 WP_244369878.1 hypothetical protein -
  NI389_RS06580 (NI389_06565) pilU 1427393..1428517 (-) 1125 WP_208844058.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NI389_RS06585 (NI389_06570) pilT 1428529..1429575 (-) 1047 WP_208844059.1 type IV pilus twitching motility protein PilT Machinery gene
  NI389_RS06590 (NI389_06575) - 1429605..1430288 (+) 684 WP_308362105.1 YggS family pyridoxal phosphate-dependent enzyme -
  NI389_RS06595 (NI389_06580) proC 1430303..1431124 (+) 822 WP_308362106.1 pyrroline-5-carboxylate reductase -
  NI389_RS06600 (NI389_06585) - 1431134..1431667 (+) 534 WP_308362107.1 YggT family protein -
  NI389_RS06605 (NI389_06590) - 1431780..1432194 (+) 415 Protein_1286 DUF4426 domain-containing protein -
  NI389_RS06610 (NI389_06595) - 1432197..1432790 (+) 594 WP_308362108.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41428.54 Da        Isoelectric Point: 5.9705

>NTDB_id=702520 NI389_RS06580 WP_208844058.1 1427393..1428517(-) (pilU) [Pseudoalteromonas xiamenensis strain PSDB]
MSSPLEPYLHMMVERKASDMFVSAGLPISAKIDGDLQPLDDVEISPERALQFVESVMSEAQKSEFHTTKECNFAIANDEG
RFRISAFWQRDCAGMVIRRIVTQIPDVADLKLPSVLTDVIMMKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHTHKKSMITQREVGIDTDSFEAALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLIPTSDGEGRVAAIEVMLNSPLVAELIKKGDVGSIKEVMAKSKEQGMQTFDQ
ALFELYRTQQINYADALHHADSPNDLRLMIKLQNNEQKGAGFLQGVTVDGLGKD

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=702520 NI389_RS06580 WP_208844058.1 1427393..1428517(-) (pilU) [Pseudoalteromonas xiamenensis strain PSDB]
ATGTCATCACCACTAGAACCTTATTTACATATGATGGTTGAGCGTAAAGCATCGGACATGTTTGTTTCTGCGGGACTACC
TATCAGTGCTAAAATTGATGGCGATTTGCAGCCTCTAGACGACGTTGAAATCAGTCCCGAGCGGGCGCTTCAGTTTGTTG
AATCGGTAATGAGTGAAGCGCAAAAAAGTGAGTTTCATACGACTAAAGAATGTAACTTTGCAATTGCCAACGATGAAGGG
CGTTTTCGTATTTCAGCGTTTTGGCAACGAGATTGTGCTGGGATGGTTATTCGTCGAATCGTGACACAGATTCCAGATGT
CGCTGACTTAAAACTGCCTTCGGTACTTACCGACGTGATCATGATGAAACGTGGTCTAGTGTTGTTCGTGGGTGGTACAG
GAACGGGTAAATCTACGTCACTTGCCGCGCTCATCGGTTATCGAAATCGTAATCAACGTGGGCACATTCTGACAATCGAA
GACCCCATTGAGTTTGTGCATACGCACAAGAAAAGTATGATTACGCAACGGGAAGTTGGTATTGATACCGACAGTTTTGA
AGCCGCGTTGAAAAGCTCATTGCGTCAAGCGCCAGATGTTATTCTCATTGGTGAAATTCGTTCTCAAGAAACAATGGAAT
ACGCGTTAAGTTTTGCGGAAACCGGGCATTTATGCGTTGCAACATTGCACGCGAACAATGCGAACCAAGCGATTGACCGT
ATTATGCACTTAGTGCCAAAAGAGAAACATGACAAGCTTAAATACGATTTAGCGTTGAACTTGCGCGCGATTGTCGCTCA
GCAGTTGATCCCAACGTCGGATGGCGAAGGCCGTGTTGCAGCCATTGAAGTCATGTTGAATTCGCCATTAGTCGCCGAGT
TGATTAAGAAAGGCGATGTTGGCTCTATCAAAGAAGTGATGGCTAAATCCAAAGAGCAAGGCATGCAAACGTTTGACCAA
GCTTTGTTCGAACTATATCGAACACAACAAATCAACTACGCGGATGCGCTTCATCATGCAGACTCGCCAAACGACTTACG
TTTGATGATTAAACTTCAAAATAATGAGCAAAAGGGAGCTGGCTTCTTGCAAGGTGTCACCGTAGATGGTTTGGGTAAAG
ATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.605

93.316

0.575
  pilU Acinetobacter baylyi ADP1

56.065

99.198

0.556
  pilU Vibrio cholerae strain A1552

55.616

97.594

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.539

95.187

0.414