Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NI390_RS01955 Genome accession   NZ_CP099915
Coordinates   399937..400974 (+) Length   345 a.a.
NCBI ID   WP_024374563.1    Uniprot ID   -
Organism   Vibrio fluvialis strain Isc7A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 394937..405974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI390_RS01920 (NI390_01920) hemW 395164..396336 (-) 1173 WP_308413453.1 radical SAM family heme chaperone HemW -
  NI390_RS01925 (NI390_01925) - 396336..396935 (-) 600 WP_222783423.1 XTP/dITP diphosphatase -
  NI390_RS01930 (NI390_01930) - 396959..397390 (-) 432 WP_024374558.1 DUF4426 domain-containing protein -
  NI390_RS01935 (NI390_01935) yggU 397450..397740 (-) 291 WP_158129775.1 DUF167 family protein YggU -
  NI390_RS01940 (NI390_01940) - 397740..398297 (-) 558 WP_024374560.1 YggT family protein -
  NI390_RS01945 (NI390_01945) proC 398349..399167 (-) 819 WP_158142400.1 pyrroline-5-carboxylate reductase -
  NI390_RS01950 (NI390_01950) - 399204..399911 (-) 708 WP_154184781.1 YggS family pyridoxal phosphate-dependent enzyme -
  NI390_RS01955 (NI390_01955) pilT 399937..400974 (+) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  NI390_RS01960 (NI390_01960) pilU 400986..402092 (+) 1107 WP_308413361.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NI390_RS01965 (NI390_01965) ruvX 402157..402582 (-) 426 WP_024374565.1 Holliday junction resolvase RuvX -
  NI390_RS01970 (NI390_01970) - 402644..403207 (-) 564 WP_032080468.1 YqgE/AlgH family protein -
  NI390_RS01975 (NI390_01975) gshB 403247..404203 (-) 957 WP_024374566.1 glutathione synthase -
  NI390_RS01980 (NI390_01980) rsmE 404216..404947 (-) 732 WP_032080467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NI390_RS01985 (NI390_01985) - 405044..405745 (-) 702 WP_032080466.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38227.83 Da        Isoelectric Point: 6.5038

>NTDB_id=702471 NI390_RS01955 WP_024374563.1 399937..400974(+) (pilT) [Vibrio fluvialis strain Isc7A]
MDISELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLIFEIMNDAQRSEYEEKLEVDFSFALPDIGR
FRVNAFHQSRGASAVFRTIPTVIPTLDQLEAPEIFHKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINATHNKHVLTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRLGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGASHGMQTMEQHA
KQLIAQGLVDPAEVQKKIELEMSAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=702471 NI390_RS01955 WP_024374563.1 399937..400974(+) (pilT) [Vibrio fluvialis strain Isc7A]
ATGGATATCTCTGAGTTACTGGAATTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCGGGTGTTCCTCCTAT
GGTACGCATCGATGGTGAAGTGAGAAAGCTTGGCGTACCTGCGTTTACACACGCTGATGTGCATCGATTAATTTTTGAAA
TTATGAATGATGCCCAGCGCAGTGAGTACGAAGAAAAGCTGGAAGTGGACTTTTCCTTTGCGTTACCGGACATCGGCCGG
TTTCGTGTCAACGCTTTCCATCAGTCGCGCGGCGCTTCTGCGGTATTTCGTACCATTCCGACCGTGATCCCGACTCTGGA
CCAGCTTGAGGCTCCGGAAATTTTCCACAAAATCGCCAATTACGAAAAAGGTTTGGTGCTGGTGACCGGACCGACTGGCT
CGGGTAAATCAACCACGTTAGCGGCGATGGTCGATTACATCAACGCAACCCATAACAAGCATGTGCTGACTATCGAAGAT
CCGATTGAGTTTGTGCACAGTAACAACAAATGCTTGATCAACCAACGTGAAGTACACCGCGATACCCACAGTTTTAAAAA
CGCGCTGCGCAGCGCATTGCGTGAAGATCCGGATGTGATTCTGGTCGGTGAGTTGCGCGATCAGGAAACCATCAGCTTAG
CGCTGACGGCGGCGGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCAGTTCGGCGGCGAAAACCATTGACCGCATC
ATCGATGTGTTCCCGGGCAGCGACAAGGACATGGTACGTTCAATGTTGTCAGAATCGCTGCGTGCGGTGATTGCGCAAAA
ACTGCTCAAACGTCTGGGTGGCGGGCGTGTGGCGTGTCATGAAATTATGTTGGCGACGCCTGCTATCCGTAACCTGATTC
GCGAAGATAAAGTCGCGCAGATGTATTCGATCATTCAGACTGGCGCCTCCCATGGAATGCAGACTATGGAGCAGCATGCC
AAACAGTTGATCGCGCAAGGGCTGGTGGATCCGGCGGAAGTGCAGAAGAAAATTGAACTGGAAATGTCTGCGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.754

100

0.928
  pilT Vibrio cholerae strain A1552

92.754

100

0.928
  pilT Acinetobacter baumannii D1279779

71.601

95.942

0.687
  pilT Acinetobacter baumannii strain A118

71.601

95.942

0.687
  pilT Acinetobacter nosocomialis M2

71.299

95.942

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.059

98.551

0.661
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

62.059

98.551

0.612
  pilT Neisseria gonorrhoeae MS11

61.765

98.551

0.609
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.424

95.652

0.501
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.867

96.232

0.403
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374