Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NI390_RS01960 Genome accession   NZ_CP099915
Coordinates   400986..402092 (+) Length   368 a.a.
NCBI ID   WP_308413361.1    Uniprot ID   -
Organism   Vibrio fluvialis strain Isc7A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 395986..407092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI390_RS01925 (NI390_01925) - 396336..396935 (-) 600 WP_222783423.1 XTP/dITP diphosphatase -
  NI390_RS01930 (NI390_01930) - 396959..397390 (-) 432 WP_024374558.1 DUF4426 domain-containing protein -
  NI390_RS01935 (NI390_01935) yggU 397450..397740 (-) 291 WP_158129775.1 DUF167 family protein YggU -
  NI390_RS01940 (NI390_01940) - 397740..398297 (-) 558 WP_024374560.1 YggT family protein -
  NI390_RS01945 (NI390_01945) proC 398349..399167 (-) 819 WP_158142400.1 pyrroline-5-carboxylate reductase -
  NI390_RS01950 (NI390_01950) - 399204..399911 (-) 708 WP_154184781.1 YggS family pyridoxal phosphate-dependent enzyme -
  NI390_RS01955 (NI390_01955) pilT 399937..400974 (+) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  NI390_RS01960 (NI390_01960) pilU 400986..402092 (+) 1107 WP_308413361.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NI390_RS01965 (NI390_01965) ruvX 402157..402582 (-) 426 WP_024374565.1 Holliday junction resolvase RuvX -
  NI390_RS01970 (NI390_01970) - 402644..403207 (-) 564 WP_032080468.1 YqgE/AlgH family protein -
  NI390_RS01975 (NI390_01975) gshB 403247..404203 (-) 957 WP_024374566.1 glutathione synthase -
  NI390_RS01980 (NI390_01980) rsmE 404216..404947 (-) 732 WP_032080467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NI390_RS01985 (NI390_01985) - 405044..405745 (-) 702 WP_032080466.1 endonuclease -
  NI390_RS01990 (NI390_01990) - 405864..406364 (-) 501 WP_172557955.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41216.17 Da        Isoelectric Point: 6.0546

>NTDB_id=702472 NI390_RS01960 WP_308413361.1 400986..402092(+) (pilU) [Vibrio fluvialis strain Isc7A]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAQDEKLDEAQVSALLAAMMDEARHADYLATREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPAVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKELREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGDLHELKSTMAKSKEVGMQTFDQALY
QLVVENKISEQDALHCADSANDLRLMLKTQRGDVSSSGSLDHVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=702472 NI390_RS01960 WP_308413361.1 400986..402092(+) (pilU) [Vibrio fluvialis strain Isc7A]
ATGCAACTCGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCAGATCTATATATTACTGTGGGCGCCCCCGTGCT
GTACCGGGTCGATGGTGAACTGCGCGCGCAGGATGAGAAGCTGGATGAAGCGCAGGTCAGCGCGCTGCTGGCTGCGATGA
TGGATGAAGCGCGCCATGCGGACTATCTGGCGACGCGTGAAGCTAACTTTGCCATCGTGCGTGATTTGGGGCGTTTTCGC
GTCAGCGCTTTTTTTCAGCGCGAGCTACCTGGTGCGGTGATTCGCCGCATCGAAACGCAGATTCCGACCTTTGATGAACT
CAAACTGCCAGCAGTGCTGAAAGATCTCTCGATTGCCAAACGCGGTTTAGTGTTGGTGGTGGGGGCGACAGGATCGGGCA
AATCGACCACCATGGCCGCGATGACTGGCTATCGCAACACGCACCGCAGCGGGCATATTCTCACCGTCGAAGATCCGATT
GAATTTGTCCATGAGCACCAGCGCTGCATCGTGACACAGCGTGAAGTGGGATTGGACACCGAAAGCTATGAAGTGGCGCT
GAAGAACTCGCTACGCCAGGCGCCGGATATGATTTTGATCGGAGAGATCCGCAGCCGTGAAACCATGGAATACGCAATGA
CCTTCGCCGAAACCGGTCACTTGTGCATGGCAACTCTGCACGCCAACAACGCCAACCAAGCGCTCGAACGTATTTTGCAT
CTGGTGCCAAAGGAGCTGCGTGAACAATTTCTGTTTGACCTGTCGATGAACCTCAAAGGCGTCGTAGGTCAGCAATTGAT
CCGCGATAAAAACGGGCAAGGGCGTCACGGCGTGTTTGAAGTGTTGCTCAACAGTCCACGGGTGTCGGACATCATCCGAC
GCGGCGATTTGCATGAGCTGAAAAGCACCATGGCGAAATCGAAAGAGGTTGGCATGCAGACTTTTGATCAGGCGTTGTAT
CAGTTGGTGGTCGAGAACAAGATCAGCGAACAGGATGCGCTGCACTGTGCCGATTCGGCCAACGATCTGCGCTTGATGCT
CAAGACTCAGCGGGGTGATGTGAGCAGCAGCGGTTCACTGGATCATGTGCAAATCGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.054

100

0.851
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.385

88.315

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Pseudomonas aeruginosa PAK

41.194

91.033

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.701

91.033

0.361