Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NH461_RS02355 Genome accession   NZ_CP099734
Coordinates   477310..478341 (+) Length   343 a.a.
NCBI ID   WP_261601729.1    Uniprot ID   -
Organism   Photobacterium sp. TY1-4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472310..483341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH461_RS02320 (NH461_02315) hemW 472336..473493 (-) 1158 WP_261601722.1 radical SAM family heme chaperone HemW -
  NH461_RS02325 (NH461_02320) - 473493..474086 (-) 594 WP_261601723.1 XTP/dITP diphosphatase -
  NH461_RS02330 (NH461_02325) - 474219..474644 (-) 426 WP_261601724.1 DUF4426 domain-containing protein -
  NH461_RS02335 (NH461_02330) yggU 474866..475162 (-) 297 WP_261601725.1 DUF167 family protein YggU -
  NH461_RS02340 (NH461_02335) - 475159..475716 (-) 558 WP_261601726.1 YggT family protein -
  NH461_RS02345 (NH461_02340) proC 475726..476544 (-) 819 WP_261601727.1 pyrroline-5-carboxylate reductase -
  NH461_RS02350 (NH461_02345) - 476573..477286 (-) 714 WP_261601728.1 YggS family pyridoxal phosphate-dependent enzyme -
  NH461_RS02355 (NH461_02350) pilT 477310..478341 (+) 1032 WP_261601729.1 type IV pilus twitching motility protein PilT Machinery gene
  NH461_RS02360 (NH461_02355) pilU 478367..479473 (+) 1107 WP_261601730.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NH461_RS02365 (NH461_02360) ruvX 479838..480266 (-) 429 WP_261601731.1 Holliday junction resolvase RuvX -
  NH461_RS02370 (NH461_02365) - 480263..480832 (-) 570 WP_261601732.1 YqgE/AlgH family protein -
  NH461_RS02375 (NH461_02370) gshB 480981..481928 (-) 948 WP_261601733.1 glutathione synthase -
  NH461_RS02380 (NH461_02375) rsmE 481960..482691 (-) 732 WP_261601734.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 38010.54 Da        Isoelectric Point: 6.3858

>NTDB_id=701389 NH461_RS02355 WP_261601729.1 477310..478341(+) (pilT) [Photobacterium sp. TY1-4]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPELEHGEVHRLVFDIMTDAQRREFEEKLEVDFSFELPQVGR
FRVNAFHQSRGCAAVFRTIPVQVPSLSSLNVPDIFYRIAQCQRGLVLVTGATGSGKSTTIAALVDYINENFNRHILTIED
PVEFVHHGKRCLVNQREVHRDTHSFHQALRSALREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTIDRI
IDVFPGNDKAMVRSMLSESLRAVVSQNLLKCVTGGRVAAHEVMMATPAIRNLIREDKVAQMYSMIQTGSALGMQTMEQSV
KMLVAQGIVDAEEGRRIVDSGQL

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=701389 NH461_RS02355 WP_261601729.1 477310..478341(+) (pilT) [Photobacterium sp. TY1-4]
ATGGATATCACCGAACTACTGGACTTTAGTGTAAAGCATAATGCCTCCGATCTGCACCTTTCTGCCGGAGTGCCGCCGAT
GATTCGGGTGGATGGCGATGTCAGAAAGCTCAGCCTGCCGGAACTGGAGCATGGGGAAGTGCACCGGTTGGTGTTTGATA
TTATGACCGACGCCCAACGCCGGGAGTTTGAAGAGAAGCTGGAAGTGGACTTCTCGTTCGAGCTGCCGCAGGTCGGCCGC
TTCCGGGTCAACGCCTTTCACCAGTCGCGCGGCTGCGCGGCGGTGTTCCGGACCATTCCGGTCCAGGTGCCGTCCTTGAG
CTCACTCAATGTCCCTGACATCTTTTATCGCATTGCCCAGTGCCAGCGCGGCCTGGTTCTGGTGACCGGTGCGACGGGCT
CGGGGAAATCGACCACGATCGCAGCGCTGGTCGATTACATCAATGAAAATTTTAACCGTCATATTCTGACCATTGAAGAT
CCGGTGGAATTTGTCCATCACGGCAAACGTTGCCTGGTGAACCAGCGCGAGGTTCATCGCGATACCCACAGCTTCCACCA
GGCATTGCGCTCGGCGCTGCGGGAAGATCCGGATGTGATTGTGGTCGGTGAGCTGCGCGATCAGGAAACCATCAGCCTGG
CGCTGACGGCGGCTGAAACCGGCCACCTGGTGCTGGGGACGCTGCATACCAGCTCGGCGGCGAAAACCATTGACCGGATC
ATTGATGTGTTCCCGGGCAATGATAAGGCGATGGTGCGCTCGATGCTGTCGGAGTCGCTGCGGGCGGTGGTATCGCAGAA
CCTGCTCAAGTGTGTCACCGGCGGGCGGGTGGCGGCCCATGAAGTGATGATGGCAACCCCGGCGATCCGCAACCTGATCC
GGGAAGATAAGGTGGCGCAGATGTACTCGATGATCCAGACCGGCTCTGCGCTGGGGATGCAGACCATGGAGCAGAGCGTC
AAGATGCTGGTGGCCCAGGGGATTGTCGATGCCGAGGAAGGGCGCCGGATTGTCGACAGCGGCCAGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

77.778

97.085

0.755
  pilT Vibrio cholerae strain A1552

77.778

97.085

0.755
  pilT Acinetobacter baumannii D1279779

70.448

97.668

0.688
  pilT Acinetobacter baumannii strain A118

70.448

97.668

0.688
  pilT Acinetobacter nosocomialis M2

70.149

97.668

0.685
  pilT Pseudomonas aeruginosa PAK

69.552

97.668

0.679
  pilT Acinetobacter baylyi ADP1

68.235

99.125

0.676
  pilT Pseudomonas stutzeri DSM 10701

68.358

97.668

0.668
  pilT Legionella pneumophila strain Lp02

68.693

95.918

0.659
  pilT Legionella pneumophila strain ERS1305867

68.693

95.918

0.659
  pilT Neisseria meningitidis 8013

64.688

98.251

0.636
  pilT Neisseria gonorrhoeae MS11

64.392

98.251

0.633
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.387

99.417

0.481
  pilU Pseudomonas stutzeri DSM 10701

41.056

99.417

0.408
  pilU Vibrio cholerae strain A1552

39.766

99.708

0.397
  pilU Acinetobacter baylyi ADP1

39.752

93.878

0.373