Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NH461_RS02360 Genome accession   NZ_CP099734
Coordinates   478367..479473 (+) Length   368 a.a.
NCBI ID   WP_261601730.1    Uniprot ID   -
Organism   Photobacterium sp. TY1-4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 473367..484473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH461_RS02325 (NH461_02320) - 473493..474086 (-) 594 WP_261601723.1 XTP/dITP diphosphatase -
  NH461_RS02330 (NH461_02325) - 474219..474644 (-) 426 WP_261601724.1 DUF4426 domain-containing protein -
  NH461_RS02335 (NH461_02330) yggU 474866..475162 (-) 297 WP_261601725.1 DUF167 family protein YggU -
  NH461_RS02340 (NH461_02335) - 475159..475716 (-) 558 WP_261601726.1 YggT family protein -
  NH461_RS02345 (NH461_02340) proC 475726..476544 (-) 819 WP_261601727.1 pyrroline-5-carboxylate reductase -
  NH461_RS02350 (NH461_02345) - 476573..477286 (-) 714 WP_261601728.1 YggS family pyridoxal phosphate-dependent enzyme -
  NH461_RS02355 (NH461_02350) pilT 477310..478341 (+) 1032 WP_261601729.1 type IV pilus twitching motility protein PilT Machinery gene
  NH461_RS02360 (NH461_02355) pilU 478367..479473 (+) 1107 WP_261601730.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NH461_RS02365 (NH461_02360) ruvX 479838..480266 (-) 429 WP_261601731.1 Holliday junction resolvase RuvX -
  NH461_RS02370 (NH461_02365) - 480263..480832 (-) 570 WP_261601732.1 YqgE/AlgH family protein -
  NH461_RS02375 (NH461_02370) gshB 480981..481928 (-) 948 WP_261601733.1 glutathione synthase -
  NH461_RS02380 (NH461_02375) rsmE 481960..482691 (-) 732 WP_261601734.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NH461_RS02385 (NH461_02380) endA 482845..483549 (-) 705 WP_261601735.1 deoxyribonuclease I -
  NH461_RS02390 (NH461_02385) - 483673..484164 (-) 492 WP_261601736.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41370.60 Da        Isoelectric Point: 6.3188

>NTDB_id=701390 NH461_RS02360 WP_261601730.1 478367..479473(+) (pilU) [Photobacterium sp. TY1-4]
MTLQQILEEMVRCKASDLYITVDSPGLLRVDGHLHPVGDVLDRRAVDGLFDDAMDDQQQQEFTATKEANFALVRGELRFR
ISAFWQREMPGMVIRRIETEIPDLHALNLPMDMQKMALAKRGLVLVVGATGSGKSTTMAALTGYRNNNRSGHILTVEDPI
EFVHKHKRCIVTQREVGLDTESYDVALKNSLRQAPDMILIGEIRTPETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERREQFLFDLSLNLKCILAQQLIPDAKGIGRHGAFELLLNTPRIADLIRRGELHEIKEAMAKSTESGMMTFDQSLF
ELFSQGKITEQDALHHADSANDLRLMIKVGSKTQQAKNALDGVTIDMR

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=701390 NH461_RS02360 WP_261601730.1 478367..479473(+) (pilU) [Photobacterium sp. TY1-4]
ATGACATTACAGCAGATCCTTGAAGAGATGGTGCGCTGCAAAGCCTCGGATCTCTATATTACGGTGGACTCGCCGGGCCT
GCTTCGGGTTGACGGTCATTTGCATCCGGTCGGCGACGTGCTCGATCGCCGGGCGGTGGATGGGTTATTTGATGATGCGA
TGGACGATCAGCAGCAGCAGGAGTTTACGGCCACCAAGGAGGCGAACTTTGCCCTGGTGCGGGGAGAGCTGCGCTTTCGG
ATCAGTGCCTTCTGGCAGCGGGAAATGCCGGGGATGGTGATCCGCCGGATTGAAACGGAGATCCCGGATCTGCATGCGCT
CAATCTGCCGATGGACATGCAAAAGATGGCCCTGGCCAAGCGAGGGCTGGTGCTGGTGGTCGGGGCCACCGGCTCGGGCA
AATCGACCACCATGGCAGCGCTGACCGGCTATCGCAACAACAACCGCAGCGGTCATATTCTGACGGTGGAAGATCCGATT
GAGTTCGTGCATAAGCACAAGCGTTGCATCGTCACCCAGCGCGAAGTCGGGCTGGATACCGAAAGCTACGATGTCGCGTT
GAAAAATTCCTTGCGCCAGGCGCCGGATATGATCCTGATCGGTGAGATCCGGACACCGGAGACCATGGAGTACGCGATGA
ACTTCGCCGAAACCGGCCACCTGTGCATGGCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGAGCGGATCCTACAC
TTAGTGCCGAAAGAGCGACGGGAGCAGTTCCTGTTTGATTTGTCCCTGAACCTGAAATGTATTCTGGCCCAGCAGTTGAT
CCCGGATGCCAAGGGGATCGGGCGTCACGGTGCGTTCGAGTTGTTGCTCAATACGCCGCGGATCGCCGATTTGATCCGTC
GCGGCGAGCTGCACGAGATAAAGGAAGCGATGGCGAAATCGACCGAGTCGGGCATGATGACCTTTGATCAGTCGCTGTTC
GAGCTGTTTAGCCAGGGCAAAATTACCGAGCAGGACGCGCTGCACCATGCCGATTCGGCCAATGATTTACGTCTGATGAT
CAAAGTCGGCAGCAAGACCCAGCAGGCCAAAAACGCGCTTGATGGCGTGACCATTGATATGCGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

70.845

99.728

0.707
  pilU Pseudomonas stutzeri DSM 10701

56.8

100

0.579
  pilU Acinetobacter baylyi ADP1

52.877

99.185

0.524
  pilT Legionella pneumophila strain Lp02

41.791

91.033

0.38
  pilT Legionella pneumophila strain ERS1305867

41.791

91.033

0.38
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.154

88.315

0.372