Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NHF51_RS02705 Genome accession   NZ_CP099717
Coordinates   548409..549443 (-) Length   344 a.a.
NCBI ID   WP_252995562.1    Uniprot ID   A0AAE9SCS3
Organism   Aeromonas encheleia strain SOD01     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 543409..554443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHF51_RS02685 (NHF51_02685) - 544225..544935 (-) 711 WP_252995560.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NHF51_RS02690 (NHF51_02690) srmB 545064..546284 (+) 1221 WP_042652084.1 ATP-dependent RNA helicase SrmB -
  NHF51_RS02695 (NHF51_02695) yaaA 546469..547242 (-) 774 WP_252995561.1 peroxide stress protein YaaA -
  NHF51_RS02700 (NHF51_02700) pilU 547274..548383 (-) 1110 WP_042652086.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NHF51_RS02705 (NHF51_02705) pilT 548409..549443 (-) 1035 WP_252995562.1 type IVa pilus ATPase TapT Machinery gene
  NHF51_RS02710 (NHF51_02710) - 549483..550184 (+) 702 WP_252995563.1 YggS family pyridoxal phosphate-dependent enzyme -
  NHF51_RS02715 (NHF51_02715) proC 550344..551168 (+) 825 WP_252995564.1 pyrroline-5-carboxylate reductase -
  NHF51_RS02720 (NHF51_02720) - 551198..551749 (+) 552 WP_042652090.1 YggT family protein -
  NHF51_RS02725 (NHF51_02725) yggU 551749..552042 (+) 294 WP_218291168.1 DUF167 family protein YggU -
  NHF51_RS02730 (NHF51_02730) - 552062..552481 (+) 420 WP_042652092.1 DUF4426 domain-containing protein -
  NHF51_RS02735 (NHF51_02735) - 552567..553397 (-) 831 WP_252995565.1 CPBP family intramembrane glutamic endopeptidase -
  NHF51_RS02740 (NHF51_02740) - 553593..553889 (-) 297 WP_005332293.1 hypothetical protein -
  NHF51_RS02745 (NHF51_02745) - 553943..554317 (-) 375 WP_042652094.1 YacL family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38263.03 Da        Isoelectric Point: 6.7439

>NTDB_id=701287 NHF51_RS02705 WP_252995562.1 548409..549443(-) (pilT) [Aeromonas encheleia strain SOD01]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVMSLEDLGAPEMFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTRSFANALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGIVAALDAKAKAADPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=701287 NHF51_RS02705 WP_252995562.1 548409..549443(-) (pilT) [Aeromonas encheleia strain SOD01]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAGCTGGAAGAGAACTTCGAGGTGGATTTCTCCTTCGAGGTGCCCAACCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCCGTGTTCCGCACCATCCCCAGCACAGTGATGAGCCTGGA
GGATCTGGGGGCCCCGGAGATGTTTCGCAAGATCGCCGAATTCCCCCGCGGCCTGGTGCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCCACCACCCTGGCAGCCATGGTCGATTACATCAACGACAACTTCCACCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAAAACAAGCGCTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCAGGAGTTTCGCCAA
CGCCCTGCGCTCGGCGCTGCGCGAGGATCCGGACATCATACTGGTGGGGGAGATGCGCGATCTGGAGACCATACGTCTCG
CCATGACCGCCGCCGAGACCGGCCATCTGGTGTTTGGCACCCTGCACACCTCCTCGGCCGCCAAGACCATAGACAGGATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGCTCCATGCTGTCCGAGTCCCTGCGCGCCGTCATCTCCCAGAC
CCTGCTCAAGCGGATCGGCGGGGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCCGCGGTGCGAAACCTCATCC
GGGAAGACAAGATCGCCCAGCTCTACTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGTTGGTCAACCGGGGCATAGTCGCCGCCCTCGACGCCAAGGCCAAGGCGGCCGATCCCAACAGTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.843

99.709

0.776
  pilT Acinetobacter nosocomialis M2

76.453

100

0.765
  pilT Acinetobacter baumannii D1279779

76.453

100

0.765
  pilT Acinetobacter baumannii strain A118

76.453

100

0.765
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.371

97.965

0.738
  pilT Vibrio cholerae strain A1552

75.371

97.965

0.738
  pilT Legionella pneumophila strain Lp02

75.152

95.93

0.721
  pilT Legionella pneumophila strain ERS1305867

75.152

95.93

0.721
  pilT Neisseria meningitidis 8013

68.986

100

0.692
  pilT Neisseria gonorrhoeae MS11

68.696

100

0.689
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.339

99.419

0.52
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404