Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NHF51_RS02700 Genome accession   NZ_CP099717
Coordinates   547274..548383 (-) Length   369 a.a.
NCBI ID   WP_042652086.1    Uniprot ID   A0AAE9MGI0
Organism   Aeromonas encheleia strain SOD01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 542274..553383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHF51_RS02675 (NHF51_02675) - 542404..542631 (+) 228 WP_042652082.1 hypothetical protein -
  NHF51_RS02680 (NHF51_02680) brnQ 542726..543988 (-) 1263 WP_242380592.1 branched-chain amino acid transport system II carrier protein -
  NHF51_RS02685 (NHF51_02685) - 544225..544935 (-) 711 WP_252995560.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NHF51_RS02690 (NHF51_02690) srmB 545064..546284 (+) 1221 WP_042652084.1 ATP-dependent RNA helicase SrmB -
  NHF51_RS02695 (NHF51_02695) yaaA 546469..547242 (-) 774 WP_252995561.1 peroxide stress protein YaaA -
  NHF51_RS02700 (NHF51_02700) pilU 547274..548383 (-) 1110 WP_042652086.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NHF51_RS02705 (NHF51_02705) pilT 548409..549443 (-) 1035 WP_252995562.1 type IVa pilus ATPase TapT Machinery gene
  NHF51_RS02710 (NHF51_02710) - 549483..550184 (+) 702 WP_252995563.1 YggS family pyridoxal phosphate-dependent enzyme -
  NHF51_RS02715 (NHF51_02715) proC 550344..551168 (+) 825 WP_252995564.1 pyrroline-5-carboxylate reductase -
  NHF51_RS02720 (NHF51_02720) - 551198..551749 (+) 552 WP_042652090.1 YggT family protein -
  NHF51_RS02725 (NHF51_02725) yggU 551749..552042 (+) 294 WP_218291168.1 DUF167 family protein YggU -
  NHF51_RS02730 (NHF51_02730) - 552062..552481 (+) 420 WP_042652092.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40868.99 Da        Isoelectric Point: 6.1445

>NTDB_id=701286 NHF51_RS02700 WP_042652086.1 547274..548383(-) (pilU) [Aeromonas encheleia strain SOD01]
MNMDVLLAELVAQKGSDLFITVDAPPTLKVNGRLVSLGDKPLDRPAALALVRESLDAEHFERYLHSREANYAIHREALGR
FRVSAFWQQDMPGMVLRRIETHIPSFGELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHGDGHILTVED
PVEFVHQHGRCLITQREVGIDTESFDVALKNSMRQAPDVILIGEIRNQETMELAIQFAETGHLCLATLHANNANQALDRI
LHLVPEAKHRQFLFDLSFNLRAIVAQQLLPCTDGSRRVAAFEILLNTPLITDIIRKGEMHRLKEVMTKSSELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGSLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=701286 NHF51_RS02700 WP_042652086.1 547274..548383(-) (pilU) [Aeromonas encheleia strain SOD01]
ATGAACATGGATGTTCTGCTGGCTGAACTGGTTGCCCAAAAGGGCTCGGATCTGTTCATCACGGTCGATGCCCCGCCCAC
CCTCAAGGTCAATGGGCGCCTGGTGTCGCTCGGCGATAAACCGCTGGACAGGCCGGCTGCGCTTGCCCTGGTCAGGGAGA
GCCTGGACGCGGAGCACTTCGAGCGCTACCTGCATAGCCGGGAGGCGAACTACGCCATCCATCGCGAGGCGCTGGGACGG
TTCCGGGTCAGCGCCTTCTGGCAGCAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCATATCCCCAGCTTCGG
CGAGCTGGTGCTGCCCCCCATCCTGCAGGAGGTGGCCATGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGCGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGTTATCGCAACCAGCACGGGGACGGTCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCTGCCTGATCACCCAGCGGGAGGTCGGCATCGATACCGAATCCTTCGATGT
GGCGCTCAAGAACTCCATGCGCCAGGCGCCGGACGTCATCCTCATCGGCGAGATCCGCAACCAGGAGACCATGGAGCTGG
CCATCCAGTTTGCCGAGACGGGCCACCTCTGTCTCGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCAGAGGCGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCGCAGCA
GCTGCTGCCCTGCACCGATGGCAGCCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGCGAGATGCATCGGCTCAAGGAGGTGATGACCAAATCCAGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCACGCTGACTCCGCCAACGACCTGCGGCT
GCTGATCAAGCTGTCCGGTCGTGAGCAGCTGGGCGCAGGTTCATTGGACAACGTCACCCTGGACGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.967

99.729

0.588
  pilU Pseudomonas stutzeri DSM 10701

58.12

95.122

0.553
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36