Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ALSL_RS07235 Genome accession   NZ_AP018560
Coordinates   1536754..1537791 (-) Length   345 a.a.
NCBI ID   WP_126537838.1    Uniprot ID   A0A2Z6E501
Organism   Aerosticca soli strain Dysh456     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1531754..1542791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALSL_RS07215 (ALSL_1452) - 1532115..1533041 (+) 927 WP_126537830.1 aspartate carbamoyltransferase catalytic subunit -
  ALSL_RS07220 (ALSL_1453) - 1533111..1534889 (+) 1779 WP_126537832.1 hypothetical protein -
  ALSL_RS07225 (ALSL_1454) - 1535046..1535426 (+) 381 WP_126537834.1 BON domain-containing protein -
  ALSL_RS07230 (ALSL_1455) pilU 1535492..1536661 (-) 1170 WP_126537836.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ALSL_RS07235 (ALSL_1456) pilT 1536754..1537791 (-) 1038 WP_126537838.1 type IV pilus twitching motility protein PilT Machinery gene
  ALSL_RS07240 (ALSL_1457) proC 1538006..1538839 (+) 834 WP_126537840.1 pyrroline-5-carboxylate reductase -
  ALSL_RS07245 (ALSL_1458) - 1538836..1539405 (+) 570 WP_126537842.1 YggT family protein -
  ALSL_RS07250 (ALSL_1459) - 1539402..1542629 (-) 3228 WP_126537844.1 type I restriction endonuclease subunit R -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38456.25 Da        Isoelectric Point: 6.7270

>NTDB_id=70058 ALSL_RS07235 WP_126537838.1 1536754..1537791(-) (pilT) [Aerosticca soli strain Dysh456]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGAPKVFKELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKTEYGHILTIED
PIEFVHTAQKCLINQREVHRDTHGFSEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQCL
QDMVKRGLVTRQQAMEYAKNKDIFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=70058 ALSL_RS07235 WP_126537838.1 1536754..1537791(-) (pilT) [Aerosticca soli strain Dysh456]
ATGGATATTGCCGAGCTGCTGGCTTTTTCGGTCAAGAACAAGGCTTCGGACCTGCATCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGAATCAACCTGCCACCGCTCGATCACAAGCAGGTGCATGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTTCTCGAAGTCGATTTCTCGTTCGAGATTCCGGGCCTGGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAATCGCGGCGCGGGCGCGGTGTTCCGCACCATTCCGAGCGAGGTCCTGACGCTCGA
GGATTTGGGCGCGCCCAAGGTCTTCAAGGAGCTCATCGAGCAGCCGCAGGGTCTGATCCTGGTGACCGGCCCAACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGATCACATCAACAAGACCGAATACGGGCACATCCTCACCATCGAGGAT
CCGATCGAATTCGTGCACACCGCGCAAAAGTGCCTCATCAACCAGCGCGAGGTGCATCGCGACACCCACGGCTTCAGCGA
GGCGCTGCGTTCGGCGCTGCGCGAGGATCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAGACCGGGCATCTGGTGTTCGGCACGCTGCATACGAGCTCGGCCGCCAAGACCGTCGATCGCATC
ATCGACGTGTTTCCCGCGGGCGAAAAACCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCAGCCAGTC
GCTGCTCAAGAAGGTCGGTGGCGGACGCATCGCCGCGCACGAGATCATGGTCGGCATCCCGGCCATCCGCAATCTCATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCCATCCAGACCGGACAGCAATACGGCATGCAGACGCTCGATCAGTGCCTG
CAGGACATGGTCAAACGTGGCCTGGTGACCAGGCAGCAGGCCATGGAATATGCCAAGAACAAGGACATCTTCAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z6E501

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Pseudomonas aeruginosa PAK

79.07

99.71

0.788
  pilT Acinetobacter nosocomialis M2

78.198

99.71

0.78
  pilT Acinetobacter baumannii D1279779

78.198

99.71

0.78
  pilT Acinetobacter baumannii strain A118

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.035

99.71

0.768
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.871

96.522

0.684
  pilT Vibrio cholerae strain A1552

70.871

96.522

0.684
  pilT Neisseria meningitidis 8013

66.472

99.42

0.661
  pilT Neisseria gonorrhoeae MS11

66.181

99.42

0.658
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.525

99.42

0.403
  pilU Vibrio cholerae strain A1552

39.359

99.42

0.391
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment