Detailed information NTG0070057

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ALSL_RS07230
• Genome accession: NZ_AP018560
• Coordinates: 1535492..1536661 (-)
• Length: 389 a.a.
• NCBI ID: WP_126537836.1
• Uniprot ID: A0A2Z6E5C3
• Organism: Aerosticca soli strain Dysh456
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1530492..1541661

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ALSL_RS07205 (ALSL_1450)	-	1531094..1531663 (+)	570	WP_126537826.1	YqgE/AlgH family protein	-
ALSL_RS07210 (ALSL_1451)	ruvX	1531660..1532118 (+)	459	WP_126537828.1	Holliday junction resolvase RuvX	-
ALSL_RS07215 (ALSL_1452)	-	1532115..1533041 (+)	927	WP_126537830.1	aspartate carbamoyltransferase catalytic subunit	-
ALSL_RS07220 (ALSL_1453)	-	1533111..1534889 (+)	1779	WP_126537832.1	hypothetical protein	-
ALSL_RS07225 (ALSL_1454)	-	1535046..1535426 (+)	381	WP_126537834.1	BON domain-containing protein	-
ALSL_RS07230 (ALSL_1455)	pilU	1535492..1536661 (-)	1170	WP_126537836.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ALSL_RS07235 (ALSL_1456)	pilT	1536754..1537791 (-)	1038	WP_126537838.1	type IV pilus twitching motility protein PilT	Machinery gene
ALSL_RS07240 (ALSL_1457)	proC	1538006..1538839 (+)	834	WP_126537840.1	pyrroline-5-carboxylate reductase	-
ALSL_RS07245 (ALSL_1458)	-	1538836..1539405 (+)	570	WP_126537842.1	YggT family protein	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 43437.83 Da
• Isoelectric Point: 6.9647

>NTDB_id=70057 ALSL_RS07230 WP_126537836.1 1535492..1536661(-) (pilU) [Aerosticca soli strain Dysh456]
MSEFDFTAFLKLMVHKKASDLFITAGLAPSMKVQGKIAPITQSPLSAQQARDMVLNVMTPAQREEFEKTHECQFAISAQG
VGRFRVSCFYQRNCVGMVLRRIETKIPTVEELNLPPIVQQLAMTKRGIVILVGATGTGKSTSLAAMIGYRNQHSSGHIIT
IEDPIEYVHRHEGCIITQREIGIDTDSWENALKNTLRQAPDVIMIGEVRTRETMEHAINFAETGHLVLCTLHANNANQAL
DRIIHFFPEDRRQQVFMDLSLNLKGIVAQQLIPSPDGKVRRVAVEVLLGTPLVQDYIRQGEVHKLKDVMKESTNLGMQTF
DQSLVELYHAGEISYEDALRHADSSNEVRLAIKLARGGDARTLSQGLDGVEVEEARDWQGGGAYIRKRP

Nucleotide

• Length: 1170 bp

>NTDB_id=70057 ALSL_RS07230 WP_126537836.1 1535492..1536661(-) (pilU) [Aerosticca soli strain Dysh456]
ATGAGCGAGTTCGATTTCACCGCTTTTTTGAAGCTCATGGTGCACAAGAAGGCTTCCGATCTCTTCATCACCGCCGGCCT
TGCGCCCTCGATGAAGGTCCAGGGCAAGATTGCGCCGATCACACAGAGTCCGCTCTCGGCCCAGCAGGCGCGGGACATGG
TGCTCAATGTGATGACGCCCGCCCAGCGCGAGGAATTTGAAAAGACCCACGAATGCCAGTTCGCCATTTCGGCCCAGGGC
GTCGGACGCTTTCGCGTCTCGTGCTTCTACCAGCGCAACTGCGTAGGCATGGTGTTGCGCCGGATCGAAACCAAGATCCC
GACCGTGGAAGAACTCAACCTGCCGCCGATCGTCCAGCAGCTTGCCATGACCAAACGCGGCATCGTGATCCTGGTCGGTG
CGACGGGTACCGGCAAGTCGACTTCGCTGGCGGCGATGATCGGCTACCGCAACCAGCATTCCAGCGGTCACATCATCACC
ATCGAGGATCCGATCGAGTACGTGCACCGCCACGAGGGCTGCATCATCACCCAGCGCGAAATCGGCATCGATACCGACAG
CTGGGAGAACGCGCTCAAGAACACGCTGCGCCAGGCCCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAACCA
TGGAGCACGCGATCAATTTCGCCGAGACCGGCCATCTGGTGCTGTGTACGCTACATGCCAACAACGCCAATCAGGCGCTC
GATCGCATCATCCATTTCTTTCCCGAGGATCGGCGCCAGCAGGTCTTCATGGATCTGTCGCTGAATCTCAAGGGCATCGT
CGCGCAGCAGCTGATTCCCTCGCCTGACGGCAAGGTGCGCCGGGTCGCGGTGGAAGTGCTGCTGGGCACGCCACTGGTGC
AGGACTACATCCGTCAGGGCGAGGTGCACAAGCTCAAGGACGTGATGAAAGAGTCGACCAACCTCGGCATGCAGACCTTC
GATCAGAGTCTGGTCGAGCTCTATCACGCCGGAGAGATCAGCTACGAGGATGCGCTTCGCCACGCCGACAGTTCCAACGA
AGTGCGCCTCGCCATCAAGCTCGCGCGCGGGGGCGATGCACGGACCCTGTCGCAGGGCTTAGATGGCGTCGAGGTCGAGG
AGGCGCGTGACTGGCAGGGTGGCGGGGCGTATATCAGGAAGCGTCCGTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (108-272)

3D structure

• AlphaFold DB: A0A2Z6E5C3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	67.374	96.915	0.653
pilU	Acinetobacter baylyi ADP1	64.205	90.488	0.581
pilU	Vibrio cholerae strain A1552	54.213	91.517	0.496
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.45	90.231	0.383