Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HPTL_RS08935 Genome accession   NZ_AP018558
Coordinates   1837879..1838919 (+) Length   346 a.a.
NCBI ID   WP_119335667.1    Uniprot ID   A0A2Z6DZN4
Organism   Hydrogenophilus thermoluteolus strain TH-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1832879..1843919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPTL_RS08905 (HPTL_1727) - 1833583..1834908 (+) 1326 WP_119335662.1 dihydroorotase -
  HPTL_RS08910 (HPTL_1728) - 1834898..1835290 (-) 393 WP_119335663.1 NusG domain II-containing protein -
  HPTL_RS08915 (HPTL_1729) - 1835314..1835619 (-) 306 WP_119336157.1 DUF167 domain-containing protein -
  HPTL_RS08920 (HPTL_1730) - 1835621..1836214 (-) 594 WP_119335664.1 YggT family protein -
  HPTL_RS08925 (HPTL_1731) proC 1836218..1837024 (-) 807 WP_119335665.1 pyrroline-5-carboxylate reductase -
  HPTL_RS08930 (HPTL_1732) - 1837015..1837776 (-) 762 WP_119335666.1 YggS family pyridoxal phosphate-dependent enzyme -
  HPTL_RS08935 (HPTL_1733) pilT 1837879..1838919 (+) 1041 WP_119335667.1 type IV pilus twitching motility protein PilT Machinery gene
  HPTL_RS08940 (HPTL_1734) pilU 1838938..1840071 (+) 1134 WP_119335668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HPTL_RS08945 (HPTL_1735) - 1840102..1841346 (-) 1245 WP_119335669.1 class I SAM-dependent RNA methyltransferase -
  HPTL_RS08950 (HPTL_1736) - 1841362..1841739 (-) 378 WP_233467987.1 CopD family protein -
  HPTL_RS08955 (HPTL_1737) - 1841739..1842704 (-) 966 WP_179949076.1 NAD-dependent epimerase/dehydratase family protein -
  HPTL_RS08960 (HPTL_1738) - 1842761..1843780 (+) 1020 WP_119335671.1 2Fe-2S iron-sulfur cluster-binding protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38269.26 Da        Isoelectric Point: 7.0360

>NTDB_id=70048 HPTL_RS08935 WP_119335667.1 1837879..1838919(+) (pilT) [Hydrogenophilus thermoluteolus strain TH-1]
MDIAELLAFAVKNKASDLHLSAGLPPLLRIHGDIRRVNLPALTDADVKALVYDIMTDAQRKAFEAHFEADFSFEIPKLAR
FRVNAFMQSRGMGAVFRTIPSKVLTLEELDAPAVFKTICDQPRGIVLVTGPTGSGKSTTLAAMIDYLNTTQPLHILTIED
PIEFVHEAKKSLINQREVHRDTLSFENALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRAMLSESLRAVISQVLLKRRDGSGRVAAHEIMIGTPAIRNLIRENKIAQMYSIIQTSQALGMQTLDQS
LQQLVARHIVAPDEARAYAVNKDAFR

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=70048 HPTL_RS08935 WP_119335667.1 1837879..1838919(+) (pilT) [Hydrogenophilus thermoluteolus strain TH-1]
ATGGACATCGCCGAACTGCTTGCATTTGCGGTCAAGAACAAAGCATCAGACCTTCACCTTTCGGCGGGGCTACCGCCGTT
GCTGCGCATCCACGGCGACATCCGGCGCGTCAATCTGCCGGCGCTTACCGACGCCGACGTCAAGGCGCTCGTCTATGACA
TCATGACCGACGCGCAACGCAAAGCGTTCGAAGCCCATTTCGAAGCCGACTTCTCGTTTGAGATTCCGAAACTGGCGCGC
TTTCGGGTCAACGCCTTCATGCAAAGCCGCGGCATGGGCGCAGTCTTTCGCACCATTCCCAGCAAAGTGCTCACCCTCGA
AGAGCTCGACGCCCCGGCGGTCTTCAAGACGATCTGCGACCAGCCGCGCGGCATCGTGCTCGTCACCGGCCCCACCGGTT
CTGGGAAATCAACGACGCTGGCGGCGATGATCGACTACCTCAACACCACGCAGCCGCTTCATATCCTCACCATCGAAGAT
CCGATCGAGTTCGTGCACGAAGCGAAAAAATCGCTCATCAACCAGCGTGAGGTCCATCGCGATACCCTCTCATTCGAAAA
CGCGCTGAGGTCGGCATTGCGCGAAGACCCCGACGTGATCCTGGTGGGGGAGATGCGTGACCTCGAAACGATTCGGCTTG
CGCTCACCGCTGCCGAAACCGGCCACCTGGTCTTCGGGACGCTACACACCTCGTCAGCGGCAAAGACGATCGACCGGATC
GTCGACGTCTTTCCTGCTGCCGAAAAAGAGATGGTGCGCGCGATGCTCTCCGAATCGCTGCGCGCGGTGATCTCGCAAGT
ACTCTTGAAGCGGCGCGACGGCAGCGGGCGCGTCGCCGCGCACGAGATCATGATCGGCACGCCCGCGATTCGCAACCTCA
TTCGCGAAAACAAGATCGCGCAGATGTACTCGATCATCCAGACGAGCCAGGCGCTGGGGATGCAGACGCTCGACCAATCG
CTGCAACAACTGGTCGCGCGTCATATCGTTGCCCCCGACGAGGCGCGTGCCTACGCCGTAAACAAAGACGCGTTCCGGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z6DZN4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

73.913

99.711

0.737
  pilT Acinetobacter baumannii D1279779

73.913

99.711

0.737
  pilT Acinetobacter nosocomialis M2

73.623

99.711

0.734
  pilT Pseudomonas aeruginosa PAK

74.631

97.977

0.731
  pilT Pseudomonas stutzeri DSM 10701

73.333

99.711

0.731
  pilT Acinetobacter baylyi ADP1

72.754

99.711

0.725
  pilT Neisseria meningitidis 8013

70.145

99.711

0.699
  pilT Neisseria gonorrhoeae MS11

69.855

99.711

0.697
  pilT Legionella pneumophila strain Lp02

69.565

99.711

0.694
  pilT Legionella pneumophila strain ERS1305867

69.565

99.711

0.694
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.399

0.676
  pilT Vibrio cholerae strain A1552

69.436

97.399

0.676
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.913

99.711

0.538
  pilU Acinetobacter baylyi ADP1

42.075

100

0.422
  pilU Vibrio cholerae strain A1552

43.284

96.821

0.419
  pilU Pseudomonas stutzeri DSM 10701

40.173

100

0.402

Multiple sequence alignment