Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HPTL_RS08940 Genome accession   NZ_AP018558
Coordinates   1838938..1840071 (+) Length   377 a.a.
NCBI ID   WP_119335668.1    Uniprot ID   A0A2Z6E0I6
Organism   Hydrogenophilus thermoluteolus strain TH-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1833938..1845071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPTL_RS08910 (HPTL_1728) - 1834898..1835290 (-) 393 WP_119335663.1 NusG domain II-containing protein -
  HPTL_RS08915 (HPTL_1729) - 1835314..1835619 (-) 306 WP_119336157.1 DUF167 domain-containing protein -
  HPTL_RS08920 (HPTL_1730) - 1835621..1836214 (-) 594 WP_119335664.1 YggT family protein -
  HPTL_RS08925 (HPTL_1731) proC 1836218..1837024 (-) 807 WP_119335665.1 pyrroline-5-carboxylate reductase -
  HPTL_RS08930 (HPTL_1732) - 1837015..1837776 (-) 762 WP_119335666.1 YggS family pyridoxal phosphate-dependent enzyme -
  HPTL_RS08935 (HPTL_1733) pilT 1837879..1838919 (+) 1041 WP_119335667.1 type IV pilus twitching motility protein PilT Machinery gene
  HPTL_RS08940 (HPTL_1734) pilU 1838938..1840071 (+) 1134 WP_119335668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HPTL_RS08945 (HPTL_1735) - 1840102..1841346 (-) 1245 WP_119335669.1 class I SAM-dependent RNA methyltransferase -
  HPTL_RS08950 (HPTL_1736) - 1841362..1841739 (-) 378 WP_233467987.1 CopD family protein -
  HPTL_RS08955 (HPTL_1737) - 1841739..1842704 (-) 966 WP_179949076.1 NAD-dependent epimerase/dehydratase family protein -
  HPTL_RS08960 (HPTL_1738) - 1842761..1843780 (+) 1020 WP_119335671.1 2Fe-2S iron-sulfur cluster-binding protein -
  HPTL_RS08965 (HPTL_1739) - 1843777..1844916 (+) 1140 WP_119335672.1 EAL and HDOD domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42206.69 Da        Isoelectric Point: 6.9142

>NTDB_id=70049 HPTL_RS08940 WP_119335668.1 1838938..1840071(+) (pilU) [Hydrogenophilus thermoluteolus strain TH-1]
MQRDQALKFTTDLLRRVKERNGSDLFIAPDFPPAIKIDGALQPQSNQPLTAAHTLAIARAVMNDRQAAEFEATKECNFAI
SPANIGRFRVNAYRAKGGVSLVFRTIPDTIPTLDSLRLPAILKEVALVKRGLVIFVGGTGTGKSTSLAALIDWRNEHSRG
HIITIEDPIEFLHPHKKCLVSQREIGIDTDSWEIALKNALRQAPDVIMMGEIRDRDTMEYAIAFSETGHLCLATLHANST
NQALDRIINFFPEDRRQQLLMDLSLNLKAIVSQRLLPRASGPGRVAAVEILLNTPLVAELIFKGEIPEIKEVMKRSRELG
MQTFDQALFDLYETGMIRYEDALRYADSVNDLRIQIKLHSKRPDKPEVEVPEGLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=70049 HPTL_RS08940 WP_119335668.1 1838938..1840071(+) (pilU) [Hydrogenophilus thermoluteolus strain TH-1]
ATGCAACGTGACCAAGCGTTGAAATTCACCACCGACCTCCTGCGCCGCGTCAAGGAGCGCAACGGTTCCGACCTCTTCAT
CGCCCCCGATTTCCCGCCAGCGATCAAGATCGACGGGGCGCTGCAACCGCAGTCGAATCAGCCACTCACCGCCGCGCACA
CGCTTGCGATCGCCCGCGCGGTGATGAACGACCGCCAAGCCGCCGAATTCGAAGCCACCAAAGAGTGCAACTTCGCGATC
TCGCCAGCCAACATCGGCCGCTTCCGGGTCAACGCCTACCGCGCCAAAGGGGGGGTTTCGCTCGTCTTTCGCACGATCCC
CGACACCATCCCGACGCTCGACAGTCTTCGGCTCCCCGCCATCCTCAAAGAGGTGGCGCTGGTCAAACGCGGGCTCGTGA
TCTTCGTCGGCGGCACCGGTACCGGGAAATCGACCTCGCTTGCGGCGCTCATCGACTGGCGCAACGAACACAGCCGCGGG
CACATCATCACCATCGAAGATCCGATCGAATTCCTCCATCCCCACAAGAAATGCCTGGTCTCGCAGCGCGAGATCGGGAT
CGACACCGACAGTTGGGAAATCGCGCTCAAAAACGCGCTGCGCCAAGCACCCGACGTGATCATGATGGGCGAAATTCGCG
ACCGCGACACGATGGAGTACGCGATCGCCTTCTCCGAAACAGGGCATCTGTGCCTGGCGACGCTGCACGCGAACAGCACC
AACCAGGCGCTCGATCGCATCATCAACTTCTTCCCCGAAGACCGACGGCAACAGTTGCTGATGGACCTGTCGCTCAACCT
CAAAGCGATCGTCTCACAACGGCTCTTGCCGCGCGCCTCGGGACCAGGGCGCGTCGCTGCGGTTGAGATCCTCCTCAACA
CGCCGCTCGTTGCGGAACTGATCTTCAAAGGGGAGATCCCCGAAATCAAAGAGGTGATGAAACGCTCGCGCGAACTGGGG
ATGCAGACCTTCGATCAAGCGCTCTTCGACCTCTACGAAACTGGGATGATCCGTTACGAAGATGCGCTGCGCTACGCCGA
TTCGGTCAATGACCTGCGCATCCAGATCAAACTCCACAGCAAACGGCCCGACAAGCCCGAAGTGGAAGTCCCAGAAGGGC
TCGATATCGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z6E0I6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.341

96.552

0.573
  pilU Acinetobacter baylyi ADP1

56.863

94.695

0.538
  pilU Vibrio cholerae strain A1552

52.339

90.716

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.192

96.817

0.408
  pilT Legionella pneumophila strain ERS1305867

43.505

87.798

0.382
  pilT Legionella pneumophila strain Lp02

43.505

87.798

0.382
  pilT Neisseria gonorrhoeae MS11

40.828

89.655

0.366
  pilT Neisseria meningitidis 8013

40.828

89.655

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.718

86.472

0.361
  pilT Vibrio cholerae strain A1552

41.718

86.472

0.361

Multiple sequence alignment