Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NG824_RS14325 Genome accession   NZ_CP099534
Coordinates   3296483..3297520 (-) Length   345 a.a.
NCBI ID   WP_010344138.1    Uniprot ID   A0A0J6G068
Organism   Xanthomonas sacchari strain JR3-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3291483..3302520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG824_RS14310 (NG824_14295) - 3292294..3293793 (-) 1500 WP_267092566.1 diguanylate cyclase -
  NG824_RS14315 (NG824_14300) - 3293790..3295067 (-) 1278 WP_267092567.1 WD40 repeat domain-containing protein -
  NG824_RS14320 (NG824_14305) pilU 3295225..3296355 (-) 1131 WP_010344137.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NG824_RS14325 (NG824_14310) pilT 3296483..3297520 (-) 1038 WP_010344138.1 type IV pilus twitching motility protein PilT Machinery gene
  NG824_RS14330 (NG824_14315) - 3297642..3298298 (+) 657 WP_267094198.1 YggS family pyridoxal phosphate-dependent enzyme -
  NG824_RS14335 (NG824_14320) - 3298615..3299643 (+) 1029 WP_318526801.1 hypothetical protein -
  NG824_RS14340 (NG824_14325) proC 3299772..3300617 (+) 846 WP_267092569.1 pyrroline-5-carboxylate reductase -
  NG824_RS14345 (NG824_14330) - 3300760..3301212 (+) 453 WP_267092570.1 DUF4426 domain-containing protein -
  NG824_RS14350 (NG824_14335) soxR 3301458..3301895 (-) 438 WP_010344143.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38578.22 Da        Isoelectric Point: 6.6653

>NTDB_id=700475 NG824_RS14325 WP_010344138.1 3296483..3297520(-) (pilT) [Xanthomonas sacchari strain JR3-14]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQNGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=700475 NG824_RS14325 WP_010344138.1 3296483..3297520(-) (pilT) [Xanthomonas sacchari strain JR3-14]
ATGGATATCGCTGAACTATTGGCGTTCTCGGTCAAGAACAAGGCGTCCGACCTGCACCTGTCCGCGGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTCCGCCGCATCAACATTCCGGCGCTGGACCACAAGCAGGTGCATGCGCTTGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAGGAATTCCTCGAGGTCGACTTCTCCTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATCCCCTCGGAAGTGCTGACCCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAACGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
GGCGCTGCGCTCGGCGTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTCTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCCTGGGAAATCATGGTCGGCACCCCCGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGAACGGCATGCAGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGGTTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J6G068

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.351

98.261

0.78
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397