Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NG824_RS14320 Genome accession   NZ_CP099534
Coordinates   3295225..3296355 (-) Length   376 a.a.
NCBI ID   WP_010344137.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain JR3-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3290225..3301355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG824_RS14300 (NG824_14285) - 3290379..3291095 (-) 717 WP_053069717.1 YitT family protein -
  NG824_RS14305 (NG824_14290) - 3291248..3291844 (-) 597 WP_267092565.1 MFS transporter permease -
  NG824_RS14310 (NG824_14295) - 3292294..3293793 (-) 1500 WP_267092566.1 diguanylate cyclase -
  NG824_RS14315 (NG824_14300) - 3293790..3295067 (-) 1278 WP_267092567.1 WD40 repeat domain-containing protein -
  NG824_RS14320 (NG824_14305) pilU 3295225..3296355 (-) 1131 WP_010344137.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NG824_RS14325 (NG824_14310) pilT 3296483..3297520 (-) 1038 WP_010344138.1 type IV pilus twitching motility protein PilT Machinery gene
  NG824_RS14330 (NG824_14315) - 3297642..3298298 (+) 657 WP_267094198.1 YggS family pyridoxal phosphate-dependent enzyme -
  NG824_RS14335 (NG824_14320) - 3298615..3299643 (+) 1029 WP_318526801.1 hypothetical protein -
  NG824_RS14340 (NG824_14325) proC 3299772..3300617 (+) 846 WP_267092569.1 pyrroline-5-carboxylate reductase -
  NG824_RS14345 (NG824_14330) - 3300760..3301212 (+) 453 WP_267092570.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41906.19 Da        Isoelectric Point: 6.8811

>NTDB_id=700474 NG824_RS14320 WP_010344137.1 3295225..3296355(-) (pilU) [Xanthomonas sacchari strain JR3-14]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMDLSLNLRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=700474 NG824_RS14320 WP_010344137.1 3295225..3296355(-) (pilU) [Xanthomonas sacchari strain JR3-14]
ATGAGCACCATCGACTTCACCTCCTTCCTGAAACTGATGGCGCACCAGAAGGCGTCCGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CAGCGTGGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCTCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGGGTCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGTTGCTGATGGATCTGTCGCTGAACCTGCGCGGCGTGGT
CGCGCAGCAGCTGATCCCGACCCCGGACGGCAAGGGCCGCCGCGTGGCCATGGAGATCATGCTCGGTACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGATGCGCTGCGCCACGCCGATTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCGCGCACCCTGGCGCAGGGCCTGGACGGGGTCGAGATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42

93.085

0.391
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362