Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NG828_RS06980 Genome accession   NZ_CP099530
Coordinates   1657205..1658242 (+) Length   345 a.a.
NCBI ID   WP_267083109.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain YT9-19-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1652205..1663242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG828_RS06955 (NG828_06940) soxR 1652579..1653016 (+) 438 WP_017909921.1 redox-sensitive transcriptional activator SoxR -
  NG828_RS06960 (NG828_06945) - 1653512..1653964 (-) 453 WP_017909920.1 DUF4426 domain-containing protein -
  NG828_RS06965 (NG828_06950) proC 1654108..1654953 (-) 846 WP_267083108.1 pyrroline-5-carboxylate reductase -
  NG828_RS06970 (NG828_06955) - 1655082..1656110 (-) 1029 WP_017913913.1 hypothetical protein -
  NG828_RS06975 (NG828_06960) - 1656427..1657083 (-) 657 WP_230979398.1 YggS family pyridoxal phosphate-dependent enzyme -
  NG828_RS06980 (NG828_06965) pilT 1657205..1658242 (+) 1038 WP_267083109.1 type IV pilus twitching motility protein PilT Machinery gene
  NG828_RS06985 (NG828_06970) pilU 1658370..1659500 (+) 1131 WP_010344137.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NG828_RS06990 (NG828_06975) - 1659506..1660933 (+) 1428 WP_225851698.1 WD40 repeat domain-containing protein -
  NG828_RS06995 (NG828_06980) - 1660930..1662429 (+) 1500 WP_148829668.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38564.20 Da        Isoelectric Point: 6.6653

>NTDB_id=700420 NG828_RS06980 WP_267083109.1 1657205..1658242(+) (pilT) [Xanthomonas sacchari strain YT9-19-2]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQNGMQTLDQHL
QDLVKRSLISRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=700420 NG828_RS06980 WP_267083109.1 1657205..1658242(+) (pilT) [Xanthomonas sacchari strain YT9-19-2]
ATGGATATCGCTGAACTATTGGCGTTCTCGGTCAAGAACAAGGCGTCCGACCTGCACCTGTCCGCGGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTCCGCCGCATCAACATTCCGGCGCTGGACCACAAGCAGGTGCACGCGCTTGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAGGAATTCCTCGAGGTCGACTTCTCCTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCGGAAGTGCTGACCCTGGA
AGACCTCGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAGTACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
GGCGCTGCGCTCGGCGTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCCTGGGAAATCATGGTCGGCACCCCCGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGAACGGCATGCAGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCAGCCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGGTTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.523

99.71

0.803
  pilT Acinetobacter baumannii D1279779

80.523

99.71

0.803
  pilT Acinetobacter baumannii strain A118

80.523

99.71

0.803
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Pseudomonas aeruginosa PAK

79.646

98.261

0.783
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

40.517

100

0.409
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397