Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NG828_RS06985 Genome accession   NZ_CP099530
Coordinates   1658370..1659500 (+) Length   376 a.a.
NCBI ID   WP_010344137.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain YT9-19-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1653370..1664500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG828_RS06960 (NG828_06945) - 1653512..1653964 (-) 453 WP_017909920.1 DUF4426 domain-containing protein -
  NG828_RS06965 (NG828_06950) proC 1654108..1654953 (-) 846 WP_267083108.1 pyrroline-5-carboxylate reductase -
  NG828_RS06970 (NG828_06955) - 1655082..1656110 (-) 1029 WP_017913913.1 hypothetical protein -
  NG828_RS06975 (NG828_06960) - 1656427..1657083 (-) 657 WP_230979398.1 YggS family pyridoxal phosphate-dependent enzyme -
  NG828_RS06980 (NG828_06965) pilT 1657205..1658242 (+) 1038 WP_267083109.1 type IV pilus twitching motility protein PilT Machinery gene
  NG828_RS06985 (NG828_06970) pilU 1658370..1659500 (+) 1131 WP_010344137.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NG828_RS06990 (NG828_06975) - 1659506..1660933 (+) 1428 WP_225851698.1 WD40 repeat domain-containing protein -
  NG828_RS06995 (NG828_06980) - 1660930..1662429 (+) 1500 WP_148829668.1 diguanylate cyclase -
  NG828_RS07000 (NG828_06985) - 1662718..1663314 (+) 597 WP_267083110.1 MFS transporter permease -
  NG828_RS07005 (NG828_06990) - 1663466..1664182 (+) 717 WP_053069717.1 YitT family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41906.19 Da        Isoelectric Point: 6.8811

>NTDB_id=700421 NG828_RS06985 WP_010344137.1 1658370..1659500(+) (pilU) [Xanthomonas sacchari strain YT9-19-2]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMDLSLNLRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=700421 NG828_RS06985 WP_010344137.1 1658370..1659500(+) (pilU) [Xanthomonas sacchari strain YT9-19-2]
ATGAGCACCATCGACTTCACCTCCTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGT
GTGGGGCGCTTCCGCGTCAGTTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CAGCGTGGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACACTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCATGCCATCTCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCGTCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTGAACCTGCGCGGCGTGGT
CGCGCAGCAGCTGATCCCGACCCCGGACGGCAAGGGCCGCCGCGTGGCCATGGAGATCATGCTCGGTACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGATGCGCTGCGCCACGCCGATTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCGCAGGGTGGCGACGCGCGCACCCTGGCGCAGGGCCTGGATGGGGTCGAGATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42

93.085

0.391
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362