Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L1F06_RS22490 Genome accession   NZ_CP099397
Coordinates   4782228..4783262 (-) Length   344 a.a.
NCBI ID   WP_004373763.1    Uniprot ID   -
Organism   Pseudomonas hydrolytica strain DSWY01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4777228..4788262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1F06_RS22470 (L1F06_022470) - 4778385..4778783 (-) 399 WP_012019964.1 TM2 domain-containing protein -
  L1F06_RS22475 (L1F06_022475) - 4778931..4780142 (+) 1212 WP_004373756.1 putative DNA modification/repair radical SAM protein -
  L1F06_RS22480 (L1F06_022480) - 4780142..4781029 (+) 888 WP_129482514.1 TIGR03915 family putative DNA repair protein -
  L1F06_RS22485 (L1F06_022485) pilU 4780995..4782140 (-) 1146 WP_012019966.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L1F06_RS22490 (L1F06_022490) pilT 4782228..4783262 (-) 1035 WP_004373763.1 type IV pilus twitching motility protein PilT Machinery gene
  L1F06_RS22495 (L1F06_022495) - 4783338..4784030 (+) 693 WP_129482513.1 YggS family pyridoxal phosphate-dependent enzyme -
  L1F06_RS22500 (L1F06_022500) proC 4784048..4784869 (+) 822 WP_129482512.1 pyrroline-5-carboxylate reductase -
  L1F06_RS22505 (L1F06_022505) - 4784879..4785472 (+) 594 WP_129482511.1 YggT family protein -
  L1F06_RS22510 (L1F06_022510) - 4785477..4785773 (+) 297 WP_129482510.1 DUF167 domain-containing protein -
  L1F06_RS22515 (L1F06_022515) - 4786010..4787992 (+) 1983 WP_004373774.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37949.62 Da        Isoelectric Point: 6.8889

>NTDB_id=699493 L1F06_RS22490 WP_004373763.1 4782228..4783262(-) (pilT) [Pseudomonas hydrolytica strain DSWY01]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDACL
KTLVSKGLVSRDSAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=699493 L1F06_RS22490 WP_004373763.1 4782228..4783262(-) (pilT) [Pseudomonas hydrolytica strain DSWY01]
ATGGATATCACTGAGCTGCTCGCCTTCAGCGCCAAGCAAGGTGCGTCGGATTTGCACCTCTCGGCGGGTCTGCCGCCGAT
GATCCGCGTCGACGGCGATGTGCGCCGCATCAACCTGCCACCGCTGGATCACAAGCAGGTACACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCCTCGAAGGTGCTGACCATGGA
GGACCTCGGCATGGGCGAGGTGTTCCGCAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCTACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGACTACCTCAACAGCAACAAGTACCACCATATCCTCACCATCGAGGAC
CCCATCGAATTCGTTCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAGGTGCACCGCGACACCCACGGCTTCTCCGA
AGCGCTGCGCTCGGCCCTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGTGTG
GTCGACGTGTTCCCCGCCGAAGAGAAATCCATGGTCCGTTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGTGTCGCCGCTCACGAAATCATGATCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGCGCCCTGGGCATGCAGACCCTCGATGCCTGCCTG
AAGACGCTGGTGTCCAAAGGCCTGGTCAGCCGCGACAGCGCGCGCGAGAAGGCCAAGAGCCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.058

100

0.951
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter nosocomialis M2

85.174

100

0.852
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395