Detailed information NTG0699492

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L1F06_RS22485
• Genome accession: NZ_CP099397
• Coordinates: 4780995..4782140 (-)
• Length: 381 a.a.
• NCBI ID: WP_012019966.1
• Uniprot ID: -
• Organism: Pseudomonas hydrolytica strain DSWY01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4775995..4787140

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L1F06_RS22465 (L1F06_022465)	ntrC	4776827..4778260 (+)	1434	WP_004373752.1	nitrogen regulation protein NR(I)	-
L1F06_RS22470 (L1F06_022470)	-	4778385..4778783 (-)	399	WP_012019964.1	TM2 domain-containing protein	-
L1F06_RS22475 (L1F06_022475)	-	4778931..4780142 (+)	1212	WP_004373756.1	putative DNA modification/repair radical SAM protein	-
L1F06_RS22480 (L1F06_022480)	-	4780142..4781029 (+)	888	WP_129482514.1	TIGR03915 family putative DNA repair protein	-
L1F06_RS22485 (L1F06_022485)	pilU	4780995..4782140 (-)	1146	WP_012019966.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L1F06_RS22490 (L1F06_022490)	pilT	4782228..4783262 (-)	1035	WP_004373763.1	type IV pilus twitching motility protein PilT	Machinery gene
L1F06_RS22495 (L1F06_022495)	-	4783338..4784030 (+)	693	WP_129482513.1	YggS family pyridoxal phosphate-dependent enzyme	-
L1F06_RS22500 (L1F06_022500)	proC	4784048..4784869 (+)	822	WP_129482512.1	pyrroline-5-carboxylate reductase	-
L1F06_RS22505 (L1F06_022505)	-	4784879..4785472 (+)	594	WP_129482511.1	YggT family protein	-
L1F06_RS22510 (L1F06_022510)	-	4785477..4785773 (+)	297	WP_129482510.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42459.72 Da
• Isoelectric Point: 6.5931

>NTDB_id=699492 L1F06_RS22485 WP_012019966.1 4780995..4782140(-) (pilU) [Pseudomonas hydrolytica strain DSWY01]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRIMPVTKNAMSPEQTRETVLGVMNEAQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAQGLALEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=699492 L1F06_RS22485 WP_012019966.1 4780995..4782140(-) (pilU) [Pseudomonas hydrolytica strain DSWY01]
ATGGAATTCGAAAAACTACTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAATGGCCGGATCATGCCGGTGACCAAGAACGCCATGTCGCCGGAGCAGACCCGCGAGACCGTGCTGGGTG
TGATGAACGAAGCGCAGCGCCGCGATTTTGCCGAGAATCACGAGTGCAACTTCGCCATCAGCGCCCGTGGCATCGGTCGT
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCATCGA
TGACCTCAAGCTGCCGGAGATTCTCAAGAAGCTGGCGCTGACCAAGCGTGGCCTGGTGTTGTTCGTCGGTGCCACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGTCAGGCACCGGACGTGATCCTCATCGGTGAGGTGCGTACTCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGCCGACCGGCAGAACCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACCCCGGACGGCAAGGGCCGTCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCTGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGTCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGATCAGGCG
CTGTACAACCTCTACACCCAGGGCGAGATCACCTACGAAGATGCGCTGCTCTACGCCGACTCGGCCAACGACCTGCGCTT
GATGATCAAGCTCGGTTCGGAAACCGACGGCGATCACCTGACCAGCATGGCCCAGGGCCTGGCGCTGGAGGTCAGCGAGG
AAGATCCGGGGCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.388	100	0.924
pilU	Acinetobacter baylyi ADP1	64.146	93.701	0.601
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.452	88.189	0.383