Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFK54_RS10145 Genome accession   NZ_CP099344
Coordinates   2042863..2043843 (-) Length   326 a.a.
NCBI ID   WP_001517057.1    Uniprot ID   A0A7U9DHN1
Organism   Escherichia marmotae strain RHB35-E2-C08     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2037863..2048843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFK54_RS10115 (NFK54_10075) - 2038537..2039034 (+) 498 WP_000856773.1 SprT family zinc-dependent metalloprotease -
  NFK54_RS10120 (NFK54_10080) endA 2039129..2039836 (+) 708 WP_187226609.1 deoxyribonuclease I -
  NFK54_RS10125 (NFK54_10085) rsmE 2039917..2040648 (+) 732 WP_279283600.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFK54_RS10130 (NFK54_10090) gshB 2040661..2041611 (+) 951 WP_000593301.1 glutathione synthase -
  NFK54_RS10135 (NFK54_10095) - 2041720..2042283 (+) 564 WP_010378695.1 YqgE/AlgH family protein -
  NFK54_RS10140 (NFK54_10100) ruvX 2042283..2042699 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  NFK54_RS10145 (NFK54_10105) pilT 2042863..2043843 (-) 981 WP_001517057.1 type IV pilus twitching motility protein PilT Machinery gene
  NFK54_RS10150 (NFK54_10110) yggS 2043861..2044565 (+) 705 WP_016262525.1 pyridoxal phosphate homeostasis protein -
  NFK54_RS10155 (NFK54_10115) yggT 2044583..2045149 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NFK54_RS10160 (NFK54_10120) yggU 2045146..2045436 (+) 291 WP_001277217.1 DUF167 family protein YggU -
  NFK54_RS10165 (NFK54_10125) - 2045444..2046037 (+) 594 WP_001174757.1 XTP/dITP diphosphatase -
  NFK54_RS10170 (NFK54_10130) hemW 2046030..2047166 (+) 1137 WP_000239972.1 radical SAM family heme chaperone HemW -
  NFK54_RS10175 (NFK54_10135) - 2047229..2048236 (-) 1008 WP_279283601.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36041.25 Da        Isoelectric Point: 5.7980

>NTDB_id=698891 NFK54_RS10145 WP_001517057.1 2042863..2043843(-) (pilT) [Escherichia marmotae strain RHB35-E2-C08]
MNMEEIVTLSVKHNVSDLHLCSAWPARWRIRGRMEVAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPAHCPQLEQLGAPPVLPELLNSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698891 NFK54_RS10145 WP_001517057.1 2042863..2043843(-) (pilT) [Escherichia marmotae strain RHB35-E2-C08]
ATGAATATGGAAGAAATTGTGACCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCAGCACG
ATGGCGCATTCGAGGGAGAATGGAGGTCGCGCCGTTTGACGCGCCAGACGTCGAAGAGTTGTTGCGGGAGTGGCTGGATG
ACGATCAACGGGCGATATTGCTTGAGAATGGCCAGCTTGATTTTGCGGTATCGCTGGCTGAAAACCAGCGGCTACGAGGC
AGTGCGTTTGCGCAGCGGCAAGGCATTTCTCTGGCGTTACGGTTGTTACCTGCGCATTGCCCGCAGCTCGAACAACTTGG
CGCGCCGCCGGTATTGCCGGAATTACTCAATAGCGAGAATGGCCTGATTCTGGTGACGGGAGCGACGGGGAGCGGCAAAT
CGACCACACTGGCGGCGATGGTTGGTTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGCTTGATCCAGCAGCGAGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACGCGCGGTGCAGCGCAGGCGGTCGAGCGGCTGGTAGATTCA
TTTCCGGCACAGGAAAAAGATCCCGTGCGTAATCAACTGGCCGGTAGTTTGCGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAGCAGGAAGGGCGCGTGGCGCTGTTTGAATTGTTGATTAACACGCCAGCGGTGGGGAATTTGATACGCGAAGGGA
AAACCCACCAGTTGCCACATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U9DHN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365