Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M5E07_RS13115 Genome accession   NZ_CP098732
Coordinates   2717083..2718201 (+) Length   372 a.a.
NCBI ID   WP_252219835.1    Uniprot ID   A0AAE9RZK9
Organism   Acinetobacter tibetensis strain Y-23     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2712083..2723201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E07_RS13095 (M5E07_13095) - 2712334..2713359 (+) 1026 WP_252219832.1 class I SAM-dependent methyltransferase -
  M5E07_RS13100 (M5E07_13100) - 2713369..2714832 (+) 1464 WP_116760010.1 amino acid permease -
  M5E07_RS13105 (M5E07_13105) - 2715206..2715892 (-) 687 WP_116760008.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5E07_RS13110 (M5E07_13110) pilT 2716016..2717053 (+) 1038 WP_116760006.1 type IV pilus twitching motility protein PilT Machinery gene
  M5E07_RS13115 (M5E07_13115) pilU 2717083..2718201 (+) 1119 WP_252219835.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5E07_RS13120 (M5E07_13120) omp38 2718336..2719352 (-) 1017 WP_116760002.1 outer membrane protein Omp38 -
  M5E07_RS13125 (M5E07_13125) - 2719730..2720248 (+) 519 WP_252219838.1 GspH/FimT family pseudopilin -
  M5E07_RS13130 (M5E07_13130) - 2720350..2721525 (+) 1176 WP_252219840.1 acetyl-CoA C-acyltransferase -
  M5E07_RS13135 (M5E07_13135) - 2721667..2722113 (+) 447 WP_116759996.1 VOC family protein -
  M5E07_RS13140 (M5E07_13140) - 2722202..2722687 (-) 486 WP_252219842.1 DUF934 domain-containing protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41868.21 Da        Isoelectric Point: 6.8894

>NTDB_id=697479 M5E07_RS13115 WP_252219835.1 2717083..2718201(+) (pilU) [Acinetobacter tibetensis strain Y-23]
MDFNDLLNLMIQQKASDLFVTADVEPSMKINGQIIPVAKTKLTGEIVGQLLNSIMSDKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDQPGMVLRRIETKIPTMDELKLPPVLKDLAMTKRGIIIFVGATGTGKSTSLASIIGFRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGDIHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGADRLMNASSHITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=697479 M5E07_RS13115 WP_252219835.1 2717083..2718201(+) (pilU) [Acinetobacter tibetensis strain Y-23]
ATGGACTTTAACGACTTACTCAATCTCATGATTCAACAAAAAGCATCTGACCTTTTCGTCACAGCTGACGTTGAACCATC
CATGAAAATTAACGGTCAAATTATTCCTGTTGCGAAAACCAAGTTAACAGGTGAAATTGTTGGTCAGCTCTTAAATTCAA
TTATGAGCGATAAACAACGCAAAGAATTCGCTGAAACACGCGAATGCAACTTTGCGATTATGAATCGTGATAAAACAGCA
CGTTTCCGTGTCAGTGCTTTTCAACAACGCGACCAACCTGGCATGGTGTTACGTCGTATTGAAACCAAAATTCCAACGAT
GGATGAACTCAAGCTTCCTCCAGTACTCAAAGATTTAGCCATGACCAAACGTGGCATCATTATCTTTGTTGGTGCAACAG
GTACTGGTAAATCAACATCATTGGCTTCAATTATTGGTTTTCGTAATCACAATTCGAAAGGTCATATCATTACCATTGAA
GACCCGATCGAATTTATTCACGAACATGCGGGGTGTATTATCACTCAACGTGAAGTGGGTATCGATACTGATTCCTTTGA
AATTGCATTGAAGAATACTTTGCGTCAAGCACCCGATGTGATTCTGATTGGTGAGATTCGTTCACGTGAGACCATGGACT
ATGCGATTGCCTTCGCAGAAACAGGACATTTAGTTTTTGCAACACTGCATGCCAACAACGCCAACCAAGCCATTGACCGT
ATTATCCACTTCTTTGAAGCGGACCGACACAGTCAACTGTTTATGGATTTATCTTTAAACCTAAAAGCCATGGTTGCACA
GCAACTTATTCCAACCCCAGATGGTAATTCACGTCGCGCTGCGATTGAGATCTTGATTAATTCACCATTAATTTCTGATT
TAATCCGCAAGGGTGATATTCATGAAATTAAAGATTTAATGAAGCGTTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCACTTTATGATTTGTATAAAGCCAAGCAAATCACCTATAAAGATGCACTGAAACACGCCGATTCACCAAATGATTTACG
CTTACAAATCAAACTCTCTGAAGAGGGAGCTGATCGCTTAATGAATGCAAGCTCACATATTACCTTTGATGGTCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.731

0.68
  pilU Vibrio cholerae strain A1552

55.84

94.355

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.033

97.849

0.411
  pilT Legionella pneumophila strain Lp02

41.124

90.86

0.374
  pilT Legionella pneumophila strain ERS1305867

41.124

90.86

0.374
  pilT Pseudomonas aeruginosa PAK

39.884

93.011

0.371
  pilT Acinetobacter nosocomialis M2

39.65

92.204

0.366
  pilT Acinetobacter baumannii D1279779

39.65

92.204

0.366
  pilT Acinetobacter baumannii strain A118

39.65

92.204

0.366
  pilT Acinetobacter baylyi ADP1

39.359

92.204

0.363
  pilT Pseudomonas stutzeri DSM 10701

39.017

93.011

0.363
  pilT Vibrio cholerae strain A1552

38.177

94.355

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

38.177

94.355

0.36
  pilT Neisseria meningitidis 8013

38.728

93.011

0.36
  pilT Neisseria gonorrhoeae MS11

38.728

93.011

0.36