Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5E07_RS13110 Genome accession   NZ_CP098732
Coordinates   2716016..2717053 (+) Length   345 a.a.
NCBI ID   WP_116760006.1    Uniprot ID   -
Organism   Acinetobacter tibetensis strain Y-23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2711016..2722053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E07_RS13095 (M5E07_13095) - 2712334..2713359 (+) 1026 WP_252219832.1 class I SAM-dependent methyltransferase -
  M5E07_RS13100 (M5E07_13100) - 2713369..2714832 (+) 1464 WP_116760010.1 amino acid permease -
  M5E07_RS13105 (M5E07_13105) - 2715206..2715892 (-) 687 WP_116760008.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5E07_RS13110 (M5E07_13110) pilT 2716016..2717053 (+) 1038 WP_116760006.1 type IV pilus twitching motility protein PilT Machinery gene
  M5E07_RS13115 (M5E07_13115) pilU 2717083..2718201 (+) 1119 WP_252219835.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5E07_RS13120 (M5E07_13120) omp38 2718336..2719352 (-) 1017 WP_116760002.1 outer membrane protein Omp38 -
  M5E07_RS13125 (M5E07_13125) - 2719730..2720248 (+) 519 WP_252219838.1 GspH/FimT family pseudopilin -
  M5E07_RS13130 (M5E07_13130) - 2720350..2721525 (+) 1176 WP_252219840.1 acetyl-CoA C-acyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38265.94 Da        Isoelectric Point: 6.6654

>NTDB_id=697478 M5E07_RS13110 WP_116760006.1 2716016..2717053(+) (pilT) [Acinetobacter tibetensis strain Y-23]
MDITELLAFSAKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEQLETDFSFEVPSIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGLGQTFKELCDLPRGLVLVTGPTGSGKSTTLAAMIDYINDKRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMSTLDQSL
KTLVSKGIISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=697478 M5E07_RS13110 WP_116760006.1 2716016..2717053(+) (pilT) [Acinetobacter tibetensis strain Y-23]
ATGGACATTACAGAATTATTGGCTTTTTCTGCAAAAAACGGGGCTTCTGACTTACACTTATCTGCGGGTATGCCACCAAT
GATTCGCGTAGATGGTGAAGTACGTCGCATTAACTTACCAGCTTTAGAACATAAAGAAGTACACAAACTGGTGTACGATA
TTATGAATGACAAACAACGTCGTGACTTTGAAGAACAATTGGAAACTGACTTTTCCTTCGAAGTTCCAAGCATCGCACGT
TTCCGTGTAAACGCATTTAACCAAAACCGCGGCGCTGGCGCAGTATTCCGTACCATTCCTTCAAAAGTTCTTACCATGGA
TGACTTGGGATTAGGACAAACATTTAAAGAGCTTTGTGACTTACCACGTGGTTTAGTTTTAGTCACGGGCCCAACGGGTT
CTGGTAAATCGACCACGTTAGCAGCCATGATCGACTATATTAACGACAAGCGTTATGACCACATTTTAACCGTCGAAGAT
CCAATCGAATTTGTACACCAATCAAAAAAATGCCTGATTAATCAACGTGAAGTACATCGTGATACGCATGGCTTTAACGA
AGCATTACGTTCTGCACTACGTGAAGATCCAGACATTATTTTAGTTGGTGAGATGCGTGACCTTGAAACCATTCGCCTAG
CCCTGACCGCTGCAGAAACAGGTCACTTGGTTTTCGGTACACTGCATACCACTTCTGCGGCCAAAACCATTGACCGTGTG
ATTGATGTATTCCCCGCTGAAGAAAAAGATATGGTTCGTGCCATGCTTTCTGAATCATTACAAGCGGTTATCTCACAAAC
ACTCTTAAAGAAAAATGGTGGTGGCCGTGTCGCCGCGCATGAAATCATGATTGGTATTCCTGCTATTCGTAACCTAATTC
GTGAGAACAAAGTTGCGCAAATGTACTCTGCGATCCAAACAGGTGCTAACTATGGTATGTCCACCTTAGACCAAAGCCTT
AAAACGCTGGTATCTAAAGGTATCATTAGTCCACAAACAGCACGTACTGTTGCCAAACAACCAGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

93.623

100

0.936
  pilT Acinetobacter baumannii D1279779

93.333

100

0.933
  pilT Acinetobacter baumannii strain A118

93.333

100

0.933
  pilT Acinetobacter baylyi ADP1

86.628

99.71

0.864
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.667
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.56

97.391

0.658
  pilT Vibrio cholerae strain A1552

67.56

97.391

0.658
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

42.09

97.101

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.884

100

0.4