Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NDQ71_RS08300 Genome accession   NZ_CP098523
Coordinates   1747315..1748355 (-) Length   346 a.a.
NCBI ID   WP_064667187.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. KG3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1742315..1753355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDQ71_RS08270 (NDQ71_08255) - 1742662..1743135 (+) 474 WP_301562557.1 hypothetical protein -
  NDQ71_RS08275 (NDQ71_08260) rsmE 1743221..1743955 (+) 735 WP_301562558.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NDQ71_RS08280 (NDQ71_08265) gshB 1744010..1744963 (+) 954 WP_301562559.1 glutathione synthase -
  NDQ71_RS08285 (NDQ71_08270) - 1744991..1745548 (+) 558 WP_064667190.1 YqgE/AlgH family protein -
  NDQ71_RS08290 (NDQ71_08275) ruvX 1745585..1746031 (+) 447 WP_064667189.1 Holliday junction resolvase RuvX -
  NDQ71_RS08295 (NDQ71_08280) pilU 1746175..1747305 (-) 1131 WP_064667188.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NDQ71_RS08300 (NDQ71_08285) pilT 1747315..1748355 (-) 1041 WP_064667187.1 type IV pilus twitching motility protein PilT Machinery gene
  NDQ71_RS08305 (NDQ71_08290) - 1748358..1749071 (+) 714 WP_064667186.1 YggS family pyridoxal phosphate-dependent enzyme -
  NDQ71_RS08310 (NDQ71_08295) proC 1749087..1749908 (+) 822 WP_301562560.1 pyrroline-5-carboxylate reductase -
  NDQ71_RS08315 (NDQ71_08300) - 1749966..1750499 (+) 534 WP_064667184.1 YggT family protein -
  NDQ71_RS08320 (NDQ71_08305) - 1750553..1750996 (+) 444 WP_301562561.1 DUF4426 domain-containing protein -
  NDQ71_RS08325 (NDQ71_08310) - 1751000..1751599 (+) 600 WP_301562562.1 XTP/dITP diphosphatase -
  NDQ71_RS08330 (NDQ71_08315) hemW 1751596..1752732 (+) 1137 WP_192541711.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38209.45 Da        Isoelectric Point: 6.3160

>NTDB_id=696601 NDQ71_RS08300 WP_064667187.1 1747315..1748355(-) (pilT) [Pseudoalteromonas sp. KG3]
MDITELLAFSVQHKASDLHLSSGVAPMIRVDGDVRRINIPALENKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLDDLGAPDIFKTISDTPRGLVLVTGPTGSGKSTTLAAMVDYINQHKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKSMIRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIAVPAIRNLIREDKIAQMYSSIQTGASQGMQTMDQCL
TNLVNHGVVTNAEARAKAQDKNNFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=696601 NDQ71_RS08300 WP_064667187.1 1747315..1748355(-) (pilT) [Pseudoalteromonas sp. KG3]
ATGGATATTACCGAATTATTGGCCTTTAGTGTGCAGCACAAAGCATCCGATTTACACTTATCATCAGGGGTTGCGCCAAT
GATCCGTGTTGATGGCGATGTGCGACGTATTAATATACCAGCGCTGGAAAACAAAGATGTGAACAGCCTAGTTTACGACA
TTATGAACGATAATCAACGCAAGGACTACGAACAAAACCTAGAAGTGGATTTTTCGTTTGAAGTGCCAAATTTAGCACGT
TTTCGTGTCAATGCCTTTAACTCTAACCGAGGCCCTGCCGCGGTATTTCGTACAATTCCTAGTGAAGTCCTGACACTTGA
TGATTTAGGCGCGCCAGATATTTTTAAAACTATTTCCGATACGCCACGTGGTCTGGTGTTGGTAACAGGCCCAACTGGCT
CAGGTAAATCAACGACCTTGGCGGCGATGGTCGATTATATTAATCAGCATAAACATCATCATATTTTAACTATCGAAGAT
CCCATTGAGTTTGTACACGACAATAAATTATCACTTATCAACCAACGTGAAGTGCATCGTGATACACATAGTTTTTCTAA
TGCACTGCGCAGTGCTTTACGTGAAGACCCTGATGTTATTTTGGTGGGTGAGTTGCGTGATTTAGAAACTATTCGACTAG
CTATGACCGCAGCTGAAACCGGTCATTTAGTGTTTGGTACTTTGCATACAACTTCAGCGCCAAAAACCATTGACCGTATT
ATCGATGTATTCCCAGGTGAAGAAAAAAGCATGATCCGCTCAATGCTCTCTGAGTCATTGCGGGCGGTTATTTCACAAAC
CCTGCTGAAAAAAATTGGTGGGGGACGAGTCGCAGCCCATGAAATTATGATTGCAGTGCCGGCCATTCGTAACCTGATCC
GCGAAGATAAAATTGCGCAAATGTATTCGTCGATTCAAACTGGCGCATCGCAAGGTATGCAAACCATGGATCAATGTTTA
ACTAATTTAGTAAACCACGGTGTAGTTACCAATGCTGAAGCGCGTGCTAAAGCACAAGATAAAAATAACTTTGGTGGTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.453

99.422

0.76
  pilT Acinetobacter baumannii strain A118

76.453

99.422

0.76
  pilT Acinetobacter nosocomialis M2

76.453

99.422

0.76
  pilT Pseudomonas stutzeri DSM 10701

76.163

99.422

0.757
  pilT Pseudomonas aeruginosa PAK

75.872

99.422

0.754
  pilT Acinetobacter baylyi ADP1

75.581

99.422

0.751
  pilT Legionella pneumophila strain Lp02

72.384

99.422

0.72
  pilT Legionella pneumophila strain ERS1305867

72.384

99.422

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.977

0.699
  pilT Vibrio cholerae strain A1552

71.386

97.977

0.699
  pilT Neisseria meningitidis 8013

69.275

99.711

0.691
  pilT Neisseria gonorrhoeae MS11

68.986

99.711

0.688
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.711

0.52
  pilU Vibrio cholerae strain A1552

40.87

99.711

0.408
  pilU Acinetobacter baylyi ADP1

39.595

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.841

99.711

0.387