Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NDQ71_RS08295 Genome accession   NZ_CP098523
Coordinates   1746175..1747305 (-) Length   376 a.a.
NCBI ID   WP_064667188.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. KG3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1741175..1752305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDQ71_RS08265 (NDQ71_08250) - 1741738..1742631 (+) 894 WP_301562556.1 hypothetical protein -
  NDQ71_RS08270 (NDQ71_08255) - 1742662..1743135 (+) 474 WP_301562557.1 hypothetical protein -
  NDQ71_RS08275 (NDQ71_08260) rsmE 1743221..1743955 (+) 735 WP_301562558.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NDQ71_RS08280 (NDQ71_08265) gshB 1744010..1744963 (+) 954 WP_301562559.1 glutathione synthase -
  NDQ71_RS08285 (NDQ71_08270) - 1744991..1745548 (+) 558 WP_064667190.1 YqgE/AlgH family protein -
  NDQ71_RS08290 (NDQ71_08275) ruvX 1745585..1746031 (+) 447 WP_064667189.1 Holliday junction resolvase RuvX -
  NDQ71_RS08295 (NDQ71_08280) pilU 1746175..1747305 (-) 1131 WP_064667188.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NDQ71_RS08300 (NDQ71_08285) pilT 1747315..1748355 (-) 1041 WP_064667187.1 type IV pilus twitching motility protein PilT Machinery gene
  NDQ71_RS08305 (NDQ71_08290) - 1748358..1749071 (+) 714 WP_064667186.1 YggS family pyridoxal phosphate-dependent enzyme -
  NDQ71_RS08310 (NDQ71_08295) proC 1749087..1749908 (+) 822 WP_301562560.1 pyrroline-5-carboxylate reductase -
  NDQ71_RS08315 (NDQ71_08300) - 1749966..1750499 (+) 534 WP_064667184.1 YggT family protein -
  NDQ71_RS08320 (NDQ71_08305) - 1750553..1750996 (+) 444 WP_301562561.1 DUF4426 domain-containing protein -
  NDQ71_RS08325 (NDQ71_08310) - 1751000..1751599 (+) 600 WP_301562562.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41672.73 Da        Isoelectric Point: 6.0945

>NTDB_id=696600 NDQ71_RS08295 WP_064667188.1 1746175..1747305(-) (pilU) [Pseudoalteromonas sp. KG3]
MTHSLDYFLHLMLEKQGSDLFVSSQLPVSAKINGELIALGDEKISDEESLELVESAMSEKQKAEFHNTKECNFAIATEEG
RFRISAFWQRDCAGMVIRRIVTKIPDVTELGLPSTLTDVIMAKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLSLNLRAIIAQQLVPTADGNGRVAAIEILLNTPIVGELIKKGDIGGIKEAMAKAKDMGMQTFDQ
ALFELYKQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLEPKGQ

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=696600 NDQ71_RS08295 WP_064667188.1 1746175..1747305(-) (pilU) [Pseudoalteromonas sp. KG3]
ATGACACATTCTCTAGATTACTTTTTACATTTGATGCTTGAAAAGCAGGGTTCTGATTTATTTGTTTCAAGTCAATTACC
GGTCAGCGCAAAGATTAATGGTGAGCTGATTGCATTAGGCGACGAAAAAATATCAGACGAAGAGTCGCTTGAGCTAGTTG
AATCTGCGATGAGTGAAAAACAAAAAGCCGAGTTTCATAATACCAAAGAATGCAACTTTGCTATTGCCACCGAAGAAGGG
CGCTTTAGGATCTCAGCATTTTGGCAACGAGATTGTGCCGGCATGGTTATCCGCCGTATTGTTACAAAAATTCCAGATGT
AACTGAGCTTGGCCTACCCTCAACGCTGACCGATGTGATCATGGCAAAGCGGGGGTTGGTGTTATTTGTGGGGGGCACAG
GCACTGGTAAATCAACCTCGTTGGCGGCGTTATTAGGTTATCGTAACCGTAATCAACGTGGCCATATCTTGACTATTGAA
GATCCGATTGAATTTGTTCATGAACACCGTAAAAGTATTATCACTCAGCGTGAAGTCGGTTTAGATACGGAAAGTTTTGA
GTCAGCGCTTAAAAGCTCACTACGCCAAGCTCCTGATGTCATTTTGATTGGTGAAATTCGCTCGCAAGAGACTATGGAAT
ACGCCTTGAGCTTTGCTGAAACCGGGCATTTATGTGTAGCTACGTTACATGCTAATAATGCCAACCAAGCTATCGACCGT
ATCATGCATTTAGTGCCAAAAGAAAAGCACGACAAGCTAAAATATGATCTATCTTTAAATCTACGCGCGATTATTGCCCA
GCAATTAGTGCCTACCGCTGATGGTAATGGCCGGGTCGCGGCAATTGAAATTTTGCTGAACACCCCGATTGTTGGCGAGC
TTATCAAAAAAGGGGATATTGGCGGTATTAAAGAAGCCATGGCTAAAGCGAAAGATATGGGTATGCAAACCTTTGATCAG
GCGCTCTTTGAGCTTTATAAACAGCAGCGAATTAACTACGCTGATGCATTACATCATGCTGATTCACCAAATGATTTACG
TTTGATGATTAAGCTCAGAAACAACGAACAACAAGGCGCTGGTTTTTTACAGGGAGTGACCATTGACGGATTAGAGCCTA
AAGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.599

92.819

0.553
  pilU Acinetobacter baylyi ADP1

55.645

98.936

0.551
  pilU Vibrio cholerae strain A1552

56.164

97.074

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.407

96.809

0.42