Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NDK23_RS05350 Genome accession   NZ_CP098483
Coordinates   1146745..1147782 (+) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas maltophilia strain 142     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1141745..1152782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDK23_RS05325 (NDK23_05325) soxR 1142880..1143314 (+) 435 WP_076739593.1 redox-sensitive transcriptional activator SoxR -
  NDK23_RS05330 (NDK23_05330) - 1143387..1143989 (-) 603 WP_049397188.1 short chain dehydrogenase -
  NDK23_RS05335 (NDK23_05335) - 1144088..1144963 (+) 876 WP_076739592.1 LysR family transcriptional regulator -
  NDK23_RS05340 (NDK23_05340) proC 1145140..1145961 (-) 822 WP_005408367.1 pyrroline-5-carboxylate reductase -
  NDK23_RS05345 (NDK23_05345) - 1145980..1146657 (-) 678 WP_062606619.1 YggS family pyridoxal phosphate-dependent enzyme -
  NDK23_RS05350 (NDK23_05350) pilT 1146745..1147782 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  NDK23_RS05355 (NDK23_05355) pilU 1147931..1149025 (+) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NDK23_RS05360 (NDK23_05360) - 1149194..1150138 (+) 945 WP_012478995.1 IS481-like element ISStma1 family transposase -
  NDK23_RS05365 (NDK23_05365) - 1150262..1151245 (+) 984 WP_049397194.1 aldo/keto reductase -
  NDK23_RS05370 (NDK23_05370) - 1151293..1152192 (-) 900 WP_005412489.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=696189 NDK23_RS05350 WP_004147099.1 1146745..1147782(+) (pilT) [Stenotrophomonas maltophilia strain 142]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=696189 NDK23_RS05350 WP_004147099.1 1146745..1147782(+) (pilT) [Stenotrophomonas maltophilia strain 142]
ATGGATATCGCCGAACTGCTGGCGTTTTCCGTTAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTTCGCCGGATCAACATCCCAGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATTCCATCGCTGGCGCGC
TTCCGAGTGAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAAGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCTGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCATGGCTTCAATGA
AGCACTGCGCTCGGCGCTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAACTTCGTGACCTGGAAACCATCCGCCTGG
CACTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGTTGCGTGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTTGGCGGCGGTCGTACCGCAGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACTGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGAAACCAGGCCCGTGAGTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391