Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NDK23_RS05355 Genome accession   NZ_CP098483
Coordinates   1147931..1149025 (+) Length   364 a.a.
NCBI ID   WP_043033789.1    Uniprot ID   A0A0X3QW38
Organism   Stenotrophomonas maltophilia strain 142     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1149194..1165384 1147931..1149025 flank 169
IScluster/Tn 1149194..1153335 1147931..1149025 flank 169


Gene organization within MGE regions


Location: 1147931..1165384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDK23_RS05355 (NDK23_05355) pilU 1147931..1149025 (+) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NDK23_RS05360 (NDK23_05360) - 1149194..1150138 (+) 945 WP_012478995.1 IS481-like element ISStma1 family transposase -
  NDK23_RS05365 (NDK23_05365) - 1150262..1151245 (+) 984 WP_049397194.1 aldo/keto reductase -
  NDK23_RS05370 (NDK23_05370) - 1151293..1152192 (-) 900 WP_005412489.1 LysR family transcriptional regulator -
  NDK23_RS05375 (NDK23_05375) - 1152421..1153335 (-) 915 WP_012479096.1 IS110-like element ISStma7 family transposase -
  NDK23_RS05380 (NDK23_05380) - 1153741..1153953 (+) 213 WP_076739912.1 DUF4287 domain-containing protein -
  NDK23_RS05385 (NDK23_05385) - 1154064..1154771 (+) 708 WP_014036228.1 YitT family protein -
  NDK23_RS05390 (NDK23_05390) - 1154855..1155400 (-) 546 WP_049395504.1 DNA-3-methyladenine glycosylase I -
  NDK23_RS05395 (NDK23_05395) - 1155397..1157184 (-) 1788 WP_065184125.1 DUF4153 domain-containing protein -
  NDK23_RS05400 (NDK23_05400) - 1157276..1157842 (+) 567 WP_005408377.1 YqgE/AlgH family protein -
  NDK23_RS05405 (NDK23_05405) ruvX 1157835..1158329 (+) 495 WP_005408378.1 Holliday junction resolvase RuvX -
  NDK23_RS05410 (NDK23_05410) - 1158345..1159295 (+) 951 WP_043398325.1 aspartate carbamoyltransferase catalytic subunit -
  NDK23_RS05415 (NDK23_05415) - 1159493..1159984 (+) 492 WP_005408380.1 transposase -
  NDK23_RS05420 (NDK23_05420) - 1160171..1160968 (-) 798 WP_033834838.1 prolyl oligopeptidase family serine peptidase -
  NDK23_RS05425 (NDK23_05425) - 1161039..1161305 (-) 267 WP_005412494.1 hypothetical protein -
  NDK23_RS05430 (NDK23_05430) mgtE 1161368..1162729 (-) 1362 WP_005408383.1 magnesium transporter -
  NDK23_RS05435 (NDK23_05435) ptsP 1162958..1164727 (-) 1770 WP_005408384.1 phosphoenolpyruvate--protein phosphotransferase -
  NDK23_RS05440 (NDK23_05440) - 1164730..1164999 (-) 270 WP_005408385.1 HPr family phosphocarrier protein -
  NDK23_RS05445 (NDK23_05445) - 1164992..1165384 (-) 393 WP_076739911.1 PTS fructose IIA subunit family protein -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40569.61 Da        Isoelectric Point: 6.7680

>NTDB_id=696190 NDK23_RS05355 WP_043033789.1 1147931..1149025(+) (pilU) [Stenotrophomonas maltophilia strain 142]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=696190 NDK23_RS05355 WP_043033789.1 1147931..1149025(+) (pilU) [Stenotrophomonas maltophilia strain 142]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACTGCGGGCATGCCGCCGGCAATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAAT
TCGAGAAGACCCACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTATCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGA
TGATCGGTTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAATTCGTGCACAAGCACGAG
GGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATTGGCGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACC
TGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAATCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAG
ATCAGCTACGAGGACGCGCTGCGTTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCACGCACGCTGTCACAGGGCCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X3QW38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

98.352

0.676
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363