Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NDN13_RS09580 Genome accession   NZ_CP098480
Coordinates   1995488..1996525 (+) Length   345 a.a.
NCBI ID   WP_004655144.1    Uniprot ID   R9AZD4
Organism   Acinetobacter sp. C32I     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1990488..2001525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDN13_RS09560 (NDN13_09555) - 1992405..1993073 (+) 669 WP_251118074.1 HAD-IA family hydrolase -
  NDN13_RS09565 (NDN13_09560) - 1993191..1993586 (-) 396 WP_251118075.1 hypothetical protein -
  NDN13_RS09570 (NDN13_09565) - 1993748..1994488 (-) 741 WP_033134344.1 hypothetical protein -
  NDN13_RS09575 (NDN13_09570) - 1994676..1995362 (-) 687 WP_251118076.1 YggS family pyridoxal phosphate-dependent enzyme -
  NDN13_RS09580 (NDN13_09575) pilT 1995488..1996525 (+) 1038 WP_004655144.1 type IV pilus twitching motility protein PilT Machinery gene
  NDN13_RS09585 (NDN13_09580) pilU 1996550..1997668 (+) 1119 WP_101237599.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NDN13_RS09590 (NDN13_09585) fur 1997765..1998202 (-) 438 WP_004655147.1 ferric iron uptake transcriptional regulator -
  NDN13_RS09595 (NDN13_09590) bamE 1998314..1998712 (+) 399 WP_004655148.1 outer membrane protein assembly factor BamE -
  NDN13_RS09600 (NDN13_09595) - 1998766..1999089 (-) 324 WP_251118077.1 RnfH family protein -
  NDN13_RS09605 (NDN13_09600) - 1999089..2000171 (-) 1083 WP_251118078.1 hypothetical protein -
  NDN13_RS09610 (NDN13_09605) - 2000416..2000871 (-) 456 WP_004655155.1 bacteriohemerythrin -
  NDN13_RS09615 (NDN13_09610) - 2001122..2001466 (-) 345 WP_251118079.1 zinc ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38298.92 Da        Isoelectric Point: 6.5054

>NTDB_id=696147 NDN13_RS09580 WP_004655144.1 1995488..1996525(+) (pilT) [Acinetobacter sp. C32I]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDFEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGSIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGLISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=696147 NDN13_RS09580 WP_004655144.1 1995488..1996525(+) (pilT) [Acinetobacter sp. C32I]
ATGGATATTACAGAACTACTCGCCTTTTCAGTTAAAAACGGTGCTTCCGATTTACACCTTTCAGCGGGTATGCCTCCTAT
GATTCGTGTGGATGGTGAAGTTCGTCGTATTAACTTACCAGCACTTGAACATAAAGATGTACATCGCTTGGTGTACGACA
TTATGAATGATAAACAACGTCGTGACTTTGAAGAAGATTTAGAAACTGACTTTTCATTTGAAGTACCGAATATTGCCCGT
TTCCGTGTCAACGCATTTAACCAGAACCGCGGTGCAGGTGCGGTGTTCCGTACCATTCCATCTAAAGTTTTAACGATGGA
AGATTTGGGCTTAGGTTCAATTTTCAAAGAAATCTGTGATTATCCACGTGGAATTGTACTCGTCACAGGCCCAACGGGTT
CGGGTAAATCCACCACACTTGCAGCGATGATCGACTATATTAACGAAAACCGTTATGACCATATTTTAACGGTTGAAGAT
CCAATTGAATTTGTACACCAATCGAAAAAATGTTTGATTAACCAACGTGAAGTGCATCGTGATACCCACGGCTTTAATGC
AGCACTTCGTTCAGCATTGCGTGAAGACCCTGACATTATTCTGGTCGGTGAGATGCGTGACTTAGAAACCATCCGCTTGG
CACTGACCGCTGCAGAAACAGGCCACTTGGTGTTTGGTACCCTACATACCACCTCTGCGGCGAAAACTATTGACCGTGTC
ATCGACGTATTCCCTGCAGAAGAAAAAGACATGGTGCGTGCCATGCTGTCTGAATCTCTACAAGCCGTGATTTCACAAAC
GCTATTAAAGAAAAATGGCGGTGGTCGTGTGGCTGCACATGAAATCATGATCGGTATTCCTGCGATTCGTAACCTTGTAC
GAGAAAACAAAGTCGCACAAATGTATTCTGCGATCCAAACAGGTGCCAACTACGGTATGACCACTTTGGATCAAAGTCTG
AAAACACTGGTGTCTAAAGGCTTAATTAGCCCACAAACTGCTCGTACTGTTGCAAAACAACCCGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R9AZD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.232

100

0.962
  pilT Acinetobacter baumannii D1279779

95.942

100

0.959
  pilT Acinetobacter baumannii strain A118

95.942

100

0.959
  pilT Acinetobacter baylyi ADP1

87.209

99.71

0.87
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.872

99.71

0.757
  pilT Legionella pneumophila strain ERS1305867

75.872

99.71

0.757
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675
  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394