Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NDN13_RS09585 Genome accession   NZ_CP098480
Coordinates   1996550..1997668 (+) Length   372 a.a.
NCBI ID   WP_101237599.1    Uniprot ID   -
Organism   Acinetobacter sp. C32I     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1991550..2002668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDN13_RS09560 (NDN13_09555) - 1992405..1993073 (+) 669 WP_251118074.1 HAD-IA family hydrolase -
  NDN13_RS09565 (NDN13_09560) - 1993191..1993586 (-) 396 WP_251118075.1 hypothetical protein -
  NDN13_RS09570 (NDN13_09565) - 1993748..1994488 (-) 741 WP_033134344.1 hypothetical protein -
  NDN13_RS09575 (NDN13_09570) - 1994676..1995362 (-) 687 WP_251118076.1 YggS family pyridoxal phosphate-dependent enzyme -
  NDN13_RS09580 (NDN13_09575) pilT 1995488..1996525 (+) 1038 WP_004655144.1 type IV pilus twitching motility protein PilT Machinery gene
  NDN13_RS09585 (NDN13_09580) pilU 1996550..1997668 (+) 1119 WP_101237599.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NDN13_RS09590 (NDN13_09585) fur 1997765..1998202 (-) 438 WP_004655147.1 ferric iron uptake transcriptional regulator -
  NDN13_RS09595 (NDN13_09590) bamE 1998314..1998712 (+) 399 WP_004655148.1 outer membrane protein assembly factor BamE -
  NDN13_RS09600 (NDN13_09595) - 1998766..1999089 (-) 324 WP_251118077.1 RnfH family protein -
  NDN13_RS09605 (NDN13_09600) - 1999089..2000171 (-) 1083 WP_251118078.1 hypothetical protein -
  NDN13_RS09610 (NDN13_09605) - 2000416..2000871 (-) 456 WP_004655155.1 bacteriohemerythrin -
  NDN13_RS09615 (NDN13_09610) - 2001122..2001466 (-) 345 WP_251118079.1 zinc ABC transporter substrate-binding protein -
  NDN13_RS09620 (NDN13_09615) - 2001535..2002383 (-) 849 WP_251118080.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41708.88 Da        Isoelectric Point: 6.3489

>NTDB_id=696148 NDN13_RS09585 WP_101237599.1 1996550..1997668(+) (pilU) [Acinetobacter sp. C32I]
MDFNDLLNLMIQQNASDLFVTADVEPSMKINGQIVPVSKSKLSGEIVGQLVQSIMTDKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRKIETHIPSMDDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMIGFRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNLKALVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLSEEGADQLLSASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=696148 NDN13_RS09585 WP_101237599.1 1996550..1997668(+) (pilU) [Acinetobacter sp. C32I]
ATGGACTTTAATGATTTACTCAATTTGATGATTCAACAAAATGCATCGGACTTATTTGTCACTGCGGATGTTGAACCTTC
AATGAAAATTAACGGGCAGATCGTGCCTGTTTCCAAATCGAAACTTTCAGGGGAAATTGTAGGTCAACTGGTGCAATCCA
TTATGACCGACAAGCAGCGTAAAGAATTTGCAGACACACGTGAATGTAACTTCGCGATCATGAACCGTGAAAAGACGGCG
CGTTTCCGTGTCAGTGCTTTTCAGCAACGTGATATGCCAGGCATGGTACTGCGTAAGATTGAAACGCATATTCCAAGTAT
GGACGACTTGAAGTTACCTGAAGTACTGAAAGAATTGGCGATGACCAAACGTGGCATCATCATCTTTGTTGGTGCAACAG
GGACGGGTAAATCGACGTCTCTTGCGTCAATGATTGGTTTCCGTAATCAAAATTCCAAAGGTCATATCATCACCATTGAA
GACCCGATTGAATTTGTGCATCAACATGCAGGCTGTATCGTGACACAGCGTGAGGTCGGGATTGATACCGATTCATTTGA
GATTGCGCTGAAGAATACACTACGTCAGGCGCCTGACGTAATCTTGATTGGTGAGATTCGTTCACGTGAAACCATGGACT
ATGCCATCGCTTTCGCCGAAACAGGTCACTTGGTGCTTGCCACCCTGCACGCTAACAACGCTAACCAAGCACTGGATCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAGTCAATTGTATATGGATTTGTCGCTGAATTTAAAAGCACTCGTCGCACA
GCAGTTGATTCCAACACCGGATGGTAATTCACGTCGTGCTGCGATTGAGATTTTGATCAATACACCACTTTTGGCGGATT
ATATCCGTAAAGGTGAAATTCATGAGATTAAAGATCTGATGAAACGTTCACGCGAATTGGGTATGCAAACCTTTGACCAA
GCCCTATTCGACCTTTATAAAGCAGGTCAGATTACTTATAAAGATGCCTTGAAACACGCAGACTCGCCAAATGACCTACG
CCTCACCATTAAACTGTCAGAAGAAGGCGCAGACCAGTTATTAAGTGCCAGCCGCAATATCACCTTCGATGGCCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

69.811

99.731

0.696
  pilU Vibrio cholerae strain A1552

53.784

99.462

0.535
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.329

93.011

0.384
  pilT Legionella pneumophila strain Lp02

40.774

90.323

0.368
  pilT Legionella pneumophila strain ERS1305867

40.774

90.323

0.368
  pilT Neisseria meningitidis 8013

40

91.398

0.366
  pilT Neisseria gonorrhoeae MS11

40

91.398

0.366
  pilT Pseudomonas aeruginosa PAK

39.017

93.011

0.363
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36