Detailed information NTG0069409

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ECTOK1_RS15785
• Genome accession: NZ_AP018395
• Coordinates: 3186565..3187545 (-)
• Length: 326 a.a.
• NCBI ID: WP_024172496.1
• Uniprot ID: -
• Organism: Escherichia coli strain TOK-001
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3181565..3192545

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECTOK1_RS15750 (ECTOK1_C30050)	yggI	3182220..3182717 (+)	498	WP_001685469.1	SprT family zinc-dependent metalloprotease	-
ECTOK1_RS15755 (ECTOK1_C30060)	endA	3182812..3183519 (+)	708	WP_000286492.1	deoxyribonuclease I	-
ECTOK1_RS15760 (ECTOK1_C30070)	rsmE	3183599..3184330 (+)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
ECTOK1_RS15765 (ECTOK1_C30080)	gshB	3184343..3185293 (+)	951	WP_000593273.1	glutathione synthase	-
ECTOK1_RS15770 (ECTOK1_C30090)	yqgE	3185402..3185965 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
ECTOK1_RS15775 (ECTOK1_C30100)	ruvX	3185965..3186381 (+)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
ECTOK1_RS15780	-	3186388..3186516 (-)	129	WP_001278270.1	hypothetical protein	-
ECTOK1_RS15785 (ECTOK1_C30110)	pilT	3186565..3187545 (-)	981	WP_024172496.1	type IV pilus twitching motility protein PilT	Machinery gene
ECTOK1_RS15790 (ECTOK1_C30120)	yggS	3187563..3188267 (+)	705	WP_001411120.1	pyridoxal phosphate homeostasis protein	-
ECTOK1_RS15795 (ECTOK1_C30130)	yggT	3188285..3188851 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
ECTOK1_RS15800 (ECTOK1_C30140)	yggU	3188848..3189138 (+)	291	WP_001277222.1	DUF167 family protein YggU	-
ECTOK1_RS15805 (ECTOK1_C30150)	rdgB	3189146..3189739 (+)	594	WP_001174735.1	XTP/dITP diphosphatase	-
ECTOK1_RS15810 (ECTOK1_C30160)	hemW	3189732..3190868 (+)	1137	WP_001411121.1	radical SAM family heme chaperone HemW	-
ECTOK1_RS15815 (ECTOK1_C30170)	yggM	3190937..3191944 (-)	1008	WP_000745244.1	DUF1202 family protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35956.16 Da
• Isoelectric Point: 5.7654

>NTDB_id=69409 ECTOK1_RS15785 WP_024172496.1 3186565..3187545(-) (pilT) [Escherichia coli strain TOK-001]
MNMEEIVTLSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=69409 ECTOK1_RS15785 WP_024172496.1 3186565..3187545(-) (pilT) [Escherichia coli strain TOK-001]
ATGAATATGGAAGAAATTGTGACCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCATGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.93	100	0.491
pilT	Vibrio cholerae strain A1552	48.93	100	0.491
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	45.872	100	0.46
pilT	Acinetobacter baylyi ADP1	45.566	100	0.457
pilT	Pseudomonas aeruginosa PAK	45.26	100	0.454
pilT	Legionella pneumophila strain ERS1305867	45.597	97.546	0.445
pilT	Legionella pneumophila strain Lp02	45.597	97.546	0.445
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.062	98.773	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	37.048	100	0.377
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362