Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NAF16_RS12300 Genome accession   NZ_CP098021
Coordinates   2611841..2612878 (-) Length   345 a.a.
NCBI ID   WP_024374563.1    Uniprot ID   -
Organism   Vibrio fluvialis strain V13     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2606841..2617878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAF16_RS12270 (NAF16_12245) - 2607071..2607772 (+) 702 WP_032080466.1 endonuclease -
  NAF16_RS12275 (NAF16_12250) rsmE 2607869..2608600 (+) 732 WP_323694815.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NAF16_RS12280 (NAF16_12255) gshB 2608613..2609569 (+) 957 WP_024374566.1 glutathione synthase -
  NAF16_RS12285 (NAF16_12260) - 2609609..2610172 (+) 564 WP_032080468.1 YqgE/AlgH family protein -
  NAF16_RS12290 (NAF16_12265) ruvX 2610234..2610659 (+) 426 WP_044366735.1 Holliday junction resolvase RuvX -
  NAF16_RS12295 (NAF16_12270) pilU 2610723..2611829 (-) 1107 WP_323694816.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NAF16_RS12300 (NAF16_12275) pilT 2611841..2612878 (-) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  NAF16_RS12305 (NAF16_12280) - 2612904..2613611 (+) 708 WP_154184781.1 YggS family pyridoxal phosphate-dependent enzyme -
  NAF16_RS12310 (NAF16_12285) proC 2613648..2614466 (+) 819 WP_061056912.1 pyrroline-5-carboxylate reductase -
  NAF16_RS12315 (NAF16_12290) - 2614518..2615075 (+) 558 WP_024374560.1 YggT family protein -
  NAF16_RS12320 (NAF16_12295) yggU 2615075..2615365 (+) 291 WP_024374559.1 DUF167 family protein YggU -
  NAF16_RS12325 (NAF16_12300) - 2615425..2615856 (+) 432 WP_032080472.1 DUF4426 domain-containing protein -
  NAF16_RS12330 (NAF16_12305) - 2615880..2616479 (+) 600 WP_024374557.1 XTP/dITP diphosphatase -
  NAF16_RS12335 (NAF16_12310) hemW 2616479..2617651 (+) 1173 WP_323695200.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38227.83 Da        Isoelectric Point: 6.5038

>NTDB_id=693563 NAF16_RS12300 WP_024374563.1 2611841..2612878(-) (pilT) [Vibrio fluvialis strain V13]
MDISELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLIFEIMNDAQRSEYEEKLEVDFSFALPDIGR
FRVNAFHQSRGASAVFRTIPTVIPTLDQLEAPEIFHKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINATHNKHVLTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRLGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGASHGMQTMEQHA
KQLIAQGLVDPAEVQKKIELEMSAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=693563 NAF16_RS12300 WP_024374563.1 2611841..2612878(-) (pilT) [Vibrio fluvialis strain V13]
ATGGATATCTCTGAGTTACTGGAATTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCGGGTGTTCCTCCTAT
GGTACGCATCGATGGTGAAGTGAGAAAGCTTGGCGTACCTGCGTTTACACACGCTGATGTGCATCGATTAATTTTTGAAA
TTATGAATGATGCCCAGCGCAGTGAGTACGAAGAAAAGCTGGAAGTGGACTTTTCCTTTGCGTTACCGGACATCGGCCGA
TTTCGTGTCAACGCTTTCCATCAGTCGCGCGGCGCTTCTGCGGTATTTCGTACCATTCCGACCGTGATCCCGACTCTGGA
CCAGCTTGAGGCTCCGGAAATTTTCCACAAAATCGCCAATTACGAAAAAGGTTTGGTGCTGGTGACCGGACCGACCGGCT
CGGGTAAATCAACCACGTTAGCGGCGATGGTCGATTACATCAACGCAACCCATAACAAGCATGTGCTGACTATCGAAGAT
CCGATTGAGTTTGTGCACAGCAACAACAAATGCTTGATCAACCAGCGTGAAGTACACCGTGATACCCACAGTTTTAAAAA
CGCGCTGCGCAGCGCATTGCGTGAAGATCCGGATGTGATTCTGGTCGGTGAGCTGCGCGATCAGGAAACCATCAGCTTAG
CGCTGACGGCCGCGGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCAGTTCGGCGGCGAAAACCATTGACCGCATC
ATCGATGTGTTCCCGGGCAGCGACAAGGACATGGTACGTTCAATGTTGTCAGAATCGCTGCGTGCGGTGATTGCGCAAAA
ACTGCTCAAACGTCTGGGTGGCGGGCGAGTGGCGTGTCATGAAATCATGTTGGCGACGCCTGCTATCCGTAACCTGATTC
GCGAAGATAAAGTCGCGCAGATGTATTCGATCATTCAGACTGGCGCCTCCCATGGAATGCAGACTATGGAGCAGCATGCC
AAACAGTTGATCGCGCAAGGGCTGGTGGATCCGGCAGAAGTGCAGAAGAAAATTGAACTGGAAATGTCCGCGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.754

100

0.928
  pilT Vibrio cholerae strain A1552

92.754

100

0.928
  pilT Acinetobacter baumannii D1279779

71.601

95.942

0.687
  pilT Acinetobacter baumannii strain A118

71.601

95.942

0.687
  pilT Acinetobacter nosocomialis M2

71.299

95.942

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.059

98.551

0.661
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

62.059

98.551

0.612
  pilT Neisseria gonorrhoeae MS11

61.765

98.551

0.609
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.424

95.652

0.501
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.867

96.232

0.403
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374