Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NAF16_RS12295 Genome accession   NZ_CP098021
Coordinates   2610723..2611829 (-) Length   368 a.a.
NCBI ID   WP_323694816.1    Uniprot ID   -
Organism   Vibrio fluvialis strain V13     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2605723..2616829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAF16_RS12265 (NAF16_12240) - 2606452..2606952 (+) 501 WP_172557955.1 SprT family zinc-dependent metalloprotease -
  NAF16_RS12270 (NAF16_12245) - 2607071..2607772 (+) 702 WP_032080466.1 endonuclease -
  NAF16_RS12275 (NAF16_12250) rsmE 2607869..2608600 (+) 732 WP_323694815.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NAF16_RS12280 (NAF16_12255) gshB 2608613..2609569 (+) 957 WP_024374566.1 glutathione synthase -
  NAF16_RS12285 (NAF16_12260) - 2609609..2610172 (+) 564 WP_032080468.1 YqgE/AlgH family protein -
  NAF16_RS12290 (NAF16_12265) ruvX 2610234..2610659 (+) 426 WP_044366735.1 Holliday junction resolvase RuvX -
  NAF16_RS12295 (NAF16_12270) pilU 2610723..2611829 (-) 1107 WP_323694816.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NAF16_RS12300 (NAF16_12275) pilT 2611841..2612878 (-) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  NAF16_RS12305 (NAF16_12280) - 2612904..2613611 (+) 708 WP_154184781.1 YggS family pyridoxal phosphate-dependent enzyme -
  NAF16_RS12310 (NAF16_12285) proC 2613648..2614466 (+) 819 WP_061056912.1 pyrroline-5-carboxylate reductase -
  NAF16_RS12315 (NAF16_12290) - 2614518..2615075 (+) 558 WP_024374560.1 YggT family protein -
  NAF16_RS12320 (NAF16_12295) yggU 2615075..2615365 (+) 291 WP_024374559.1 DUF167 family protein YggU -
  NAF16_RS12325 (NAF16_12300) - 2615425..2615856 (+) 432 WP_032080472.1 DUF4426 domain-containing protein -
  NAF16_RS12330 (NAF16_12305) - 2615880..2616479 (+) 600 WP_024374557.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41200.13 Da        Isoelectric Point: 6.1909

>NTDB_id=693562 NAF16_RS12295 WP_323694816.1 2610723..2611829(-) (pilU) [Vibrio fluvialis strain V13]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAQGEKLDEAQVNALLAAMMDEARHADYLATREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPAVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGDLHELKSTMAKSKEVGMQTFDQALY
QLVVENKISEQDALHCADSANDLRLMLKTQRGDVSSSGSLDHVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=693562 NAF16_RS12295 WP_323694816.1 2610723..2611829(-) (pilU) [Vibrio fluvialis strain V13]
ATGCAACTCGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCAGATCTATATATTACTGTGGGCGCACCCGTGCT
GTACCGGGTCGATGGTGAACTGCGCGCGCAGGGTGAGAAGCTGGATGAAGCGCAGGTCAACGCGCTGCTGGCTGCGATGA
TGGATGAGGCGCGCCATGCGGACTATCTGGCGACGCGTGAAGCTAACTTTGCCATCGTGCGTGATTTGGGGCGTTTTCGC
GTCAGCGCTTTTTTTCAGCGCGAGCTACCTGGTGCGGTGATTCGCCGCATCGAAACGCAGATTCCGACCTTTGATGAACT
CAAACTGCCAGCGGTGCTGAAAGATCTCTCGATTGCCAAACGCGGTTTAGTGTTGGTGGTGGGGGCGACAGGATCGGGCA
AATCGACCACCATGGCCGCGATGACTGGCTATCGCAACACGCACCGCAGCGGGCATATTCTCACCGTCGAAGATCCGATT
GAATTTGTCCATGAGCACCAGCGCTGCATCGTGACGCAGCGCGAAGTGGGATTGGACACCGAAAGCTATGAAGTGGCGCT
GAAGAACTCGCTACGCCAGGCGCCGGATATGATTTTGATCGGAGAGATCCGCAGCCGTGAAACCATGGAGTACGCAATGA
CCTTCGCCGAAACCGGTCACTTGTGCATGGCAACTCTGCACGCCAACAACGCCAACCAAGCGCTTGAGCGTATTTTGCAT
CTGGTGCCGAAAGAGCAGCGTGAACAATTTCTGTTTGACCTGTCGATGAACCTCAAAGGTGTTGTCGGTCAGCAACTGAT
CCGCGATAAAAACGGGCAGGGGCGCCACGGCGTGTTTGAAGTGTTGCTCAACAGTCCACGGGTGTCGGACATCATCCGAC
GCGGCGATTTGCATGAGCTGAAAAGCACCATGGCGAAATCGAAAGAGGTCGGCATGCAAACCTTTGATCAGGCGTTGTAT
CAGTTGGTGGTCGAGAACAAGATCAGCGAACAGGATGCGCTGCACTGTGCCGATTCGGCCAACGATCTGCGCTTGATGCT
CAAGACTCAGCGGGGTGATGTGAGCAGCAGTGGTTCACTGGATCATGTGCAAATCGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.598

100

0.856
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44

88.315

0.389
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Pseudomonas aeruginosa PAK

41.194

91.033

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.701

91.033

0.361