Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CKCBHOJB_RS02480 Genome accession   NZ_CP097870
Coordinates   564798..565841 (-) Length   347 a.a.
NCBI ID   WP_281050461.1    Uniprot ID   -
Organism   Thauera sp. GDN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 559798..570841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKCBHOJB_RS02455 (CKCBHOJB_00492) fdh3B 560349..560987 (+) 639 WP_281050456.1 formate dehydrogenase FDH3 subunit beta -
  CKCBHOJB_RS02460 (CKCBHOJB_00493) - 560984..561220 (+) 237 WP_281050457.1 hypothetical protein -
  CKCBHOJB_RS02465 (CKCBHOJB_00494) - 561231..562253 (+) 1023 WP_281050458.1 formate dehydrogenase subunit gamma -
  CKCBHOJB_RS02470 (CKCBHOJB_00495) - 562453..563616 (+) 1164 WP_281050459.1 THUMP domain-containing protein -
  CKCBHOJB_RS02475 (CKCBHOJB_00496) pilU 563645..564778 (-) 1134 WP_281050460.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CKCBHOJB_RS02480 (CKCBHOJB_00497) pilT 564798..565841 (-) 1044 WP_281050461.1 type IV pilus twitching motility protein PilT Machinery gene
  CKCBHOJB_RS02485 (CKCBHOJB_00498) - 565954..566655 (+) 702 WP_281050462.1 YggS family pyridoxal phosphate-dependent enzyme -
  CKCBHOJB_RS02490 (CKCBHOJB_00499) proC 566721..567545 (+) 825 WP_281050463.1 pyrroline-5-carboxylate reductase -
  CKCBHOJB_RS02495 (CKCBHOJB_00500) - 567549..568130 (+) 582 WP_281050464.1 YggT family protein -
  CKCBHOJB_RS02500 (CKCBHOJB_00501) - 568266..569543 (-) 1278 WP_281050465.1 dihydroorotase -
  CKCBHOJB_RS02505 (CKCBHOJB_00502) - 569577..570536 (-) 960 WP_281050466.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38601.26 Da        Isoelectric Point: 7.0160

>NTDB_id=693165 CKCBHOJB_RS02480 WP_281050461.1 564798..565841(-) (pilT) [Thauera sp. GDN1]
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKEVHAMVYDIMNDGQRKQFEETWECDFSFAVPNLAR
FRVNAFNQNRGAAAVFRTIPSKVLTLEELNCPKIFKEIANQPRGIVLVTGPTGSGKSTTLAAMIDYVNENEYGHILTVED
PIEFVHESKRCLINQREVHRDTMSFNNALRAALREDPDVVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIRENKVAQMYSSIQTGQNVGMQTLDQC
LADLVRRNVVSATEARIRAQNKDNFAA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=693165 CKCBHOJB_RS02480 WP_281050461.1 564798..565841(-) (pilT) [Thauera sp. GDN1]
ATGGACATCACCGAACTGCTCGCCTTCGCGGTCAAGAACAAGGCCTCCGACCTTCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGGGTCCATGGCGACGTGCGCCGCATCAACCTGCCACCGCTGGAGCACAAGGAAGTGCACGCGATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGCAGTTCGAGGAAACCTGGGAGTGCGACTTCTCCTTCGCCGTGCCCAACCTAGCCCGC
TTCCGTGTCAACGCGTTCAACCAGAACCGCGGCGCGGCGGCGGTGTTCCGCACCATTCCGTCCAAGGTGCTGACGCTGGA
AGAGCTCAACTGCCCGAAGATCTTCAAGGAGATCGCCAACCAGCCGCGCGGCATCGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACGTGAACGAAAACGAGTACGGCCACATCCTGACCGTCGAGGAT
CCGATCGAATTCGTGCATGAATCCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCATGTCCTTCAACAA
CGCGCTGCGCGCTGCGCTGCGCGAGGACCCGGACGTGGTGCTGGTGGGCGAGATGCGCGACCTCGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
GTCGACGTCTTTCCGGCGGCGGAGAAGGACATGGTGCGCTCGATGCTGTCGGAGTCGCTGCGCGCGGTGATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGCTCGGGCCGGGTGGCGGCGCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGGTCGCGCAGATGTACTCCTCGATCCAGACCGGCCAGAACGTCGGCATGCAGACGCTGGACCAGTGT
CTGGCCGATCTGGTGCGGCGCAACGTGGTGTCGGCGACCGAGGCGCGCATCCGGGCGCAGAACAAGGACAATTTCGCGGC
GTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.42

99.424

0.79
  pilT Acinetobacter baumannii D1279779

79.42

99.424

0.79
  pilT Acinetobacter baumannii strain A118

79.42

99.424

0.79
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Acinetobacter baylyi ADP1

76.522

99.424

0.761
  pilT Legionella pneumophila strain Lp02

73.623

99.424

0.732
  pilT Legionella pneumophila strain ERS1305867

73.623

99.424

0.732
  pilT Neisseria meningitidis 8013

70.809

99.712

0.706
  pilT Neisseria gonorrhoeae MS11

70.52

99.712

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

96.83

0.671
  pilT Vibrio cholerae strain A1552

69.345

96.83

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.179

99.712

0.53
  pilU Acinetobacter baylyi ADP1

41.667

100

0.418
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.87

99.424

0.406