Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CKCBHOJB_RS02475 Genome accession   NZ_CP097870
Coordinates   563645..564778 (-) Length   377 a.a.
NCBI ID   WP_281050460.1    Uniprot ID   -
Organism   Thauera sp. GDN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 558645..569778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKCBHOJB_RS02455 (CKCBHOJB_00492) fdh3B 560349..560987 (+) 639 WP_281050456.1 formate dehydrogenase FDH3 subunit beta -
  CKCBHOJB_RS02460 (CKCBHOJB_00493) - 560984..561220 (+) 237 WP_281050457.1 hypothetical protein -
  CKCBHOJB_RS02465 (CKCBHOJB_00494) - 561231..562253 (+) 1023 WP_281050458.1 formate dehydrogenase subunit gamma -
  CKCBHOJB_RS02470 (CKCBHOJB_00495) - 562453..563616 (+) 1164 WP_281050459.1 THUMP domain-containing protein -
  CKCBHOJB_RS02475 (CKCBHOJB_00496) pilU 563645..564778 (-) 1134 WP_281050460.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CKCBHOJB_RS02480 (CKCBHOJB_00497) pilT 564798..565841 (-) 1044 WP_281050461.1 type IV pilus twitching motility protein PilT Machinery gene
  CKCBHOJB_RS02485 (CKCBHOJB_00498) - 565954..566655 (+) 702 WP_281050462.1 YggS family pyridoxal phosphate-dependent enzyme -
  CKCBHOJB_RS02490 (CKCBHOJB_00499) proC 566721..567545 (+) 825 WP_281050463.1 pyrroline-5-carboxylate reductase -
  CKCBHOJB_RS02495 (CKCBHOJB_00500) - 567549..568130 (+) 582 WP_281050464.1 YggT family protein -
  CKCBHOJB_RS02500 (CKCBHOJB_00501) - 568266..569543 (-) 1278 WP_281050465.1 dihydroorotase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42210.64 Da        Isoelectric Point: 6.4002

>NTDB_id=693164 CKCBHOJB_RS02475 WP_281050460.1 563645..564778(-) (pilU) [Thauera sp. GDN1]
MERDQALRFMHDLLRLMLQKNGSDLFITTGFPPAIKIDGKIVPQSNQALQPQHTAELARAVMNDRQAAEFEARKECNFGI
SPAGIGRFRANAFVQQGRVGLVLRTIPQKIPNFDELGLPPVLTEIALAKRGLVIFVGGTGTGKTTSLASMVDYRNENTYG
HIITVEDPIEFVHAHKNCIVTQREVGIDTESWEAALKNTLRQAPDVILMGEIRDRDTMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEERRTQLLMDLSLNLRAMISQRLLPLKDRKGRVPAVEVMLNSPLIADLIFKGNVPEIKEIMKRSRELG
MQTFDQSLFELYESGLISYEDALRNADSINDLRLQIKLYSKLGEQDVGGSLKNLDIL

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=693164 CKCBHOJB_RS02475 WP_281050460.1 563645..564778(-) (pilU) [Thauera sp. GDN1]
ATGGAACGCGACCAGGCCCTCAGGTTCATGCACGATCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCCGACCTCTTCAT
CACCACCGGCTTTCCGCCCGCGATCAAGATCGACGGCAAGATCGTGCCGCAGTCCAACCAGGCCCTGCAGCCGCAGCACA
CCGCGGAACTGGCGCGTGCGGTCATGAACGACCGCCAGGCGGCGGAATTCGAGGCGCGCAAGGAGTGCAACTTCGGCATC
TCACCCGCCGGCATCGGCCGTTTCCGCGCCAATGCCTTCGTGCAGCAGGGCCGCGTCGGCCTGGTGCTGCGCACCATCCC
GCAGAAGATCCCGAACTTCGACGAGCTCGGCCTGCCGCCGGTGCTGACCGAGATCGCGCTCGCCAAGCGCGGGCTGGTGA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACGACCTCGCTGGCGTCGATGGTCGACTACCGCAACGAGAACACCTACGGC
CACATCATCACCGTCGAGGACCCGATCGAGTTCGTGCACGCGCACAAGAACTGCATCGTCACCCAGCGCGAGGTCGGCAT
CGACACCGAGTCCTGGGAGGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGACACCATGGACTACGCGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCCATCGACCGCATCATCAACTTCTTCCCGGAAGAGCGCCGCACGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCGATGATCTCGCAGCGTCTGCTGCCACTGAAGGACCGCAAGGGCCGGGTGCCGGCGGTCGAGGTGATGCTGAACT
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCAACGTGCCCGAGATCAAGGAGATCATGAAGCGCTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGAGCTCTACGAGAGCGGACTGATCAGCTACGAGGACGCGCTGCGCAACGCCGA
CTCGATCAACGACCTGCGCCTGCAGATCAAGCTGTACAGCAAGCTCGGCGAGCAGGACGTCGGCGGCAGCCTGAAGAACC
TCGACATCCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.573

0.592
  pilU Acinetobacter baylyi ADP1

58.621

92.308

0.541
  pilU Vibrio cholerae strain A1552

55.556

95.491

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.075

88.859

0.401
  pilT Legionella pneumophila strain Lp02

43.505

87.798

0.382
  pilT Legionella pneumophila strain ERS1305867

43.505

87.798

0.382
  pilT Pseudomonas stutzeri DSM 10701

42.687

88.859

0.379
  pilT Acinetobacter baylyi ADP1

42.433

89.39

0.379
  pilT Pseudomonas aeruginosa PAK

42.388

88.859

0.377
  pilT Acinetobacter baumannii D1279779

42.9

87.798

0.377
  pilT Acinetobacter nosocomialis M2

42.9

87.798

0.377
  pilT Acinetobacter baumannii strain A118

42.9

87.798

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.212

87.533

0.361
  pilT Vibrio cholerae strain A1552

41.212

87.533

0.361