Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M8C01_RS15055 Genome accession   NZ_CP097858
Coordinates   3275099..3276139 (-) Length   346 a.a.
NCBI ID   WP_005436511.1    Uniprot ID   A0A0C1VE31
Organism   Vibrio owensii strain GL-605     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3270099..3281139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8C01_RS15025 endA 3270194..3270889 (+) 696 WP_264403813.1 deoxyribonuclease I -
  M8C01_RS15030 rsmE 3271036..3271767 (+) 732 WP_264403814.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M8C01_RS15035 gshB 3271782..3272732 (+) 951 WP_005425823.1 glutathione synthase -
  M8C01_RS15040 - 3272861..3273424 (+) 564 WP_005436504.1 YqgE/AlgH family protein -
  M8C01_RS15045 ruvX 3273475..3273900 (+) 426 WP_005436506.1 Holliday junction resolvase RuvX -
  M8C01_RS15050 pilU 3273961..3275067 (-) 1107 WP_005436509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M8C01_RS15055 pilT 3275099..3276139 (-) 1041 WP_005436511.1 type IV pilus twitching motility protein PilT Machinery gene
  M8C01_RS15060 - 3276168..3276878 (+) 711 WP_264403818.1 YggS family pyridoxal phosphate-dependent enzyme -
  M8C01_RS15065 proC 3277009..3277827 (+) 819 WP_038897097.1 pyrroline-5-carboxylate reductase -
  M8C01_RS15070 - 3277881..3278438 (+) 558 WP_005425806.1 YggT family protein -
  M8C01_RS15075 yggU 3278438..3278728 (+) 291 WP_264403819.1 DUF167 family protein YggU -
  M8C01_RS15080 - 3278788..3279219 (+) 432 WP_005425804.1 DUF4426 domain-containing protein -
  M8C01_RS15085 - 3279325..3279927 (+) 603 WP_005436525.1 XTP/dITP diphosphatase -
  M8C01_RS15090 hemW 3279946..3281118 (+) 1173 WP_264403820.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38661.14 Da        Isoelectric Point: 6.5043

>NTDB_id=693121 M8C01_RS15055 WP_005436511.1 3275099..3276139(-) (pilT) [Vibrio owensii strain GL-605]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=693121 M8C01_RS15055 WP_005436511.1 3275099..3276139(-) (pilT) [Vibrio owensii strain GL-605]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAGCTGGGCATTCCAGCGTTTACACACCAAGAAGTGCACCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCTGTGTTCCGTACCATCCCAAGCAGCATTCCAACACTGGA
AGAGCTAGAAGCTCCAGAGATCTTCAGAAAAATCGCTAACGCCGAGAAAGGTCTGGTACTGGTTACTGGTCCAACGGGTT
CTGGTAAATCGACGACCTTGGCAGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACATCCTAACAATTGAAGAT
CCAATTGAATTTGTTCACAACAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCGCTGCGTAGCGCATTGCGTGAAGACCCGGATGTGATTCTAGTCGGTGAGATGCGTGACAAAGAAACCATCAGTCTAG
CGCTGACAGCGGCTGAAACCGGTCACCTTGTTTTCGGTACACTACACACGAGTTCTGCGGCGAAAACCATCGACCGTATT
ATCGATGTGTTCCCGGGGAGCGATAAAGACATGGTGCGCTCGATGTTGTCGGAATCTTTGCGTTCGGTTATCGCGCAAAA
ACTTTTGAAACGTAACGGCGGCGGTCGTATTGCGTGTCATGAAATCATGATGGCAACACCGGCGATCCGAAACCTTATTC
GTGAAGATAAAGTCGCGCAGATGTACTCGATCATTCAGACCGGTGCGGCGCACGGCATGCAGACTATGGAACAAAATGCT
CGTCAATTGATGGCGCAAGGCATGGTATCGCGTGAAGAAGTGGATAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C1VE31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

72.424

95.376

0.691
  pilT Acinetobacter baumannii strain A118

72.424

95.376

0.691
  pilT Acinetobacter nosocomialis M2

72.121

95.376

0.688
  pilT Acinetobacter baylyi ADP1

71.818

95.376

0.685
  pilT Pseudomonas stutzeri DSM 10701

69.322

97.977

0.679
  pilT Pseudomonas aeruginosa PAK

69.118

98.266

0.679
  pilT Legionella pneumophila strain Lp02

67.976

95.665

0.65
  pilT Legionella pneumophila strain ERS1305867

67.976

95.665

0.65
  pilT Neisseria meningitidis 8013

64.412

98.266

0.633
  pilT Neisseria gonorrhoeae MS11

64.118

98.266

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.143

100

0.506
  pilU Vibrio cholerae strain A1552

41.916

96.532

0.405
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361