Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M8C01_RS15050 Genome accession   NZ_CP097858
Coordinates   3273961..3275067 (-) Length   368 a.a.
NCBI ID   WP_005436509.1    Uniprot ID   A0A812FD73
Organism   Vibrio owensii strain GL-605     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3268961..3280067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8C01_RS15020 - 3269546..3270043 (+) 498 WP_180824516.1 SprT family zinc-dependent metalloprotease -
  M8C01_RS15025 endA 3270194..3270889 (+) 696 WP_264403813.1 deoxyribonuclease I -
  M8C01_RS15030 rsmE 3271036..3271767 (+) 732 WP_264403814.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M8C01_RS15035 gshB 3271782..3272732 (+) 951 WP_005425823.1 glutathione synthase -
  M8C01_RS15040 - 3272861..3273424 (+) 564 WP_005436504.1 YqgE/AlgH family protein -
  M8C01_RS15045 ruvX 3273475..3273900 (+) 426 WP_005436506.1 Holliday junction resolvase RuvX -
  M8C01_RS15050 pilU 3273961..3275067 (-) 1107 WP_005436509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M8C01_RS15055 pilT 3275099..3276139 (-) 1041 WP_005436511.1 type IV pilus twitching motility protein PilT Machinery gene
  M8C01_RS15060 - 3276168..3276878 (+) 711 WP_264403818.1 YggS family pyridoxal phosphate-dependent enzyme -
  M8C01_RS15065 proC 3277009..3277827 (+) 819 WP_038897097.1 pyrroline-5-carboxylate reductase -
  M8C01_RS15070 - 3277881..3278438 (+) 558 WP_005425806.1 YggT family protein -
  M8C01_RS15075 yggU 3278438..3278728 (+) 291 WP_264403819.1 DUF167 family protein YggU -
  M8C01_RS15080 - 3278788..3279219 (+) 432 WP_005425804.1 DUF4426 domain-containing protein -
  M8C01_RS15085 - 3279325..3279927 (+) 603 WP_005436525.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41281.39 Da        Isoelectric Point: 6.5939

>NTDB_id=693120 M8C01_RS15050 WP_005436509.1 3273961..3275067(-) (pilU) [Vibrio owensii strain GL-605]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVAVLLDSAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEELKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKTTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEGFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=693120 M8C01_RS15050 WP_005436509.1 3273961..3275067(-) (pilU) [Vibrio owensii strain GL-605]
ATGGATCTGAATAAATTTCTCGAAGGCATGTTGGCGTTAAAAGCGTCGGATCTCTATATCACTGTTGGTGCGCCGATCTT
GTTTCGTGTCGATGGCGAACTGCGCCCGCAAGGCGATAAGTTGACTGAAAATGATGTGGCTGTGTTACTCGATAGTGCGA
TGGATCCGGAGCGACGTCAGGAGTTTCGTAAAAGCCGTGAGTCGAACTTTGCCATTGTCCGTGATTGCGGTCGTTTCCGT
GTCAGTGCCTTCTTCCAACGTGAATTGCCTGGCGCGGTGATTCGTCGTATCGAGACCAATATTCCGACTTTTGAAGAACT
TAAACTTCCTTTGGTTCTACAAGACCTTGCGATTGCAAAGCGTGGCTTGGTGCTGGTGGTTGGTGCAACAGGTTCTGGTA
AATCAACGACTATGGCCGCGATGACTGGCTACCGTAACAGCAATAAAACGGGGCATATTCTTACGGTAGAAGACCCGATT
GAATTTGTGCACGAGCATAAGCGTTGTATTGTGACTCAGCGTGAAGTGGGCTTGGATACCGACAGCTATGAAGTGGCATT
GAAGAACTCACTACGACAAGCGCCAGACATGATTTTGATTGGTGAGATTCGTAGCCGAGAAACGATGGAGTATGCAATGA
CCTTTGCTGAGACGGGCCACTTGTGTATGGCGACACTGCACGCCAACAATGCAAACCAAGCACTTGAGCGTATTCTGCAC
TTGGTGCCGAAAGACCAGAAAGATCAGTTCCTGTTTGATCTATCGATGAACTTAAAAGGCGTGGTAGGTCAGCAATTGAT
CCGTGATAAGAACGGTCAAGGTCGTCACGGTGTGTTTGAGATCTTATTAAACAGTCCACGCGTTTCGGATCTGATCCGCC
GTGGTGATCTGCATGAGTTGAAAACGACCATGGCACGCTCGAATGAATTTGGGATGCTGACCTTTGACCAGTCACTCTAC
AAGCTAGTGATGCAAGGTAAGATCAGTGAAGAGGACGCATTGCACAGTGCTGATTCCGCTAACGATCTGCGTTTGATGTT
GAAGACGCAACGCGGTGAAGGCTTCTCTACGGGCACGCTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A812FD73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.793

100

0.818
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.57

93.478

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.351

92.935

0.375
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37