Detailed information NTG0691821

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: M9O76_RS04645
• Genome accession: NZ_CP097711
• Coordinates: 942488..943468 (+)
• Length: 326 a.a.
• NCBI ID: WP_001467825.1
• Uniprot ID: -
• Organism: Escherichia coli strain MS1737
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 937488..948468

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M9O76_RS04615 (M9O76_04610)	yggM	938093..939100 (+)	1008	WP_000745232.1	DUF1202 family protein	-
M9O76_RS04620 (M9O76_04615)	hemW	939165..940301 (-)	1137	WP_000239951.1	radical SAM family heme chaperone HemW	-
M9O76_RS04625 (M9O76_04620)	rdgB	940294..940887 (-)	594	WP_001174738.1	XTP/dITP diphosphatase	-
M9O76_RS04630 (M9O76_04625)	yggU	940895..941185 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
M9O76_RS04635 (M9O76_04630)	yggT	941182..941748 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
M9O76_RS04640 (M9O76_04635)	yggS	941766..942470 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
M9O76_RS04645 (M9O76_04640)	pilT	942488..943468 (+)	981	WP_001467825.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M9O76_RS04650 (M9O76_04645)	ruvX	943642..944058 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
M9O76_RS04655 (M9O76_04650)	yqgE	944058..944621 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
M9O76_RS04660 (M9O76_04655)	gshB	944730..945680 (-)	951	WP_000593256.1	glutathione synthase	-
M9O76_RS04665 (M9O76_04660)	rsmE	945693..946424 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
M9O76_RS04670 (M9O76_04665)	endA	946504..947211 (-)	708	WP_001305312.1	deoxyribonuclease I	-
M9O76_RS04675 (M9O76_04670)	yggI	947306..947803 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 36073.30 Da
• Isoelectric Point: 5.9975

>NTDB_id=691821 M9O76_RS04645 WP_001467825.1 942488..943468(+) (pilT) [Escherichia coli strain MS1737]
MNMEEIVTLSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=691821 M9O76_RS04645 WP_001467825.1 942488..943468(+) (pilT) [Escherichia coli strain MS1737]
ATGAATATGGAAGAAATTGTGACCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	39.514	100	0.399
pilU	Pseudomonas stutzeri DSM 10701	37.576	100	0.38
pilU	Acinetobacter baylyi ADP1	36.957	98.773	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362