Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MW290_RS31760 Genome accession   NZ_CP097636
Coordinates   3750837..3751880 (+) Length   347 a.a.
NCBI ID   WP_250198319.1    Uniprot ID   -
Organism   Aquincola tertiaricarbonis strain RN12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3745837..3756880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW290_RS31740 (MW290_31735) - 3746944..3747831 (+) 888 WP_250198316.1 branched-chain amino acid ABC transporter permease -
  MW290_RS31745 (MW290_31740) - 3747848..3748822 (+) 975 WP_250200150.1 branched-chain amino acid ABC transporter permease -
  MW290_RS31750 (MW290_31745) - 3748819..3750060 (-) 1242 WP_250198317.1 DUF2254 domain-containing protein -
  MW290_RS31755 (MW290_31750) - 3750090..3750788 (-) 699 WP_250198318.1 YggS family pyridoxal phosphate-dependent enzyme -
  MW290_RS31760 (MW290_31755) pilT 3750837..3751880 (+) 1044 WP_250198319.1 type IV pilus twitching motility protein PilT Machinery gene
  MW290_RS31765 (MW290_31760) - 3751893..3752513 (+) 621 WP_250198320.1 cyclic nucleotide-binding domain-containing protein -
  MW290_RS31770 (MW290_31765) pilU 3752556..3753692 (+) 1137 WP_250198321.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MW290_RS31775 (MW290_31770) - 3753746..3754594 (+) 849 WP_250198322.1 hypothetical protein -
  MW290_RS31780 (MW290_31775) - 3754625..3755266 (-) 642 WP_250198323.1 BON domain-containing protein -
  MW290_RS31785 (MW290_31780) - 3755263..3755862 (-) 600 WP_250198324.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38101.70 Da        Isoelectric Point: 7.0666

>NTDB_id=691381 MW290_RS31760 WP_250198319.1 3750837..3751880(+) (pilT) [Aquincola tertiaricarbonis strain RN12]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEALDHKTVHDMVYDIMNDAQRKLYEDTLECDFSFEIQGLAR
FRVNAYNQNRGAGAVFRTIPSKILTLEQLQAPKVFADLALRPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHVLTVED
PIEFVHESKKCLINQREVGPHTLSFSNALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKDMVRAMLSESLVAVISQTLCKTRDGSGRVAAHEIMIANSAIKNLIRENKIAQMYSAIQTGQGLGMQTLDQN
LADLVRRNVITPAEARSKAKAPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=691381 MW290_RS31760 WP_250198319.1 3750837..3751880(+) (pilT) [Aquincola tertiaricarbonis strain RN12]
ATGGATATCACCCAGCTGCTGGCGTTTTCGGTCAAGAACAAGGCCTCCGACCTGCACTTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAACGTGGAGGCGCTGGACCACAAGACCGTGCACGACATGGTGTACGACA
TCATGAACGACGCCCAGCGCAAGCTCTACGAGGACACGCTGGAATGCGACTTCTCGTTCGAGATCCAGGGTCTGGCGCGT
TTCCGGGTCAACGCCTACAACCAGAACCGCGGTGCCGGCGCGGTGTTCCGCACCATTCCCAGCAAGATCCTGACGCTGGA
GCAGTTGCAGGCGCCCAAGGTGTTCGCCGACCTGGCGCTGCGCCCGCGCGGCCTGGTACTGGTCACCGGTCCCACCGGCT
CGGGCAAGAGCACCACGCTGGCGGCCATGGTCAACCACCTGAACGAAAACGAGTACGGCCACGTGCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAGAGCAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGCACACGCTGAGCTTCTCGAA
CGCGCTGCGCTCGGCCCTGCGTGAAGACCCGGACGCCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTCG
CGCTCACCGCAGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCAGCAGCGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGCGGCCGAGAAGGACATGGTGCGCGCGATGCTGTCCGAATCGCTGGTGGCGGTGATCTCGCAGAC
GCTGTGCAAGACCCGCGACGGCAGCGGCCGTGTGGCGGCGCACGAGATCATGATCGCCAACTCGGCCATCAAGAACCTGA
TCCGCGAGAACAAGATCGCCCAGATGTACTCCGCCATCCAGACCGGCCAGGGCCTGGGCATGCAGACGCTGGACCAGAAC
CTGGCCGACCTGGTGCGCCGCAACGTCATCACCCCGGCCGAAGCGCGCAGCAAGGCCAAGGCCCCCGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Legionella pneumophila strain Lp02

69.912

97.695

0.683
  pilT Legionella pneumophila strain ERS1305867

69.912

97.695

0.683
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.456

97.406

0.657
  pilT Vibrio cholerae strain A1552

67.456

97.406

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

40.58

99.424

0.403
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398