Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MW290_RS31770 Genome accession   NZ_CP097636
Coordinates   3752556..3753692 (+) Length   378 a.a.
NCBI ID   WP_250198321.1    Uniprot ID   -
Organism   Aquincola tertiaricarbonis strain RN12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3747556..3758692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW290_RS31745 (MW290_31740) - 3747848..3748822 (+) 975 WP_250200150.1 branched-chain amino acid ABC transporter permease -
  MW290_RS31750 (MW290_31745) - 3748819..3750060 (-) 1242 WP_250198317.1 DUF2254 domain-containing protein -
  MW290_RS31755 (MW290_31750) - 3750090..3750788 (-) 699 WP_250198318.1 YggS family pyridoxal phosphate-dependent enzyme -
  MW290_RS31760 (MW290_31755) pilT 3750837..3751880 (+) 1044 WP_250198319.1 type IV pilus twitching motility protein PilT Machinery gene
  MW290_RS31765 (MW290_31760) - 3751893..3752513 (+) 621 WP_250198320.1 cyclic nucleotide-binding domain-containing protein -
  MW290_RS31770 (MW290_31765) pilU 3752556..3753692 (+) 1137 WP_250198321.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MW290_RS31775 (MW290_31770) - 3753746..3754594 (+) 849 WP_250198322.1 hypothetical protein -
  MW290_RS31780 (MW290_31775) - 3754625..3755266 (-) 642 WP_250198323.1 BON domain-containing protein -
  MW290_RS31785 (MW290_31780) - 3755263..3755862 (-) 600 WP_250198324.1 SIS domain-containing protein -
  MW290_RS31790 (MW290_31785) - 3756020..3756970 (+) 951 WP_250198325.1 DUF5672 family protein -
  MW290_RS31795 (MW290_31790) - 3756868..3757287 (-) 420 WP_250198326.1 YraN family protein -
  MW290_RS31800 (MW290_31795) rsmI 3757286..3758194 (+) 909 WP_250198327.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41885.27 Da        Isoelectric Point: 6.8294

>NTDB_id=691382 MW290_RS31770 WP_250198321.1 3752556..3753692(+) (pilU) [Aquincola tertiaricarbonis strain RN12]
MERDQASKFVSDLLRLMIARGGSDLFLTAEFPPAIKVDGKITKVSPQPLTGQHTLALARALMNDKQAAEFERTKECNFAI
SPQGVGRFRCNAFVQQGQVGLVLRTIPATLPTIDGLNLPPVMKEVAMTKRGLVIFVGATGSGKSTSLAAMVDYRNENSHG
HIITIEDPVEFVHPHKNCIVTQREVGLDTDGWDIALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRVINFFPEERRQQLLMDLSLNLKALVSQRLLPRQEGKGRVAAVEILLNTPLVSDMIFKGEVGELKELMKRSRELG
MQTFDQALFDLFEAHAVTYEDALRNADSVNDLRLQIKLNSRRLRGADLASGTEHLTII

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=691382 MW290_RS31770 WP_250198321.1 3752556..3753692(+) (pilU) [Aquincola tertiaricarbonis strain RN12]
ATGGAAAGAGACCAGGCCTCCAAGTTCGTCAGCGATCTGCTGCGGTTGATGATCGCCCGCGGCGGCTCCGACCTCTTCCT
CACGGCCGAGTTTCCGCCCGCCATCAAGGTGGACGGCAAGATCACCAAGGTGTCGCCCCAGCCCCTGACCGGCCAGCACA
CGCTGGCGCTGGCGCGCGCGCTGATGAACGACAAGCAGGCCGCCGAGTTCGAGCGCACGAAGGAATGCAACTTCGCCATC
TCGCCGCAGGGCGTGGGGCGCTTCCGCTGCAATGCCTTCGTGCAGCAGGGCCAGGTGGGCCTGGTGCTGCGGACCATTCC
TGCCACGCTGCCCACCATCGACGGGCTCAACCTGCCGCCGGTGATGAAGGAAGTGGCGATGACCAAGCGCGGGCTGGTCA
TCTTCGTGGGCGCCACCGGCTCGGGCAAGAGCACCTCGCTGGCGGCGATGGTGGACTACCGCAACGAGAACTCGCACGGG
CACATCATCACCATCGAAGACCCGGTGGAGTTCGTGCACCCGCACAAGAACTGCATCGTCACCCAGCGCGAAGTGGGGCT
GGACACCGACGGATGGGACATCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATTCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAACATGCGGTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGCCACGCTGCATGCCAACAGCGCC
AACCAGGCGCTGGACCGGGTGATCAACTTCTTCCCGGAAGAGCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTCAACCT
CAAGGCGCTGGTCTCGCAACGGCTGCTGCCGCGCCAGGAGGGCAAGGGCCGTGTGGCCGCGGTGGAGATCCTGCTGAACA
CGCCGCTGGTGTCCGACATGATCTTCAAGGGCGAGGTGGGCGAGCTGAAGGAGCTGATGAAACGCAGCCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCCTTGTTCGACCTCTTCGAGGCCCACGCCGTGACCTACGAAGACGCGTTGCGCAACGCCGA
TTCGGTGAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCGCCGCCTGCGCGGCGCCGACCTGGCCTCGGGCACCGAGC
ACCTGACGATCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.836

96.561

0.616
  pilU Acinetobacter baylyi ADP1

59.429

92.593

0.55
  pilU Vibrio cholerae strain A1552

53.933

94.18

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.291

89.153

0.413
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter nosocomialis M2

42.342

88.095

0.373
  pilT Acinetobacter baumannii D1279779

43.574

84.392

0.368
  pilT Acinetobacter baumannii strain A118

43.574

84.392

0.368
  pilT Legionella pneumophila strain ERS1305867

41.441

88.095

0.365
  pilT Legionella pneumophila strain Lp02

41.441

88.095

0.365