Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5524_RS06265 Genome accession   NZ_CP097466
Coordinates   1458567..1459610 (-) Length   347 a.a.
NCBI ID   WP_373990588.1    Uniprot ID   -
Organism   Duganella sp. BuS-21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1453567..1464610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5524_RS06250 (M5524_06225) - 1454329..1456869 (-) 2541 WP_373990585.1 EAL domain-containing protein -
  M5524_RS06255 (M5524_06230) - 1456873..1457358 (-) 486 WP_373990586.1 glutathione peroxidase -
  M5524_RS06260 (M5524_06235) pilU 1457358..1458494 (-) 1137 WP_373990587.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5524_RS06265 (M5524_06240) pilT 1458567..1459610 (-) 1044 WP_373990588.1 type IV pilus twitching motility protein PilT Machinery gene
  M5524_RS06270 (M5524_06245) - 1459681..1460385 (+) 705 WP_373990589.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5524_RS06275 (M5524_06250) proC 1460389..1461210 (+) 822 WP_373990590.1 pyrroline-5-carboxylate reductase -
  M5524_RS06280 (M5524_06255) - 1461320..1462486 (-) 1167 WP_373990591.1 SO2930 family diheme c-type cytochrome -
  M5524_RS06285 (M5524_06260) - 1462483..1463745 (-) 1263 WP_373990592.1 parallel beta-helix domain-containing protein -
  M5524_RS06290 (M5524_06265) - 1463837..1464340 (-) 504 WP_373990593.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37965.61 Da        Isoelectric Point: 6.7265

>NTDB_id=689577 M5524_RS06265 WP_373990588.1 1458567..1459610(-) (pilT) [Duganella sp. BuS-21]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKAYEEMLEVDFSFAIPGLAR
FRVNAYNQERGASAVLRTIPSKILSLEDLNAPKIFADFALKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHILTIED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDCILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPADEKEMVRAMLSESLQAVISQTLLKTKDGTGRVAAHEIMVATPAIRNLIREAKIAQMYSAIQTGSGVGMQTLDQN
LTDLVRRNIISSAAARSAAKTPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=689577 M5524_RS06265 WP_373990588.1 1458567..1459610(-) (pilT) [Duganella sp. BuS-21]
ATGGACATCTCCGAACTACTCGCATTCTCTGTCAAGAACAAGGCCTCGGACCTGCACCTCTCGGCCGGCTTGCCGCCGAT
GATACGGGTACACGGCGACGTGCGGCGCATCAACCTGCCGCCGCTGGAGCACAAGGACGTCCACGGCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGGCCTACGAAGAGATGCTGGAGGTCGACTTCTCGTTTGCCATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCCTACAACCAGGAGCGCGGCGCCTCGGCGGTGCTGCGCACGATTCCGTCCAAGATCCTGTCGCTGGA
AGACCTCAACGCGCCCAAGATCTTCGCCGACTTCGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACCGGCCCGACCGGCT
CCGGCAAATCGACCACGCTGGCGGCCATGGTCAACCACCTCAACGAAAATGAATACGGCCACATCCTGACCATCGAAGAC
CCGATCGAATTCGTGCACGAATCCAAGAAATGCCTGATCAACCAGCGCGAAGTGGGACCGCACACGCTGTCGTTCAACAA
CGCGCTGCGCTCGGCGTTGCGTGAAGACCCGGACTGCATCCTGGTGGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGTCGGCGGCGGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGATGAAAAGGAAATGGTGCGGGCCATGCTGTCGGAATCGCTGCAGGCGGTGATCTCGCAGAC
GCTGCTGAAGACCAAGGACGGCACCGGCCGCGTGGCCGCGCATGAAATCATGGTCGCCACGCCGGCGATCCGCAACCTGA
TCCGCGAAGCCAAGATCGCCCAGATGTACTCGGCGATCCAGACCGGCAGCGGGGTCGGCATGCAAACGCTGGACCAGAAC
CTGACGGATCTGGTCCGCCGCAACATCATCAGCAGCGCCGCTGCCCGCAGCGCCGCCAAAACCCCCGAAAACTTTCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

75.221

97.695

0.735
  pilT Legionella pneumophila strain ERS1305867

75.221

97.695

0.735
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Neisseria meningitidis 8013

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.118

0.669
  pilT Vibrio cholerae strain A1552

68.843

97.118

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Pseudomonas stutzeri DSM 10701

42.284

93.372

0.395
  pilU Vibrio cholerae strain A1552

39.71

99.424

0.395