Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M5524_RS06260 Genome accession   NZ_CP097466
Coordinates   1457358..1458494 (-) Length   378 a.a.
NCBI ID   WP_373990587.1    Uniprot ID   -
Organism   Duganella sp. BuS-21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1452358..1463494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5524_RS06245 (M5524_06220) glcF 1453078..1454337 (+) 1260 WP_373990584.1 glycolate oxidase subunit GlcF -
  M5524_RS06250 (M5524_06225) - 1454329..1456869 (-) 2541 WP_373990585.1 EAL domain-containing protein -
  M5524_RS06255 (M5524_06230) - 1456873..1457358 (-) 486 WP_373990586.1 glutathione peroxidase -
  M5524_RS06260 (M5524_06235) pilU 1457358..1458494 (-) 1137 WP_373990587.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5524_RS06265 (M5524_06240) pilT 1458567..1459610 (-) 1044 WP_373990588.1 type IV pilus twitching motility protein PilT Machinery gene
  M5524_RS06270 (M5524_06245) - 1459681..1460385 (+) 705 WP_373990589.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5524_RS06275 (M5524_06250) proC 1460389..1461210 (+) 822 WP_373990590.1 pyrroline-5-carboxylate reductase -
  M5524_RS06280 (M5524_06255) - 1461320..1462486 (-) 1167 WP_373990591.1 SO2930 family diheme c-type cytochrome -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41821.39 Da        Isoelectric Point: 6.6667

>NTDB_id=689576 M5524_RS06260 WP_373990587.1 1457358..1458494(-) (pilU) [Duganella sp. BuS-21]
MERDQASKFMFDLLRLMLAKKGSDLFITAGFPPAVKIDGKMTPVSSQVLTAAHTLDLARSIMNDKQAANFELTKEANFAI
SPGDIGRFRVSAFVQMSSVGMVLRTITSEIPKLEDLGLPAVLKDVVMSKRGLVIMVGATGSGKSTTLAAMLGYRNENSYG
HIITIEDPVEYVHPHKNCIVTQREVGVDTDDWAVALKNTLRQAPDVIQIGEIRDRETMDHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPKKEAKGRAVAIEIMLNSPLISDLIFKGQVHEIKELMKKSRELG
MQTFDQSLFELHEADLITYEDALRNADSVNDLRLAIKLEGKAAKNRDLSAGTEHLGII

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=689576 M5524_RS06260 WP_373990587.1 1457358..1458494(-) (pilU) [Duganella sp. BuS-21]
ATGGAACGCGATCAGGCCTCTAAATTCATGTTCGACCTGCTGCGCCTGATGTTGGCCAAGAAGGGCTCGGACTTGTTCAT
CACCGCCGGCTTCCCGCCTGCCGTCAAGATCGACGGCAAGATGACGCCGGTGTCCTCGCAGGTGCTGACCGCCGCCCATA
CGCTCGACCTGGCCCGTTCCATCATGAACGACAAGCAGGCCGCCAACTTCGAGCTGACCAAGGAGGCCAACTTCGCCATC
AGCCCCGGCGATATCGGCCGCTTCCGCGTCTCGGCGTTTGTGCAGATGAGCTCGGTCGGCATGGTGCTGCGGACCATCAC
CTCCGAGATCCCCAAGCTGGAAGACCTCGGCCTGCCCGCAGTGCTCAAGGATGTGGTGATGTCCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACCGGTTCCGGCAAGTCGACCACCTTGGCGGCCATGCTCGGCTACCGCAACGAGAACAGCTACGGC
CATATCATCACCATCGAAGATCCGGTCGAATACGTCCACCCGCACAAGAACTGCATCGTCACCCAGCGCGAGGTCGGCGT
CGATACCGACGACTGGGCGGTGGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCGAAACCATGGACCACGCGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCGGAAGAGCGCCGCCAGCAACTGCTGATGGATTTGTCGCTCAACCT
GAAAGGCATGATTTCGCAGCGGCTGATTCCGAAGAAGGAAGCCAAGGGCCGCGCGGTGGCCATCGAGATCATGCTCAATT
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCCAGGTACACGAGATCAAGGAGCTGATGAAGAAATCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGTCACTGTTCGAACTGCATGAAGCTGACTTGATCACCTACGAAGACGCCCTGCGCAACGCCGA
CTCCGTCAACGACCTGCGCCTCGCCATCAAGCTGGAAGGCAAGGCCGCCAAGAACCGCGACCTGTCGGCCGGCACCGAAC
ATTTGGGAATCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(56-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.451

91.534

0.59
  pilU Acinetobacter baylyi ADP1

58.263

94.444

0.55
  pilU Vibrio cholerae strain A1552

57.185

90.212

0.516
  pilT Pseudomonas aeruginosa PAK

44.681

87.037

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.624

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.161

87.037

0.376
  pilT Acinetobacter nosocomialis M2

42.9

87.566

0.376
  pilT Acinetobacter baylyi ADP1

43.438

84.656

0.368
  pilT Acinetobacter baumannii D1279779

43.849

83.862

0.368
  pilT Acinetobacter baumannii strain A118

43.849

83.862

0.368
  pilT Legionella pneumophila strain ERS1305867

41.994

87.566

0.368
  pilT Legionella pneumophila strain Lp02

41.994

87.566

0.368